Fluorine-modified bisbenzimide derivative as a molecular probe for bimodal and simultaneous detection of DNAs by F-19 NMR and fluorescence

3,5-Bis(trifluoromethyl) benzene modified bisbenzimide H 33258 was synthesized as a F-19 magnetic resonance-based DNA detection probe. The chemical shift and fluorescence of the probe were significantly changed by the addition of hairpin DNAs having an AATT sequence. The probe enables F-19 NMR/fluorescence bimodal detection of the model DNA double strands simultaneously.