期刊
BIOTECHNIQUES
卷 75, 期 5, 页码 211-218出版社
FUTURE SCI LTD
DOI: 10.2144/btn-2023-0030
关键词
colorimetric; G-quadruplex; postpartum care infection; Pseudomonas aeruginosa; P. aeruginosa
A simple, sensitive, and colorimetric assay for Pseudomonas aeruginosa detection is described in this study. The approach exhibits a wide detection range and a low limit of detection, which may assist in the early diagnosis of postpartum care skin infections.
Skin and soft tissue infections caused by Pseudomonas aeruginosa are common acquired diseases in postpartum care. Many methods have been developed in recent years for detecting P. aeruginosa, but they are criticized for the drawbacks of labor-intensiveness, complicated operation and high cost. Here, a simple, sensitive and colorimetric assay for P. aeruginosa detection is described. The approach displays a green color for positive samples and colorless for target-free samples. The approach exhibits a wide detection range and a low limit of detection of 45 CFU/ml. Thus, the developed ligation-initiated multiple-signal amplification method may be used for on-site testing of pathogenic bacteria and assist in the early diagnosis of postpartum care skin infections.
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