Article
Biochemistry & Molecular Biology
Emirhan Nemutlu, Fatih Ozaltin, Samiye Yabanoglu-Ciftci, Bora Gulhan, Cemil Can Eylem, Ipek Baysal, Elif Damla Gok-Topak, Kezban Ulubayram, Osman Ugur Sezerman, Gulberk Ucar, Sedef Kir, Rezan Topaloglu
Summary: This study identified candidate biomarkers for the diagnosis and follow-up of cystinosis through multiomics studies. 10 metabolites were found to differ between the patient and control groups in plasma metabolomics analysis, and several metabolites were also significantly different in urine metabolomic analysis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Environmental Sciences
Hui Jiao, Tingting Yuan, Xiaohuan Wang, Xia Zhou, Renyue Ming, Honghao Cui, Deyu Hu, Ping Lu
Summary: This study assessed the toxic effects of acetamiprid on Xenopus laevis and found that it had detrimental effects on the liver, causing oxidative stress and histological damage. Metabolomic analysis revealed disrupted metabolic pathways, indicating the hepatotoxic effects of acetamiprid on X. laevis at a molecular level. These findings are crucial for evaluating the aquatic risks of neonicotinoids.
ENVIRONMENTAL POLLUTION
(2023)
Article
Oncology
Ming Yang, Xiaoyan Liu, Xiaoyue Tang, Wei Sun, Zhigang Ji
Summary: Urine metabolomics is a promising technique in the liquid biopsy of urothelial cancer (UC). In this study, urine samples from UTUCs, BCas, and HCs were analyzed using LC-HRMS, and differential metabolites and disturbed metabolism pathways were explored. The combination of urine metabolomics and transcriptomics identified potential aberrant genes in BCa metabolism.
FRONTIERS IN ONCOLOGY
(2023)
Article
Medicine, General & Internal
Ning Liu, Jing Xiao, Charul Gijavanekar, Kirk L. Pappan, Kevin E. Glinton, Brian J. Shayota, Adam D. Kennedy, Qin Sun, V. Reid Sutton, Sarah H. Elsea
Summary: The study compared the diagnostic rates of traditional metabolic screening and clinical metabolomics, finding that clinical metabolomics had a higher diagnostic rate and could identify a wider variety of inborn errors of metabolism.
Article
Biochemical Research Methods
Souhila Messaili, Yanyan Qu, Laetitia Fougere, Cyril Colas, Nicolas Desneux, Anne-Violette Lavoir, Emilie Destandau, Thomas Michel
Summary: The study aimed to investigate the phytochemical variations in tomato roots at two different development stages using an untargeted UHPLC-HRMS metabolomic approach. The main biomarkers identified were guanidine and adenosine derivatives associated with tryptophan, as well as secondary metabolites such as glycoalkaloids and steroidal alkaloids. Metabolites were found to be up-regulated in young tomato roots, while tryptophan was up-regulated in older roots, contributing to a deeper understanding of early-stage root development and tomato root defence mechanisms.
PHYTOCHEMICAL ANALYSIS
(2021)
Article
Plant Sciences
Shengrui Yao, Dikshya Sapkota, Jordan A. A. Hungerford, Roland D. D. Kersten
Summary: This study reveals the metabolic differences between jujube fruits from different cultivars and growth sites using metabolomics. The results show that the cultivar is the primary factor influencing metabolite profiles, while the location is secondary. Furthermore, specific metabolites vary significantly among different cultivars. This research provides valuable guidance for selecting jujube cultivars with nutritional and medicinal value and for fruit metabolic breeding.
Article
Biochemistry & Molecular Biology
Cindy Arevalo, Laura Rojas, Mary Santamaria, Luisana Molina, Lina Arbelaez, Paula Sanchez, Ricardo Ballesteros-Ramirez, Monica Arevalo-Zambrano, Sandra Quijano, Monica P. Cala, Susana Fiorentino
Summary: This study conducted a multiplatform untargeted metabolomic and lipidomic approach to identify metabolic differences between acute leukemia patients and healthy individuals. Glycerolipid and sphingolipid metabolic pathways were the most affected, and the patients showed a decrease in total antioxidant capacity. The findings are consistent with previous research and suggest that the metabolic differences are independent of lifestyle, race, or geographic location.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Article
Plant Sciences
Stephanie Boutet, Lea Barreda, Francois Perreau, Jean-Chrisologue Totozafy, Caroline Mauve, Bertrand Gakiere, Etienne Delannoy, Marie-Laure Martin-Magniette, Andrea Monti, Loic Lepiniec, Federica Zanetti, Massimiliano Corso
Summary: This study characterizes the landscape of specialized metabolites (SMs), primary metabolites, and lipid composition in camelina seeds grown in the open field. The predominant effect of the environment on seed SM regulation is observed, which may have potential implications for seed quality in other oilseed crops.
Article
Biochemistry & Molecular Biology
Anna Piasecka, Aneta Sawikowska, Nicolas Jedrzejczak-Rey, Mariola Pislewska-Bednarek, Pawel Bednarek
Summary: Brachypodium distachyon, a model grass species, has been extensively studied due to its fully sequenced genome. However, its metabolome, an essential component of complex biological systems, remains poorly characterized. In this study, we conducted a comprehensive metabolomic examination using LC-MS technology to analyze the metabolite profiles of roots, leaves, and spikes of this species. Our results revealed the accumulation of specialized metabolites related to various metabolic pathways, including flavonoid biosynthesis, nucleotide metabolism, sugar metabolism, and amino acid metabolism. Structural analysis based on MS fragmentation spectra identified 93 specialized metabolites, mainly phenylpropanoids and flavonoid derivatives. These findings provide insights into the metabolic profile of Brachypodium and highlight the differences in metabolite composition among different plant tissues and lines.
Article
Chemistry, Multidisciplinary
Matthew T. Welling, Myrna A. Deseo, Antony Bacic, Monika S. Doblin
Summary: This study compares the metabolomes of male and female cannabis plants, revealing higher levels of phytocannabinoids in female plants, which have implications for sex expression and secondary metabolism in cannabis.
AUSTRALIAN JOURNAL OF CHEMISTRY
(2021)
Article
Oncology
Seungyoun Jung, Sarah Silva, Cher M. Dallal, Erin Leblanc, Kenneth Paris, John Shepherd, Linda G. Snetselaar, Linda Van Horn, Yuji Zhang, Joanne F. Dorgan
Summary: This study aimed to investigate the metabolic influences on breast density phenotypes. Serum metabolomics analyses identified metabolic signatures associated with breast density phenotypes among young women. The results showed an inverse association of branched chain amino acids with breast density.
CANCER CAUSES & CONTROL
(2023)
Article
Biochemistry & Molecular Biology
Julia E. Stroud, Michael S. Gale, Sara R. Zwart, Martina Heer, Scott M. Smith, Tony Montina, Gerlinde A. S. Metz
Summary: This study investigated the longitudinal metabolic changes of male and female astronauts during long-duration missions to the International Space Station using a metabolomics-based approach. The research found that space travel resulted in sex-specific changes in energy metabolism, bone and muscle regulation, immunity, and macromolecule synthesis. Furthermore, metabolic signatures suggest that females require more time to adjust to returning to Earth.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Plant Sciences
Xinxin Zhang, Yuxi Li, Huiling Yan, Kewei Cai, Hanxi Li, Zhiwei Wu, Jianguo Wu, Xiangdong Yang, Haichen Jiang, Qingcheng Wang, Guanzheng Qu, Xiyang Zhao
Summary: Light is a vital energy source and regulator for plant growth and development. The mechanism of plant growth and development under low light conditions is a significant scientific question. This study measured the growth and physiological indexes of Juglans mandshurica under different shade treatments and analyzed the molecular mechanism and metabolite accumulation under light stress. The results showed that seedling height was significantly higher in the shade treatment group, especially with 50% light intensity. The transcriptome and metabolome analyses revealed differential gene expression and metabolite accumulation related to photosynthesis, plant hormone signal transduction, and chlorophyll synthesis pathways. Flavonoids were identified as the major differential metabolites under light stress. These findings enhance our understanding of the molecular mechanisms and metabolite accumulation under light stress in J. mandshurica.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Microbiology
Jacob J. J. Haffner, Mitchelle Katemauswa, Therese S. Kagone, Ekram Hossain, David Jacobson, Karina Flores, Adwaita R. R. Parab, Alexandra J. J. Obregon-Tito, Raul Y. Y. Tito, Luis Marin Reyes, Luzmila Troncoso-Corzo, Emilio Guija-Poma, Nicolas Meda, Helene Carabin, Tanvi P. P. Honap, Krithivasan Sankaranarayanan, Cecil M. M. Lewis, Laura-Isobel McCall
Summary: As industrialization progresses, understanding the biological consequences of lifestyle shifts and their impact on human health is crucial. This study characterizes the shared human fecal metabolome across populations from Africa and the Americas, highlighting the influence of industrialization on this metabolome. The findings demonstrate that industrialization significantly affects the human fecal metabolome, but there are still shared features among diverse human lifestyles.
Article
Food Science & Technology
Yueqi Wang, Shi Nie, Chunsheng Li, Huan Xiang, Yongqiang Zhao, Shengjun Chen, Laihao Li, Yanyan Wu
Summary: This study characterized the taste-related metabolite profiles during the fermentation of mandarin fish using untargeted metabolomics. It identified amino acids as significant contributors to the taste quality and revealed amino acid metabolism as the dominant pathway in the fermentation process.