Effect of Short PSG Peptides on Inflammatory Markers in Allogeneic Bone Marrow Cell Transplantation in Wistar Rats

Short linear peptide fragments of placental trophoblastic beta 1-glycoprotein (PSG) (YECE, YQCE, YVCS, and YACS) were studied in the context of their immunomodulatory effects at the level of inflammatory markers. The original host-versus-graft model was used in male Wistar rats without prior conditioning of recipient bone marrow. A composition of PSG peptide fragments was injected to animals after allogeneic transplantation of bone marrow cells in a dynamic experiment, inflammatory markers alpha 1-acid glycoprotein (AGP, orosomucoid), alpha 2-macroglobulin (alpha 2M) were assayed by ELISA, and biochemical parameters (total protein, glucose, creatinine, and urea) were measured. The levels of alpha 2M and AGP increased in response to allotransplantation, whereas administration of PSG peptides normalized serum alpha 2M levels by the end of the experiment. The decrease in alpha 2M level coincided with the independent effect of PSG peptide administration. The levels of total protein, glucose, creatinine, and urea in rat serum after allotransplantation were reduced throughout the experiment. Administration of PSG peptides contributed to normalization of serum total protein, creatinine, and urea levels by the end of the experiment. Administration of PSG peptides after allogeneic transplantation of bone marrow suspension contributed to normalization of the levels of alpha 2M, total protein, creatinine, and urea, which can be interpreted as an anti-inflammatory effect of these peptides.

Automated summary

Short linear peptide fragments of placental trophoblastic beta 1-glycoprotein (PSG) exhibit anti-inflammatory effects by modulating inflammatory markers in the context of bone marrow transplantation.