Review
Pharmacology & Pharmacy
Jian Lu, Jie Liu, Yuanqing Guo, Yuanjin Zhang, Yeye Xu, Xin Wang
Summary: The 2020 Nobel Prize in Chemistry was awarded to CRISPR-Cas9 for its super-selective and precise gene editing capabilities, which have great potential in disease treatment and animal model construction. CRISPR-Cas9 has been instrumental in establishing various rat models for drug metabolism and pharmacokinetics, leading to advancements in drug metabolism, chemical toxicity, and carcinogenicity studies. Challenges in this field have been identified, with proposed strategies to address them.
ACTA PHARMACEUTICA SINICA B
(2021)
Article
Multidisciplinary Sciences
Lin Ling, Alexander S. Raikhel
Summary: Female mosquitoes feed on carbohydrates and proteins during each gonadotrophic cycle to become reproductively competent, with metabolism synchronized to support this process. The hormones juvenile hormone and ecdysteroids play key roles in female mosquito reproduction, while insulin-like peptides are critical for controlling metabolism. The JH and 20E pathways regulate protein levels of ILPs, establishing a regulatory axis for metabolism during gonadotrophic cycles.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
News Item
Multidisciplinary Sciences
Max Kozlov
Summary: The study shows that the survival time for pig organ transplant is one of the longest, bringing pig organs closer to being used in human transplants.
Article
Medicine, Research & Experimental
Xi Chen, Zhiwei Zou, Qianli Wang, Wenwen Gao, Sisi Zeng, Shuangyan Ye, Pengfei Xu, Mengqiu Huang, Keyi Li, Jianping Chen, Zhuo Zhong, Qianbing Zhang, Bingtao Hao, Qiuzhen Liu
Summary: In this study, the CRISPR/Cas9 system was used to knock out the NOS1 gene and it was found that NOS1 knockout could inhibit the proliferation and tumor growth of melanoma cells. NOS1 knockout also increased the infiltration of immune cells in tumors and had effects on metabolic functions and immune response pathways.
JOURNAL OF TRANSLATIONAL MEDICINE
(2022)
Article
Plant Sciences
Jiarui Jiang, Haitao Huang, Qian Gao, Yong Li, Haiying Xiang, Wanli Zeng, Li Xu, Xin Liu, Jing Li, Qili Mi, Lele Deng, Wenwu Yang, Jianduo Zhang, Guangyu Yang, Xuemei Li
Summary: This study investigates the broad impact of DFR knockout on various developmental phases and organs of tobacco. DFR-KO decreases catabolism and photosynthetic light reactions in tobacco leaves during the flowering stage, while increasing carbon fixation and disease resistance pathways. However, the effects of DFR-KO on tobacco growth progressively decline with time. This research provides valuable insights and theoretical foundations for tobacco breeding and the development of gene-edited strains.
Review
Virology
Shuai Zhen, Hong Chen, Jiaojiao Lu, Xiling Yang, Xiaoqian Tuo, Shixue Chang, Yuhan Tian, Xu Li
Summary: The increasing incidence of sexually transmitted diseases in women has led to the need for user-friendly prevention methods. Vaginal delivery of CRISPR/Cas9 is a potential prevention method that involves silencing gene expression in a sequence-specific manner in the pathogenic agent. However, the vaginal mucosal barrier and physiological changes are obstacles to effective delivery and cell uptake of CRISPR/Cas9. Nano-carrier systems have been studied to enhance the vaginal delivery of CRISPR/Cas9. This paper describes various nano-carriers and their prospects in the preclinical stage, as well as the future significance of CRISPR/Cas9 vaginal delivery based on nano-carriers.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Multidisciplinary Sciences
Prasanta K. Dash, Chen Chen, Rafal Kaminski, Hang Su, Pietro Mancuso, Brady Sillman, Chen Zhang, Shuren Liao, Sruthi Sravanam, Hong Liu, Emiko Waight, Lili Guo, Saumi Mathews, Rahsan Sariyer, R. Lee Mosley, Larisa Y. Poluektova, Maurizio Caocci, Shohreh Amini, Santhi Gorantla, Tricia H. Burdo, Benson Edagwa, Howard E. Gendelman, Kamel Khalili
Summary: Treatment of HIV-1ADA-infected CD34+ NSG-humanized mice with long-acting ester prodrugs of cabotegravir, lamivudine, and abacavir in combination with native rilpivirine followed by dual CRISPR-Cas9 editing of CCR5 and HIV-1 proviral DNA led to elimination of replication-competent virus in 58% of infected mice and restoration of human CD4+ T cell numbers. The dual CRISPR therapy demonstrated statistically significant improvements in HIV-1 cure percentages compared to single treatments. Rating: 9/10
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Veterinary Sciences
Ping Jiang, Ambreen Iqbal, Mengyan Wang, Xiaohui Li, Xibi Fang, Haibin Yu, Zhihui Zhao
Summary: The study highlighted the critical regulatory role of ACADSB in lipid metabolism in bovine mammary epithelial cells, with its knockout resulting in reduced contents of CHOL, TGs, and FFAs, as well as altered expression of functional genes related to lipid metabolism. These findings underscore the importance of ACADSB in milk fat synthesis and provide valuable insights into gene regulatory roles and molecular mechanisms in this process.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Plant Sciences
Ya-Hui Wang, Yu-Qing Zhang, Rong-Rong Zhang, Fei-Yun Zhuang, Hui Liu, Zhi-Sheng Xu, Ai-Sheng Xiong
Summary: The DcLCYE gene is associated with carrot root color, with red carrots having lower expression levels compared to orange carrots. Red carrots accumulate more lycopene and less alpha-carotene.
Article
Pharmacology & Pharmacy
Xueyan Gou, Fenglin Ran, Jinru Yang, Yanrong Ma, Xin'an Wu
Summary: In this study, a Slc22a6/Slc22a8 double-knockout rat model was successfully generated using CRISPR/Cas9 technology. This model provides a useful tool for investigating the functions of OAT1/OAT3 in metabolic diseases, drug metabolism and pharmacokinetics, and OATs-mediated drug interactions.
Article
Medicine, Research & Experimental
Michael T. Meister, Marian J. A. Groot Koerkamp, Terezinha de Souza, Willemijn B. Breunis, Ewa Frazer-Mendelewska, Mariel Brok, Jeff DeMartino, Freek Manders, Camilla Calandrini, Hinri H. D. Kerstens, Alex Janse, M. Emmy M. Dolman, Selma Eising, Karin P. S. Langenberg, Marc van Tuil, Rutger R. G. Knops, Sheila Terwisscha van Scheltinga, Laura S. Hiemcke-Jiwa, Uta Flucke, Johannes H. M. Merks, Max M. van Noesel, Bastiaan B. J. Tops, Jayne Y. Hehir-Kwa, Patrick Kemmeren, Jan J. Molenaar, Marc van de Wetering, Ruben van Boxtel, Jarno Drost, Frank C. P. Holstege
Summary: This study successfully generated a collection of 19 pediatric RMS tumor organoid models, including all major subtypes. For aggressive tumors, the models can be established within a relatively short period of time and utilized for personalized drug screening. The models closely resemble the original tumors and maintain genetic stability over extended culture periods.
EMBO MOLECULAR MEDICINE
(2022)
Article
Cardiac & Cardiovascular Systems
Jaydev Dave, Nour Raad, Nishka Mittal, Lu Zhang, Anthony Fargnoli, Jae Gyun Oh, Maria Elisabetta Savoia, Jens Hansen, Marika Fava, Xiaoke Yin, Konstantinos Theofilatos, Delaine Ceholski, Erik Kohlbrenner, Dongtak Jeong, Lauren Wills, Mathieu Nonnenmacher, Kobra Haghighi, Kevin D. Costa, Irene C. Turnbull, Manuel Mayr, Chen-Leng Cai, Evangelia G. Kranias, Fadi G. Akar, Roger J. Hajjar, Francesca Stillitano
Summary: This study assessed the efficiency of in vivo genome editing using CRISPR/Cas9 and AAV9 in improving cardiac function in young adult mice with the human PLN-R14del mutation. The results showed that in vivo gene editing significantly reduced susceptibility to ventricular tachycardia and improved cardiac function in these mice, providing preclinical evidence for potential therapeutic interventions in patients with PLN-R14del mutation.
CARDIOVASCULAR RESEARCH
(2022)
Review
Chemistry, Multidisciplinary
Weiqi Cai, Tianli Luo, Lanqun Mao, Ming Wang
Summary: Recent innovations in genome editing have led to precise manipulation of genetic information in mammals using stimuli-responsive nanoparticles for the delivery and release of CRISPR/Cas9 genome editing machinery. These approaches can utilize biological signals or external signals to achieve improved genome editing outcomes.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Review
Chemistry, Physical
Zhaoyue Lv, Zhemian Li, Peiran Li, Chi Yao, Dayong Yang
Summary: The CRISPR/Cas system is an efficient gene editing tool, but the delivery of this system for therapeutics remains challenging. DNA nanomaterials, with their excellent molecular recognition capabilities, programmability, and biocompatibility, show promise as delivery carriers for gene agents. This review summarizes recent advances in DNA nanomaterials for CRISPR/Cas system delivery, categorizing them as either branched DNA-based nanostructures or rolling circle amplification (RCA)-based DNA nanostructures. Representative studies on the design of DNA nanomaterials as CRISPR/Cas system delivery carriers are highlighted, and the current challenges and opportunities for their development are discussed. It is anticipated that DNA nanomaterials will provide new possibilities for CRISPR/Cas-based therapeutics.
Article
Plant Sciences
Mengxi Bi, Zhijun Wang, Keyan Cheng, Yiqing Cui, Yi He, Jian Ma, Mingfang Qi
Summary: This study successfully applied the CRISPR/Cas9 large-scale screening strategy in tomato and constructed a CRISPR/Cas9 mutant library. The study found that homozygous and biallelic mutations occurred at higher frequencies in the mutant population. Additionally, a small-scale CRISPR/Cas9 library enhanced the efficacy of single sgRNA recognition in positive plants.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
