Article
Cell Biology
Anika Mijakovac, Azra Frkatovic, Maja Hanic, Jelena Ivok, Marina Martinic Kavur, Maja Pucic-Bakovic, Tim Spector, Vlatka Zoldos, Massimo Mangino, Gordan Lauc
Summary: Through genetic analysis of the TwinsUK female cohorts, we found that genetic factors have a moderate contribution to the variation of the glycan clock, while unique environmental factors have a minor role and shared environmental factors have a larger contribution. The estimates of genetic factors and unique environmental factors increased when age was included as a covariate.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Endocrinology & Metabolism
Valentina L. Greto, Ana Cvetko, Tamara Stambuk, Niall J. Dempster, Domagoj Kifer, Helena Deris, Ana Cindric, Frano Vuckovic, Mario Falchi, Richard S. Gillies, Jeremy W. Tomlinson, Olga Gornik, Bruno Sgromo, Tim D. Spector, Cristina Menni, Alessandra Geremia, Carolina V. Arancibia-Carcamo, Gordan Lauc
Summary: This study found that weight loss interventions can significantly affect IgG glycosylation, leading to reduced glycan levels and biological age.
INTERNATIONAL JOURNAL OF OBESITY
(2021)
Article
Medical Laboratory Technology
Wei Wang, Xuewen Xu, Chenjun Huang, Chunfang Gao
Summary: The study discovered distinct glycosylation patterns in serum and IgG of ITP patients compared to healthy controls, with IgG peak 7 showing potential as a biomarker for auxiliary diagnosis of ITP. Additionally, differences in serum fucosylation and IgG galactosylation were observed between ITP patients with severe thrombocytopenia and those with mild and moderate thrombocytopenia.
JOURNAL OF CLINICAL LABORATORY ANALYSIS
(2022)
Article
Chemistry, Analytical
Ahmed Ben Faleh, Stephan Warnke, Priyanka Bansal, Robert P. Pellegrinelli, Irina Dyukova, Thomas R. Rizzo
Summary: Glycan analysis has made significant progress in the past decade, with the development of high-resolution ion-mobility spectrometry allowing for the separation of structurally similar positional isomers. This study combines IMSn with cryogenic vibrational spectroscopy to identify the reducing-end anomers of N-glycans, and also establishes an infrared fingerprint database for unambiguous identification of these anomers from positional isomers in complex mixtures.
ANALYTICAL CHEMISTRY
(2022)
Article
Genetics & Heredity
Elena Bernabeu, Daniel L. McCartney, Danni A. Gadd, Robert F. Hillary, Ake T. T. Lu, Lee Murphy, Nicola Wrobel, Archie Campbell, Sarah E. Harris, David Liewald, Caroline Hayward, Cathie Sudlow, Simon R. Cox, Kathryn L. Evans, Steve Horvath, Andrew M. McIntosh, Matthew R. Robinson, Catalina A. Vallejos, Riccardo E. Marioni
Summary: This study developed accurate blood-based epigenetic predictors for both biological and chronological age through large-scale epigenome-wide association studies. The predictors were able to estimate an individual's age and their risk of mortality, providing important indicators of survival.
Article
Biochemical Research Methods
Barbara Radovani, Gordan Lauc, Ivan Gudelj
Summary: Immunoglobulin G (IgG) is an important antibody in the blood that plays a critical role in the immune defense against infectious agents. The glycosylation of IgG has been found to affect its functions and is associated with disease development and progression. Saliva is an easily collectible sample and could be a useful tool for studying the glycosylation of salivary IgG and its potential as a diagnostic biomarker. This study describes a method for analyzing the glycosylation of IgG from saliva samples using ultra-high-performance liquid chromatography, and compares it with the glycosylation profiles of IgG from plasma. The stability of salivary IgG glycosylation under different storage conditions and the effects of using a saliva preservation medium were also assessed.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Engineering, Multidisciplinary
Pengcheng Liu, Xiaobing Wang, Aishe Dun, Yutong Li, Houqiang Li, Lu Wang, Yichun Zhang, Cancan Li, Jinxia Zhang, Xiaoyu Zhang, Lixing Ma, Haifeng Hou
Summary: This study evaluated the diagnostic performance of IgG N-glycosylation in gastrointestinal cancer subtypes and found that it plays an important role in the pathogenesis of GI cancers. By analyzing the composition of plasma IgG N-glycans, the levels of inflammatory cytokines, and their correlation, a glycan-based model was developed for diagnosing GI cancers.
Article
Oncology
Sen Yang, Ming Cui, Qiaofei Liu, Quan Liao
Summary: Glycosylated IgG is associated with tumor progression and has potential applications in tumor diagnosis and treatment.
Article
Immunology
Di Liu, Jing Dong, Jie Zhang, Xizhu Xu, Qiuyue Tian, Xiaoni Meng, Lijuan Wu, Deqiang Zheng, Xi Chu, Wei Wang, Qun Meng, Youxin Wang
Summary: Observational studies have found a potential causal association between IgG N-glycosylation and rheumatoid arthritis (RA). Further research is needed to confirm this finding and explore the underlying physiological mechanisms.
JOURNAL OF IMMUNOLOGY
(2022)
Article
Medical Laboratory Technology
Jasminka Kristic, Gordan Lauc, Marijia Pezer
Summary: This review focuses on the changes in IgG glycosylation during aging in humans and discusses the potential mechanisms and functional roles of these changes.
CLINICA CHIMICA ACTA
(2022)
Article
Geriatrics & Gerontology
C. Elizabeth Shaaban, Caterina Rosano, Xiaonan Zhu, Bret R. Rutherford, Kailyn R. Witonsky, Andrea L. Rosso, Kristine Yaffe, Patrick J. Brown
Summary: Older adults with discordant biological and chronological ages may differ in cognitive and physical function from those with concordant ages. By creating participant groups based on medians of chronological age and a previously validated biological age index, we can assess these differences in a clinically accessible manner. The results show that older adults with larger age discordance perform worse in cognition and frailty, while those with smaller age discordance perform better.
JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES
(2023)
Article
Chemistry, Analytical
Anne-Lise Marie, Somak Ray, Shulin Lu, Jennifer Jones, Ionita Ghiran, Alexander R. Ivanov
Summary: A highly sensitive method for profiling N-glycans released from proteins using CZE-ESI-MS was developed and applied to glycan analysis of plasma and blood-derived isolates. The technique significantly improved detection sensitivity and resolution, allowing for qualitative and quantitative analysis of N-glycans in blood samples with unmatched depth compared to previous methods.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
P. Jinesh, P. Lijina, B. S. Gnanesh Kumar
Summary: In this study, buffalo milk IgG was characterized for its amino acid sequence and glycan diversity using reverse phase liquid chromatography coupled to ESI-Q-TOF MS in tandem mode. Two IgG subtypes, IgG1 and IgG3, were identified, with IgG1 being the predominant subtype. The observed glycan diversity in buffalo milk IgG is partly comparable with that of bovine colostrum as well as human, bovine, and goat serum counterparts.
Article
Biochemistry & Molecular Biology
Cristian D. Gutierrez Reyes, Md. Abdul Hakim, Mojgan Atashi, Mona Goli, Sakshi Gautam, Junyao Wang, Andrew I. Bennett, Jianhui Zhu, David M. Lubman, Yehia Mechref
Summary: This study identifies potential N-glycan markers for mild cognitive impairment (MCI) using LC-MS/MS glycomics approach. The identified N-glycans may play an important role in neuroinflammation.
Review
Medicine, General & Internal
Benjamin S. Haslund-Gourley, Brian Wigdahl, Mary Ann Comunale
Summary: IgG N-glycans have emerged as a source of disease-specific biomarkers. They can effectively discriminate between healthy individuals, autoimmune disease, infectious disease, and cancer with high sensitivity. Furthermore, they can be longitudinally collected to track treatment responses and predict disease reoccurrence.
Article
Respiratory System
Anna Guyatt, Catherine John, Alexander T. Williams, Nick Shrine, Nicola F. Reeve, Ian Sayers, Ian Hall, Louise Wain, Nuala Sheehan, Frank Dudbridge, Martin D. Tobin
Summary: This study investigated the causal relationship between eosinophils and various respiratory phenotypes. The findings suggest a potential causal link between elevated eosinophils and ACO, moderate-to-severe asthma, and reduced lung function. However, the heterogeneity of the results calls for caution, as other mechanisms may also contribute to respiratory health impairment.
Review
Surgery
Peige Song, Yazhou He, Davies Adeloye, Yuefeng Zhu, Xinxin Ye, Qian Yi, Kazem Rahimi, Igor Rudan, Global Hlth Epidemiol Res Grp GHERG
Summary: The study aimed to estimate the global and regional prevalence of abdominal aortic aneurysms (AAAs) in 2019 and evaluate associated factors. The global prevalence of AAA among people aged 30 to 79 years was 0.92%, with a total of 35.12 million AAA cases in 2019. Smoking, male sex, family history of AAA, advanced age, hypertension, hypercholesterolemia, obesity, cardiovascular disease, cerebrovascular disease, claudication, peripheral artery disease, pulmonary disease, and renal disease were associated with AAA. The Western Pacific region had the highest AAA prevalence, while the African region had the lowest prevalence.
Article
Biotechnology & Applied Microbiology
Jelena Simunovic, Jernej Gaspersic, Urh Cernigoj, Jana Vidic, Ales Strancar, Mislav Novokmet, Genadij Razdorov, Marija Pezer, Gordan Lauc, Irena Trbojevic-Akmacic
Summary: This study developed a high-throughput method for the enrichment of haptoglobin from human plasma, using monolithic chromatographic support and fluorescent detection technology. The method allows for fast, efficient, and robust analysis of haptoglobin glycosylation.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Article
Medicine, General & Internal
Yulong Lan, Guanzhi Chen, Dan Wu, Xiong Ding, Zegui Huang, Xianxuan Wang, Lois Balmer, Xingang Li, Manshu Song, Wei Wang, Shouling Wu, Youren Chen
Summary: This study analyzed the relationship between atherogenic dyslipidemia, inflammation, and the future development of type 2 diabetes in individuals with overt hyperglycemia. The study found that there is a temporal correlation between high-sensitivity C-reactive protein (hsCRP) and the atherogenic index of plasma (AIP), and cumulative exposure to these factors increases the risk of developing diabetes. Therefore, there is a need for comprehensive assessment and management of chronic inflammation and atherogenic dyslipidemia in individuals with subclinical risks of type 2 diabetes.
Article
Engineering, Multidisciplinary
Xiaoni Meng, Weijie Cao, Di Liu, Isinta Maranga Elijah, Weijia Xing, Haifeng Hou, Xizhu Xu, Manshu Song, Youxin Wang
Summary: Mendelian randomization analysis revealed a bidirectional causal association between IgG N-glycosylation and metabolic traits.
Article
Biochemistry & Molecular Biology
Lucija Tudor, Gordana Nedic Erjavec, Matea Nikolac Perkovic, Marcela Konjevod, Suzana Uzun, Oliver Kozumplik, Ninoslav Mimica, Gordan Lauc, Dubravka Svob Strac, Nela Pivac
Summary: The molecular mechanisms behind PTSD, including the role of glycosylation and the FUT8 gene, were investigated in this study. Associations between plasma N-glycan levels and FUT8-related polymorphisms were observed in both PTSD patients and control participants. The findings suggest that FUT8-related polymorphisms play a regulatory role in glycosylation, which may contribute to the development and clinical manifestation of PTSD.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Virology
Xiaoqian Xu, Hao Wang, Shan Shan, Yameng Sun, Xiaoyuan Xu, Hong You, Jidong Jia, Hui Zhuang, Yuanyuan Kong
Summary: The study aims to explore the impact of different definitions of clinical phases of chronic hepatitis B (CHB) infection on grey zone patients, based on HBV guidelines by the CSH/CSID and AASLD. The retrospective examination included untreated CHB patients from the China Registry of Hepatitis B database, with clinical phases determined by the CSH/CSID and AASLD. The results showed that the CSH/CSID guidelines leave fewer and less severe patients in the grey zone compared to the AASLD guidelines.
Article
Endocrinology & Metabolism
Dinko Soic, Jerko Stambuk, Marko Tijardovic, Toma Keser, Gordan Lauc, Tomislav Bulum, Marijana Vucic Lovrencic, Sandra Vuckovic Rebrina, Martina Tomic, Mislav Novokmet, Lea Smircic-Duvnjak, Olga Gornik
Summary: This study investigated the association between C3 N-glycosylation and complications of type 1 diabetes, and found significant changes in C3 N-glycome in severe albuminuria and hypertension accompanying the disease. All C3 glycopeptides except one were associated with HbA1c levels, and one glycoform was associated with non-proliferative diabetic retinopathy. The results suggest that C3 N-glycosylation is a potential novel marker for the progression and severity of type 1 diabetes.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Barbara Radovani, Frano Vuckovic, Aldo P. Maggioni, Ele Ferrannini, Gordan Lauc, Ivan Gudelj
Summary: This study investigated the association between N-glycosylation of immunoglobulin G (IgG) and coronary artery disease (CAD). The analysis revealed differences in IgG N-glycome composition between CAD+ and CAD- cases, particularly in women, where sialylated N-glycan structures were negatively associated with CAD.
Article
Biochemistry & Molecular Biology
Branimir Plavsa, Janko Szavits-Nossan, Aleksandar Blivajs, Borna Rapcan, Barbara Radovani, Igor Sesto, Kresimir Stambuk, Vito Mustapic, Lovorka Derek, Diana Rudan, Gordan Lauc, Ivan Gudelj
Summary: Atrial fibrillation is a complex disease with aberrant electrical signaling and the development of a susceptible heart substrate. The presence of inflammation, characterized by changes like adipose tissue accumulation and interstitial fibrosis, plays a crucial role. This study investigated the N-glycosylation patterns in atrial fibrillation patients and found significant differences in plasma N-glycome and IgG N-glycans between the case and control groups. Additionally, specific N-glycans were associated with atrial fibrillation recurrence and correlated with the CHA(2)DS(2)-VASc score.
Article
Biochemistry & Molecular Biology
Doris Plecas, Nikol Mraz, Anne Marie Patanaude, Tea Pribic, Ivana Pavlinac Dodig, Renata Pecotic, Gordan Lauc, Ozren Polasek, Zoran Dogas
Summary: This study aimed to investigate the potential use of N-glycome as a biomarker for severe obstructive sleep apnea (OSA). By comparing 70 subjects with AHI over 30 to 23 controls with AHI under 5, significant associations were found between three plasma glycan peaks, one IgG glycan peak, and severe OSA. Additionally, patients with severe OSA showed accelerated biological aging. In conclusion, plasma N-glycome can serve as a biomarker for severe OSA.
Article
Gastroenterology & Hepatology
Nicholas T. Ventham, Nicholas A. Kennedy, Rahul Kalla, Alex T. Adams, Alexandra Noble, Holly Ennis, T. O. P. P. I. C. Study Group, Ibd-biom Consortium, Craig Mowat, Malcolm G. Dunlop, Jack Satsangi
Summary: This study aimed to investigate the DNA methylation alterations associated with Crohn's disease (CD) recurrence following surgery. The results showed differentially methylated positions and differentially variable positions in patients with CD recurrence. The study also validated the previously reported CD-associated methylome.
CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY
(2023)
Review
Biotechnology & Applied Microbiology
Sofia Shkunnikova, Anika Mijakovac, Lucija Sironic, Maja Hanic, Gordan Lauc, Marina Martinic Kavur
Summary: Immunoglobulin (IgG) glycosylation is a crucial process for the structure and function of IgG. Changes in IgG glycome have been associated with aging, pollution, diseases, and inflammation. IgG N-glycosylation fine-tunes the immune response and can be used as a biomarker and evaluation tool in various health interventions.
BIOTECHNOLOGY ADVANCES
(2023)
Article
Cell Biology
Marko Marjanovic, Ana-Matea Mikecin Drazic, Marija Mioc, Mladen Paradzik, Filip Klicek, Mislav Novokmet, Gordan Lauc, Marijeta Kralj
Summary: Targeting the Golgi apparatus could be a novel therapeutic approach against CSCs, as it selectively eliminates EMT cells.
JOURNAL OF CELL SCIENCE
(2023)
Meeting Abstract
Cardiac & Cardiovascular Systems
Janko Szavits Nossan, Barbara Radovani, Gordan Lauc, Ivan Gudelj
JOURNAL OF CARDIOVASCULAR ELECTROPHYSIOLOGY
(2023)