Angiogenin as a Possible Mediator of Macrophage-Mediated Regulation of Fibroblast Functions

We studied angiogenin production by human macrophages and evaluated the role of this factor in the macrophage-mediated regulation of fibroblasts. All macrophage subtypes, and especially the efferocytosis-polarized macrophages, M2(LS), actively produced angiogenin. Exogenous recombinant angiogenin dose-dependently enhanced the proliferation and differentiation of dermal fibroblasts. The addition of the angiogenin inhibitor to fibroblasts cultures suppressed the stimulating effect of exogenous angiogenin or M2(LS) conditioned media. These findings indicate the involvement of angiogenin in the macrophage-mediated paracrine regulation of skin fibroblasts.

Automated summary

This study investigated the production of angiogenin by human macrophages and its role in the regulation of fibroblasts. It was found that all macrophage subtypes, particularly the efferocytosis-polarized macrophages, actively produced angiogenin. Exogenous angiogenin significantly promoted the proliferation and differentiation of dermal fibroblasts. Furthermore, the addition of an angiogenin inhibitor suppressed the stimulating effect of exogenous angiogenin or M2(LS) conditioned media on fibroblasts, indicating the involvement of angiogenin in macrophage-mediated paracrine regulation of skin fibroblasts.