Article
Biotechnology & Applied Microbiology
Jiasheng Huang, E. Zixuan, Wenjie Pan, Zhi Li, Tiehao Lin, Chunhua Ren, Peng Luo, Bo Ma, Yang Liu, Xugan Wu, Chaoqun Hu, Xiao Jiang, Ting Chen
Summary: In this study, liquid chromatography-tandem mass spectrometry (LC-MS/MS) and Next-Generation sequencing (NGS) were used to investigate the changes in metabolomic and transcriptomic profiles during pigmentation in the sea cucumber Holothuria leucospilota. The analysis identified differentially regulated metabolites and expressed genes, suggesting notable alterations in nutritional status rather than the classical melanin pathway during the pigmentation process.
MARINE BIOTECHNOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Regina Zhi Ling Leong, Lai Huat Lim, Yik Ling Chew, Swee Sen Teo
Summary: Sea cucumber, as a bioremediator, synthesizes organic matter and excretes inorganic matter. It has the potential to serve as a bioindicator in marine habitats. The study provides transcriptome data for sea cucumber, which is crucial in identifying genes related to the bioindication of sea cucumber in a high copper environment.
ANIMAL BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ziqi Wang, Yali Shi, Shiyu Zeng, Yuanping Zheng, Huaijie Wang, Haihui Liao, Jie Song, Xinyue Zhang, Jun Cao, Chuan Li
Summary: This study investigated the regulatory effects of Holothuria leucospilota polysaccharides (HLP), a bioactive component of marine resources, on loperamide-induced constipation. HLP treatment improved constipation symptoms, enhanced water-electrolyte metabolism, and increased gastrointestinal motility in constipated mice. The treatment also reduced intestinal barrier damage, inhibited inflammatory response, and improved the composition of intestinal microbiota, leading to increased production of short-chain fatty acids (SCFAs) in the intestine.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Food Science & Technology
Wanting Wang, Yiqiong Yuan, Jun Cao, Xuanri Shen, Chuan Li
Summary: The study found that HLP exhibited good fermentability both in vitro and in vivo, and significantly enhanced liver antioxidant activity in mice.
Article
Multidisciplinary Sciences
Ting Chen, Chunhua Ren, Nai-Kei Wong, Aifen Yan, Caiyun Sun, Dingding Fan, Peng Luo, Xiao Jiang, Lvping Zhang, Yao Ruan, Jiaxi Li, Xiaofen Wu, Da Huo, Jiasheng Huang, Xiaomin Li, Feifei Wu, E. Zixuan, Chuhang Cheng, Xin Zhang, Yanhong Wang, Chaoqun Hu
Summary: Some tropical sea cucumbers have the ability to repel or ensnare predators by ejecting a bioadhesive matrix called the Cuvierian organ (CO). The mechanisms for adhesiveness in CO and how sea cucumbers perceive and respond to danger signals for CO expulsion have been unclear. This study reports the genome assembly of Holothuria leucospilota, a sea cucumber with the prototypical CO. The genome reveals characteristic long-repeat signatures in CO-specific proteins and hints at the transduction of mechanical pressure onto the CO surface for expulsion.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Nutrition & Dietetics
Ruizhen Ru, Yanzheng Guo, Juanxuan Mao, Zonghe Yu, Wen Huang, Xudong Cao, Huijian Hu, Minjie Meng, Lihong Yuan
Summary: Marine organisms, including sea cucumbers, have shown promising anti-cancer effects. In this study, the anti-cancer effect of a protein extracted from the edible sea cucumber species Holothuria leucospilota (H. leucospilota) was investigated. The protein demonstrated concentration-dependent inhibition on the viability of various cancer cell lines, while showing no effects on normal cell lines. It also affected the cell cycle and induced apoptosis in cancer cells, and exhibited excellent performance in inhibiting cell migration. This protein shows potential as a promising anti-cancer drug.
Article
Food Science & Technology
Xin Zhang, Kexue Zhu, Shunjiang Zeng, Yuanping Zheng, Jun Cao, Chuan Li
Summary: This study aims to investigate the impact of Holothuria leucospilota polysaccharides (HLP) on DSS-induced colitis in rats and further investigate the complex interactions between changes in intestinal microbiota, cometabolites, and intestinal inflammation under HLP intervention. The results show that HLP can alleviate inflammation and reduce histological damage and secretion of pro-inflammatory cytokines. HLP also regulates the intestinal flora and restores flora diversity. Fecal metabolomics analysis reveals the modulatory effects of HLP on amino acid metabolism, antimicrobial peptide anabolism, and energy metabolism.
MOLECULAR NUTRITION & FOOD RESEARCH
(2023)
Article
Food Science & Technology
Fuqiang Zhao, Tingting Ma, Xin Zhang, Qiancheng Zhao, Kexue Zhu, Jun Cao, Zhongyuan Liu, Xuanri Shen, Chuan Li
Summary: This study investigates the regulatory effect of Holothuria leucospilota polysaccharides (HLP) on immunosuppressed mice. HLP treatment significantly increases immune organ indexes, serum cytokines, and immunoglobulin levels in immunosuppressed mice. It also regulates the gut microbiota composition and improves immune function through the activation of the TLR pathway. The findings suggest the potential of HLP as prebiotics in the development of novel immunopotentiators.
MOLECULAR NUTRITION & FOOD RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Changlin Li, Wang Zhao, Chuanxin Qin, Gang Yu, Zhenhua Ma, Yu Guo, Wanni Pan, Zhengyi Fu, Xingmei Huang, Jisheng Chen
Summary: The study revealed that protein synthesis and processing pathways are affected by heat stress, while lipid metabolism pathways are affected by cold stress. Heat shock proteins play significant roles in protecting sea cucumbers from high temperature stress.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
(2021)
Article
Fisheries
Jia-Yang He, Pin-Hong Li, Xi Huang, Yue-Hong Sun, Xiao-Peng He, Wei Huang, Zong-He Yu, Hong-Yan Sun
Summary: NF-kappa B1 p105 is a key protein in sea cucumber involved in regulating immune responses and inhibiting apoptosis, with important biological functions.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2021)
Article
Chemistry, Medicinal
Tanatcha Sanguanphun, Sukrit Promtang, Nilubon Sornkaew, Nakorn Niamnont, Prasert Sobhon, Krai Meemon
Summary: This study evaluated the beneficial effects of a compound derived from Holothuria leucospilota (H. leucospilota) on Parkinson's disease (PD) models. The compound, HLEA-P3, restored dopaminergic neuron viability, improved dopamine-dependent behaviors, reduced oxidative stress, and prolonged lifespan in PD worms induced by 6-hydroxydopamine (6-OHDA). HLEA-P3 also decreased α-synuclein aggregation and regulated genes involved in antioxidant, autophagy, and fatty acid metabolism pathways. Overall, these findings suggest the potential of HLEA-P3 as a nutritional therapy for treating PD.
Article
Fisheries
Lian Liu, Xiaomin Li, Xiaofen Wu, Juan Feng, Xiangxing Zhu, Dongsheng Tang, Xiao Jiang, Wenjie Pan, Jiasheng Huang, Ting Chen, Chunhua Ren, Aifen Yan
Summary: This study identifies 55 genes belonging to 7 families of the intrinsic apoptotic pathway in the tropical sea cucumber Holothuria leucospilota, confirming the conservation of the intrinsic apoptotic pathway between vertebrates and echinoderms. The Bcl-2 family shows significant gene expansion in H. leucospilota, while the pro-apoptotic factor Endo G is generally lost in echinoderms. Transcriptional data reveals the widespread expression of intrinsic apoptotic pathway genes in all tissues and developmental stages of H. leucospilota. The study also highlights the different responses of caspase genes in primary coelomocytes to pathogenic and environmental challenges.
AQUACULTURE REPORTS
(2023)
Article
Fisheries
Xiaoying Chen, Minwei Huang, Kai Peng, Yuping Sun, Xiaopeng Wu, Huijie Lu, Yehui Zhang, Huo Li, Guoxia Wang, Wen Huang
Summary: This study establishes a simple and successful technology for large-scale artificial breeding of Holothuria leucospilota, a tropical sea cucumber, through constant stimulation and dry shock. The best diet scheme for their development is a mixture of Chlorella pyrenoidosa powder, Saccharomyces cerevisiae yeast powder, and sea mud, with a feeding mode of 2:1:2 ratio.
Article
Food Science & Technology
Fuqiang Zhao, Kexue Zhu, Qiancheng Zhao, Qibing Liu, Jun Cao, Guanghua Xia, Zhongyuan Liu, Chuan Li
Summary: The study evaluated the beneficial effects of orally administered Holothuria leucospilota polysaccharides (HLP) on liver injury in diabetic rats. The results showed that HLP regulated liver function and reduced lipid accumulation in livers of diabetic rats. Additionally, HLP exhibited antioxidant and anti-inflammatory activities, repairing tissue damage in diabetic livers.
JOURNAL OF FUNCTIONAL FOODS
(2021)
Article
Fisheries
Zonghe Yu, Hong Wu, Youkai Tu, Zesen Hong, Jiewen Luo
Summary: The sea cucumber Holothuria leucospilota has become commercially important due to the overexploitation of other tropical sea cucumbers. This study investigated different ratios of microalgae and yeast as diets for H. leucospilota larvae and found that a combination of C. muelleri and S. cerevisiae at a 3:1 ratio resulted in optimal growth and development.
AQUACULTURE NUTRITION
(2022)
Article
Biochemistry & Molecular Biology
Diana Medina-Felix, Francisco Vargas-Albores, Estefania Garibay-Valdez, Luis Rafael Martinez-Cordova, Marcel Martinez-Porchas
Summary: In this research, the effects of Nocardia infection on fish gastrointestinal microbiota were analyzed. It was found that the infection led to decreased survival rate, severe damage to the stomach microbiota, and a significant increase in Proteobacteria. A negative correlation network between Proteobacteria and other important phyla was observed. Therefore, Nocardia sp. is an emerging pathogen capable of inducing dysbiosis and causing significant mortalities.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
(2024)
Article
Biochemistry & Molecular Biology
Lele Wu, Wen Sun, Jiale Zhou, Yaolin Li, Jun Li, Zongcheng Song, Changbin Song, Shihong Xu, Xinlu Yue, Xian Li
Summary: The study finds that red light induces dichromatic skin pigmentation in turbot juveniles, with some individuals displaying black coloration and others displaying lighter skin. The upregulated gene expressions related to melanin synthesis and the involvement of the nervous system in spectral environment-driven color regulation are both crucial factors.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
(2024)