Phage-assisted evolution and protein engineering yield compact, efficient prime editors

Predicting prime editing efficiency and product purity by deep learning

(2023) Nicolas Mathis et al.   NATURE BIOTECHNOLOGY

Prediction of efficiencies for diverse prime editing systems in multiple cell types

(2023) Goosang Yu et al.   CELL

Prediction of prime editing insertion efficiencies using sequence features and DNA repair determinants

(2023) Jonas Koeppel et al.   NATURE BIOTECHNOLOGY

Engineered virus-like particles for efficient in vivo delivery of therapeutic proteins

(2022) Samagya Banskota et al.   CELL

AAV gene therapy for Tay-Sachs disease

(2022) Terence R. Flotte et al.   NATURE MEDICINE

Prime editing efficiency and fidelity are enhanced in the absence of mismatch repair

(2022) J. Ferreira da Silva et al.   Nature Communications

CRISPR-free base editors with enhanced activity and expanded targeting scope in mitochondrial and nuclear DNA

(2022) Beverly Y. Mok et al.   NATURE BIOTECHNOLOGY

A split prime editor with untethered reverse transcriptase and circular RNA template

(2022) Bin Liu et al.   NATURE BIOTECHNOLOGY

Author Correction: An engineered prime editor with enhanced editing efficiency in plants

(2022) Yuan Zong et al.   NATURE BIOTECHNOLOGY

An engineered prime editor with enhanced editing efficiency in plants

(2022) Yuan Zong et al.   NATURE BIOTECHNOLOGY

Efficient targeted insertion of large DNA fragments without DNA donors

(2022) Jinlin Wang et al.   NATURE METHODS

Enhancement of prime editing via xrRNA motif-joined pegRNA

(2022) Guiquan Zhang et al.   Nature Communications

Designing and executing prime editing experiments in mammalian cells

(2022) Jordan L. Doman et al.   Nature Protocols

Bi-PE: bi-directional priming improves CRISPR/Cas9 prime editing in mammalian cells

(2022) Rui Tao et al.   NUCLEIC ACIDS RESEARCH

Peptide fusion improves prime editing efficiency

(2022) Minja Velimirovic et al.   Nature Communications

High-throughput continuous evolution of compact Cas9 variants targeting single-nucleotide-pyrimidine PAMs

(2022) Tony P. Huang et al.   NATURE BIOTECHNOLOGY

Cryo-EM structures of Escherichia coli Ec86 retron complexes reveal architecture and defence mechanism

(2022) Yanjing Wang et al.   Nature Microbiology

Drag-and-drop genome insertion of large sequences without double-strand DNA cleavage using CRISPR-directed integrases

(2022) Matthew T. N. Yarnall et al.   NATURE BIOTECHNOLOGY

High-efficiency prime editing with optimized, paired pegRNAs in plants

(2021) Qiupeng Lin et al.   NATURE BIOTECHNOLOGY

Improved prime editors enable pathogenic allele correction and cancer modelling in adult mice

(2021) Pengpeng Liu et al.   Nature Communications

Five-Year Extension Results of the Phase 1 START Trial of Onasemnogene Abeparvovec in Spinal Muscular Atrophy

(2021) Jerry R. Mendell et al.   JAMA Neurology

Dual-AAV delivering split prime editor system for in vivo genome editing

(2021) Shengyao Zhi et al.   MOLECULAR THERAPY

Highly accurate protein structure prediction with AlphaFold

(2021) John Jumper et al.   NATURE

Enhanced prime editing systems by manipulating cellular determinants of editing outcomes

(2021) Peter J. Chen et al.   CELL

Deletion and replacement of long genomic sequences using prime editing

(2021) Tingting Jiang et al.   NATURE BIOTECHNOLOGY

Engineered pegRNAs improve prime editing efficiency

(2021) James W. Nelson et al.   NATURE BIOTECHNOLOGY

Precise genomic deletions using paired prime editing

(2021) Junhong Choi et al.   NATURE BIOTECHNOLOGY

Programmable deletion, replacement, integration and inversion of large DNA sequences with twin prime editing

(2021) Andrew V. Anzalone et al.   NATURE BIOTECHNOLOGY

Increasing the efficiency and precision of prime editing with guide RNA pairs

(2021) Yuan Zhuang et al.   Nature Chemical Biology

Cytosine and adenine base editing of the brain, liver, retina, heart and skeletal muscle of mice via adeno-associated viruses

(2020) Jonathan M. Levy et al.   Nature Biomedical Engineering

Prime genome editing in rice and wheat

(2020) Qiupeng Lin et al.   NATURE BIOTECHNOLOGY

Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity

(2020) Michelle F. Richter et al.   NATURE BIOTECHNOLOGY

CHANGE-seq reveals genetic and epigenetic effects on CRISPR–Cas9 genome-wide activity

(2020) Cicera R. Lazzarotto et al.   NATURE BIOTECHNOLOGY

DNA capture by a CRISPR-Cas9–guided adenine base editor

(2020) Audrone Lapinaite et al.   SCIENCE

Predicting the efficiency of prime editing guide RNAs in human cells

(2020) Hui Kwon Kim et al.   NATURE BIOTECHNOLOGY

Bacterial Retrons Function In Anti-Phage Defense

(2020) Adi Millman et al.   CELL

Phage-assisted continuous and non-continuous evolution

(2020) Shannon M. Miller et al.   Nature Protocols

Phage-Assisted Evolution of Bacillus methanolicus Methanol Dehydrogenase 2

(2019) Timothy B. Roth et al.   ACS Synthetic Biology

Challenges in the quest for ‘clean meat’

(2019) Lieven Thorrez et al.   NATURE BIOTECHNOLOGY

Continuous evolution of base editors with expanded target compatibility and improved activity

(2019) Benjamin W. Thuronyi et al.   NATURE BIOTECHNOLOGY

Search-and-replace genome editing without double-strand breaks or donor DNA

(2019) Andrew V. Anzalone et al.   NATURE

AmpUMI: design and analysis of unique molecular identifiers for deep amplicon sequencing

(2018) Kendell Clement et al.   BIOINFORMATICS

Structure of a Thermostable Group II Intron Reverse Transcriptase with Template-Primer and Its Functional and Evolutionary Implications

(2017) Jennifer L. Stamos et al.   MOLECULAR CELL

Enhanced proofreading governs CRISPR–Cas9 targeting accuracy

(2017) Janice S. Chen et al.   NATURE

Virus-Mediated Genome Editing via Homology-Directed Repair in Mitotic and Postmitotic Cells in Mammalian Brain

(2017) Jun Nishiyama et al.   NEURON

The initiation, propagation and dynamics of CRISPR-SpyCas9 R-loop complex

(2017) Yan Zeng et al.   NUCLEIC ACIDS RESEARCH

In vivo genome editing via CRISPR/Cas9 mediated homology-independent targeted integration

(2016) Keiichiro Suzuki et al.   NATURE

Continuous directed evolution of DNA-binding proteins to improve TALEN specificity

(2015) Basil P Hubbard et al.   NATURE METHODS

Rationally engineered Cas9 nucleases with improved specificity

(2015) I. M. Slaymaker et al.   SCIENCE

Development of potent in vivo mutagenesis plasmids with broad mutational spectra

(2015) Ahmed H. Badran et al.   Nature Communications

Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA

(2014) Hiroshi Nishimasu et al.   CELL

Structural basis of PAM-dependent target DNA recognition by the Cas9 endonuclease

(2014) Carolin Anders et al.   NATURE

Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression

(2013) Lei S. Qi et al.   CELL

Structural analysis of monomeric retroviral reverse transcriptase in complex with an RNA/DNA hybrid

(2013) Elżbieta Nowak et al.   NUCLEIC ACIDS RESEARCH

Experimental interrogation of the path dependence and stochasticity of protein evolution using phage-assisted continuous evolution

(2013) B. C. Dickinson et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Generation and characterization of new highly thermostable and processive M-MuLV reverse transcriptase variants

(2012) A. Baranauskas et al.   PROTEIN ENGINEERING DESIGN & SELECTION

A system for the continuous directed evolution of biomolecules

(2011) Kevin M. Esvelt et al.   NATURE

NUPACK: Analysis and design of nucleic acid systems

(2010) Joseph N. Zadeh et al.   JOURNAL OF COMPUTATIONAL CHEMISTRY

Efficient stable gene transfer into human cells by the Sleeping Beauty transposon vectors

(2009) Z IZSVAK   METHODS

Novel mutations in Moloney Murine Leukemia Virus reverse transcriptase increase thermostability through tighter binding to template-primer

(2008) Bahram Arezi et al.   NUCLEIC ACIDS RESEARCH

A One Pot, One Step, Precision Cloning Method with High Throughput Capability

(2008) Carola Engler et al.   PLoS One