期刊
MEDICAL AND VETERINARY ENTOMOLOGY
卷 30, 期 2, 页码 155-165出版社
WILEY
DOI: 10.1111/mve.12163
关键词
Culicoides; bloodmeal; host identification; midge; real-time PCR
资金
- New South Wales Department of Primary Industries
- University of Sydney
Culicoides (Diptera: Ceratopogonidae) midges are the biological vectors of a number of arboviruses of veterinary importance. However, knowledge relating to the basic biology of some species, including their host-feeding preferences, is limited. Identification of host-feeding preferences in haematophagous insects can help to elucidate the transmission dynamics of the arboviruses they may transmit. In this study, a series of semi-quantitative real-time polymerase chain reaction (qPCR) assays to identify the vertebrate host sources of bloodmeals of Culicoides midges was developed. Two pan-reactive species group and seven species-specific qPCR assays were developed and evaluated. The assays are quick to perform and less expensive than nucleic acid sequencing of bloodmeals. Using these assays, it was possible to rapidly test nearly 700 blood-fed midges of various species from several geographic locations in Australia.
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