Article
Biochemistry & Molecular Biology
Natalia L. Starodubtseva, Alisa O. Tokareva, Valeriy V. Rodionov, Alexander G. Brzhozovskiy, Anna E. Bugrova, Vitaliy V. Chagovets, Vlada V. Kometova, Evgenii N. Kukaev, Nelson C. Soares, Grigoriy I. Kovalev, Alexey S. Kononikhin, Vladimir E. Frankevich, Evgeny N. Nikolaev, Gennady T. Sukhikh
Summary: Metastasis is a serious and life-threatening condition in breast cancer patients. In this study, the serum proteome and lipidome profiles of BC patients were analyzed. The levels of certain proteins and lipids were found to be correlated with tumor grade, subtypes, and lymph node metastasis. Two accurate classifiers were developed to distinguish between metastatic and non-metastatic BC, achieving 90% accuracy for proteomic features and 80% accuracy for lipidomic features. The best classifier, based on logistic regression and the levels of 11 proteins, showed high sensitivity, specificity, and diagnostic performance.
Article
Cell Biology
Guillermo Solis-Fernandez, Ana Montero-Calle, Javier Martinez-Useros, Alvaro Lopez-Janeiro, Vivian de los Rios, Rodrigo Sanz, Jana Dziakova, Elena Milagrosa, Maria Jesus Fernandez-Acenero, Alberto Pelaez-Garcia, Jose Ignacio Casal, Johan Hofkens, Susana Rocha, Rodrigo Barderas
Summary: In this study, quantitative spatial proteomics analysis was used to investigate lymph nodes, liver, and lung metastasis in colorectal cancer (CRC). The results revealed dysregulation of protein abundance and localization in these metastatic sites, with proteins such as GLG1 and MUC5AC being associated with poor prognosis and diagnosis of CRC.
Article
Biochemistry & Molecular Biology
Kassondra Balestrieri, Kimberly Kew, Moses McDaniel, Mohamed Ramez, H. Keith Pittman, Gina Murray, Nasreen A. Vohra, Kathryn M. Verbanac
Summary: This study used proteomics to reveal high expression of Galectin-1 protein and RNA in primary and metastatic claudin-low breast cancer, providing insight into the mechanisms of tumor progression.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2021)
Article
Biochemistry & Molecular Biology
Juliana Pereira Lopes Goncalves, Christine Bollwein, Aurelia Noske, Anne Jacob, Paul Jank, Sibylle Loibl, Valentina Nekljudova, Peter A. A. Fasching, Thomas Karn, Frederik Marme, Volkmar Mueller, Christian Schem, Bruno Valentin Sinn, Elmar Stickeler, Marion van Mackelenbergh, Wolfgang D. D. Schmitt, Carsten Denkert, Wilko Weichert, Kristina Schwamborn
Summary: Immunohistochemical evaluation of breast cancer subtypes using estrogen receptor, progesterone receptor, and HER2 status is important for treatment determination. This study utilized mass spectrometry imaging to identify molecular features and accurately classify triple-negative breast cancer (TNBC). The models developed demonstrated high accuracy for hormone receptors and TNBC classification, and vimentin overexpression was found to be a potential target for diagnosis and treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Anna Daily, Prashanth Ravishankar, Steve Harms, V. Suzanne Klimberg
Summary: This study identified protein biomarkers with altered expression levels in tear samples from women diagnosed with breast cancer. Through LC-MS/MS analysis, 14 potential biomarkers were identified and validated by ELISA. The results showed significantly higher expression of S100A8 and S100A9 in breast cancer patients, while Galectin-3 binding protein had increased expression in the control group. These findings support the use of tear proteins in detecting systemic diseases such as breast cancer.
Article
Multidisciplinary Sciences
John P. McGee, Pei Su, Kenneth R. Durbin, Michael A. R. Hollas, Nicholas W. Bateman, G. Larry Maxwell, Thomas P. Conrads, Ryan T. Fellers, Rafael D. Melani, Jeannie M. Camarillo, Jared O. Kafader, Neil L. Kelleher
Summary: This study introduces a semi-automated single-ion mass spectrometry workflow for top-down tandem mass spectrometry analysis directly from tissue microenvironments. The results demonstrate that this method can identify multiple proteoforms in tumor samples within a short period of time, and effectively identify proteoform signatures in tumor and stromal regions.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Imrich Geci, Peter Bober, Eva Filova, Evzen Amler, Jan Sabo
Summary: Breast cancer is the most common cancer in women worldwide and its aggressive nature is attributed to weakened intercellular adhesion regulated by GTPases. In this study, we investigated the role of ARHGAP1 in the inactivation of Rho GTPases after doxorubicin treatment. Proteomic analysis revealed that ARHGAP1 inactivated RAC1, CDC42, and RHOA GTPases, leading to epithelial-mesenchymal transitions through the TGF-beta signaling pathway. These findings shed light on the molecular mechanism behind reduced cell adhesion and increased metastasis risk in drug-resistant breast cancer cells treated with doxorubicin.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Editorial Material
Engineering, Biomedical
Cheyenne Ernst, Hai Wang
Summary: In breast cancer metastasized to bone, the mineralization of collagen regulates integrin-mediated mechanosignalling, leading to a less proliferative phenotype in breast cancer cells.
NATURE BIOMEDICAL ENGINEERING
(2023)
Article
Biochemistry & Molecular Biology
Sini Li, Yan Qu, Lihui Liu, Xue Zhang, Yan He, Chao Wang, Yufeng Guo, Li Yuan, Zixiao Ma, Hua Bai, Jie Wang
Summary: This study investigated the plasma exosomes' proteomic profiles in patients with lung cancer (LC) who had liver metastasis (LM) or brain metastasis (BM). Differentially expressed exosome-based proteins (DEEPs) were identified in LM and BM, and they were found to be involved in proteomic triggering cascade, ECM-receptor interaction, and the collagen-containing extracellular matrix. Potential biomarkers for the diagnosis of LM and BM were also suggested.
CELL AND BIOSCIENCE
(2023)
Article
Dermatology
Ali Azimi, Ellis Patrick, Rachel Teh, Jennifer Kim, Pablo Fernandez-Penas
Summary: Despite advances in managing metastatic melanomas, patients' overall survival remains low. This study investigated the proteome-wide changes associated with melanoma metastasis using mass spectrometry-based proteomics and bioinformatics analysis. Protein signatures were identified that distinguished regional lymph node and distant organ metastatic melanomas from primary lesions, providing valuable information for potential targeted therapies.
EXPERIMENTAL DERMATOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Joohyun Ryu, Stefani N. Thomas
Summary: Ovarian cancer is a deadly gynecologic malignancy with the need for new and more effective treatment strategies, while early detection and protein biomarkers play crucial roles. Targeted proteomics methods, including mass spectrometry, offer robust solutions for the development of ovarian cancer biomarkers.
Article
Oncology
Dylan Dieters-Castator, Paola M. Dantonio, Matt Piaseczny, Guihua Zhang, Jiahui Liu, Miljan Kuljanin, Stephen Sherman, Michael Jewer, Katherine Quesnel, Eun Young Kang, Martin Koebel, Gabrielle M. Siegers, Andrew Leask, David Hess, Gilles Lajoie, Lynne-Marie Postovit
Summary: The embryonic secreted protein NODAL correlates with activated fibroblasts in triple-negative breast cancers and reprograms cancer cell secretomes, leading to changes in MSC chemotaxis, highlighting a previously unrecognized mechanism in the dynamic regulation of the tumor microenvironment.
Review
Pharmacology & Pharmacy
Qi Xiao, Fangfei Zhang, Luang Xu, Liang Yue, Oi Lian Kon, Yi Zhu, Tiannan Guo
Summary: This paper summarizes the advances and limitations of biomarkers in cancer research, as well as the important applications of mass spectrometry technology in proteomics. The article emphasizes the role of artificial intelligence technology in clinical studies, as well as the importance of combining computational and statistical methods in biomarker research.
ADVANCED DRUG DELIVERY REVIEWS
(2021)
Article
Biochemistry & Molecular Biology
Yewei Wang, Zeljka Popovic, Georgia Charkoftaki, Rolando Garcia-Milian, TuKiet T. Lam, David C. Thompson, Ying Chen, Vasilis Vasiliou
Summary: This study identifies the impact of knocking down ALDH1B1 on molecular signatures in colon cancer cells through multi-omics analysis. The findings provide new molecular information associated with the cellular functions of ALDH1B1, which will guide future investigations of colon cancer.
CHEMICO-BIOLOGICAL INTERACTIONS
(2023)
Article
Oncology
Yanhong Liu, Lan Gao, Yanru Fan, Rufei Ma, Yunxia An, Guanghui Chen, Yan Xie
Summary: Potential protein biomarkers and mechanism of venous thromboembolism (VTE) in non-small cell lung cancer (NSCLC) patients were identified through proteomics research. A total of 280 differentially expressed proteins were found, and 5 proteins (SAA1, S100A8, LBP, HP and LDHB) showed significant changes between VTE and non-VTE patients. These results suggest that these proteins may be used for diagnosing VTE in NSCLC patients.
FRONTIERS IN ONCOLOGY
(2023)