Article
Chemistry, Physical
Yilun Weng, Andria Wan, Yang Li, Yun Liu, Xiaojing Chen, Hao Song, Chun-Xia Zhao
Summary: Enzymes are important for their efficiency, selectivity, and environmental sustainability in various industries. However, their low thermal stability hampers their application. To overcome this, we developed an encapsulation technology using alginate-enzyme colloidal system, which showed excellent thermal stability during a model pelleting process. We also developed a lab-scale method to evaluate enzyme thermal stability, saving time and cost. The scalable production of enzyme loaded alginate particles with high thermal and storage stability is an effective strategy.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2023)
Article
Chemistry, Applied
Yilun Weng, Supun Ranaweera, Da Zou, Anna P. Cameron, Xiaojing Chen, Hao Song, Chun-Xia Zhao
Summary: Enzymes are essential for industries, but their sensitivity to high temperatures can be problematic. Encapsulation with alginate microparticles has been shown to improve enzyme thermal stability, but other factors like enzyme loading and bioavailability also need to be considered. This study optimized the efficiency of enzyme encapsulation and achieved high enzyme loading and thermal stability, with excellent bioavailability demonstrated in an animal trial.
FOOD HYDROCOLLOIDS
(2023)
Article
Chemistry, Physical
Sara Hedayati, Mohammad Tarahi, Rezvan Azizi, Vahid Baeghbali, Elham Ansarifar, Mohammad Hashem Hashempur
Summary: Mint essential oil (MEO) is a natural substance with antibacterial and antioxidant properties, but its low solubility and volatility limit its applications. Encapsulation technology has emerged as a promising method to improve the stability of MEO and expand its uses in various industries.
ADVANCES IN COLLOID AND INTERFACE SCIENCE
(2023)
Review
Polymer Science
Milan Milivojevic, Aleksandra Popovic, Ivana Pajic-Lijakovic, Ivan Sostaric, Stefan Kolasinac, Zora Dajic Stevanovic
Summary: This article systematically reviews the properties of sodium alginate, emphasizing its important role in gel formation with divalent cations. The abundant, low-cost, eco-friendly, bio-compatible, bio-adhesive, biodegradable, and stable nature of sodium alginate is also discussed. The article explores the use of alginate-based encapsulation systems in improving the stability and bioavailability of carotenoids, indicating that micro and nano-sized, as well as emulsion-based systems, have the potential to enhance their low bioaccessibility. Furthermore, the incorporation of other biopolymers can further improve the properties of the encapsulation system.
Article
Biochemistry & Molecular Biology
Bogumil Brycki, Adrianna Szulc, Iwona Kowalczyk, Justyna Brycka
Summary: This study demonstrates the efficient preparation methods of encapsulated gemini surfactants using alginate and gelatin capsules. The effectiveness of the encapsulation is confirmed through infrared spectra analysis and scanning electron microscope imaging. Gemini surfactants in encapsulated form show promising potential as corrosion inhibitors and antimicrobials, with the ability to protect the active substance and control release.
Review
Food Science & Technology
Chanez Bennacef, Stephane Desobry, Laurent Probst, Sylvie Desobry-Banon
Summary: Encapsulation is a technology used in various industries to protect active molecules and control their release. Coextrusion encapsulation methods can produce core-shell capsules. This review focuses on the applications of coextrusion encapsulation for preserving oils, essential oils, probiotics, and other bioactives. This technology isolates the active molecules, enhances their stability, and allows controlled release. Coextrusion offers valuable opportunities for preserving active molecules in pharmaceutical, food, and agriculture sectors.
Review
Polymer Science
Anna Letocha, Malgorzata Miastkowska, Elzbieta Sikora
Summary: This article introduces the wide application of alginates in the pharmaceutical, food, and cosmetic industries as natural polymers used as thickening, gel-forming, and stabilizing agents. Alginate microparticles (AMP) are biocompatible, biodegradable, and non-toxic carriers used for encapsulating hydrophilic active substances. The article discusses the methods, technological parameters, advantages, and disadvantages of AMP production and its role as a delivery system.
Article
Materials Science, Multidisciplinary
Daniel Massana Roquero, Paolo Bollella, Evgeny Katz, Artem Melman
Summary: The high porosity of calcium alginate hydrogels can be controlled by treatment with polyvinyl alcohol and cross-linking with diboronic acids, effectively blocking larger pores. This process reduces leaching of proteins trapped in the hydrogels by 20-30 fold, confirmed by microscopy techniques.
ACS APPLIED POLYMER MATERIALS
(2021)
Article
Chemistry, Applied
Luping Gu, David Julian McClements, Jiaying Li, Yujie Su, Yanjun Yang, Junhua Li
Summary: In this study, alginate/kappa-carrageenan composite beads were developed for encapsulation and release of egg yolk immunoglobulin Y (IgY) under simulated gastrointestinal conditions. The composite beads showed higher efficiency in protecting IgY activity and slower release kinetics compared to pure alginate beads, making them promising carriers for hydrophilic immunoglobins.
FOOD HYDROCOLLOIDS
(2021)
Review
Chemistry, Multidisciplinary
Shuke Wu, Radka Snajdrova, Jeffrey C. Moore, Kai Baldenius, Uwe T. Bornscheuer
Summary: Biocatalysis is widely used as an alternative to chemical catalysis in various fields, with enzymes playing a key role in organic synthesis, particularly in the production of chiral compounds for pharmaceuticals and flavors. Biocatalysts are also utilized on a large scale for the production of specialty and bulk chemicals. This review focuses on scalable chemical production using enzymes, discussing opportunities, limitations, and emerging enzyme classes through illustrative examples.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Biochemistry & Molecular Biology
Marina Paiva Abucafy, Regina Celia Galvao Frem, Giulia Polinario, Fernando Rogerio Pavan, Heng Zhao, Angelika Mielcarek, Cedric Boissiere, Christian Serre, Leila Aparecida Chiavacci
Summary: This study develops porous capsules from metal-organic framework nanoparticles (MOF NPs), which can encapsulate an antitumor drug and collagenase enzyme for enhanced tumor treatment. The capsules show better control of drug release and higher toxicity against cancer cells compared to bare MOF NPs. This research has important implications for the development of dual drug delivery systems for cancer treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Nanoscience & Nanotechnology
Ishtpreet Kaur, Shekhar Agnihotri, Dinesh Goyal
Summary: In this study, insecticide cartap hydrochloride was fabricated as nanospheres using a two-step method. The nanospheres showed good stability and encapsulation efficiency. The release experiments indicated that the nanospheres had controlled release properties.
Article
Food Science & Technology
Xiaohan Ge, Yuting Sun, Jianglong Kong, Meiru Mao, Hongrui Yu, Amit Arora, Inthawoot Suppavorasatit, Yi Wang
Summary: This study successfully fabricated a zein-sodium alginate binary nanoparticle system loaded with riboflavin using the anti-solvent precipitation method, which improved the delivery efficiency and stability of riboflavin in the gastrointestinal tract.
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
(2023)
Article
Food Science & Technology
Chun-Ru Su, Yu-Yan Huang, Qi-Hui Chen, Meng-Fan Li, Hao Wang, Guo-Yan Li, Yang Yuan
Summary: In this study, a novel complex coacervate between gliadin and sodium alginate was formed and successfully loaded with curcumin, demonstrating spherical particles with good homogeneity and dispersion. The formation of coacervates at different pH induced by electrostatic interaction reduced the release speed of curcumin, leading to better encapsulation and controlled release properties.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Food Science & Technology
Jian Gao, Chengzhi Liu, Jieyu Shi, Fangfang Ni, Qing Shen, Hujun Xie, Kuiwu Wang, Qunfang Lei, Wenjun Fang, Gerui Ren
Summary: The novel quaternary protein polysaccharide complexes, containing OVA, PEC, SA, and VD3, showed excellent stability and sustained-release performance for VD3, with good encapsulation efficiency and loading capacity. These complex nanoparticles have potential applications in the food and drug industry due to their stability and controlled release properties for vitamin D.
FOOD RESEARCH INTERNATIONAL
(2021)
Article
Agriculture, Multidisciplinary
Yilun Weng, Supun Ranaweera, Da Zou, Anna Cameron, Xiaojing Chen, Hao Song, Chun-Xia Zhao
Summary: This study demonstrates a simple and effective method for enzyme immobilization via spray drying using alginate as a support material. The immobilized enzyme retains a high level of activity at high temperatures and can be promptly released in a simulated gastrointestinal tract.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Chemistry, Physical
Yilun Weng, Andria Wan, Yang Li, Yun Liu, Xiaojing Chen, Hao Song, Chun-Xia Zhao
Summary: Enzymes are important for their efficiency, selectivity, and environmental sustainability in various industries. However, their low thermal stability hampers their application. To overcome this, we developed an encapsulation technology using alginate-enzyme colloidal system, which showed excellent thermal stability during a model pelleting process. We also developed a lab-scale method to evaluate enzyme thermal stability, saving time and cost. The scalable production of enzyme loaded alginate particles with high thermal and storage stability is an effective strategy.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2023)
Review
Food Science & Technology
Yilun Weng, Yang Li, Xiaojing Chen, Hao Song, Chun-Xia Zhao
Summary: Enzymes are commonly used in the food industry to improve the properties of food products, but their poor stability and limited shelf-lives have hindered their applications. This review focuses on the use of spray drying as a promising method for enzyme encapsulation in the food industry. Recent studies and achievements in enzyme encapsulation using spray drying are summarized, along with the latest developments in spray drying techniques. The scale-up pathways from laboratory trials to industrial productions are also discussed.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Chemistry, Applied
Yilun Weng, Supun Ranaweera, Da Zou, Anna P. Cameron, Xiaojing Chen, Hao Song, Chun-Xia Zhao
Summary: Enzymes are essential for industries, but their sensitivity to high temperatures can be problematic. Encapsulation with alginate microparticles has been shown to improve enzyme thermal stability, but other factors like enzyme loading and bioavailability also need to be considered. This study optimized the efficiency of enzyme encapsulation and achieved high enzyme loading and thermal stability, with excellent bioavailability demonstrated in an animal trial.
FOOD HYDROCOLLOIDS
(2023)
Article
Biotechnology & Applied Microbiology
Carlos H. H. Luna-Flores, Yilun Weng, Alexander Wang, Xiaojing Chen, Bingyin Peng, Chun-Xia Zhao, Laura Navone, Juhani von Hellens, Robert E. E. Speight
Summary: This study aims to optimize the expression of plant acid phosphatase in P. pastoris by using continuous and fed-batch cultures in an industrial fermentation apparatus. The results showed that higher enzyme production can be achieved through continuous culture under glucose de-repression conditions. Furthermore, enzyme production was further improved by introducing methanol induction in fed-batch cultures. This study highlights the importance of optimizing protein production in P. pastoris.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Article
Engineering, Environmental
Penghui Yan, Idris Nur Azreena, Hong Peng, Hesamoddin Rabiee, Mohamed Ahmed, Yilun Weng, Zhonghua Zhu, Eric M. Kennedy, Michael Stockenhuber
Summary: Catalytic hydropyrolysis with zeolite catalysts improves the yield of liquid oil and gas products and reduces the production of biochar. The biochar produced from catalytic hydropyrolysis has a higher surface area compared to non-catalytic biochar.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Chemistry, Physical
Yang Li, Guangze Yang, Yilun Weng, Letao Xu, Fei Hou, Shankar Devkota, Chun-Xia Zhao
Summary: Surfactants are important in various applications, but traditional chemical surfactants have environmental and health risks. Bio-inspired surfactants are promising alternatives due to their biodegradability, biocompatibility, and adjustable functionality.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2023)
Article
Chemistry, Multidisciplinary
Letao Xu, Xing Wang, Guangze Yang, Zihan Zhao, Yilun Weng, Yang Li, Yun Liu, Chun-Xia Zhao
Summary: Lipid-based nanostructures have gained significant attention for their clinical applications, including the approval of the liposome Doxil for cancer therapy in 1995 and the recent mRNA lipid nanoparticle vaccines for COVID-19. Compared to liposomes, lipid nanoparticles with a particle structure offer advantages such as greater stability and higher drug encapsulation efficiency. This study introduces a concentration-controlled nanoprecipitation platform technology to fabricate lipid nanoparticles with tunable drug loading up to 70 wt%. The resulting nanoparticles exhibit excellent properties and hold promise for future drug delivery applications.
