Article
Engineering, Biomedical
Jiang-Shan Gong, Guo-Qiang Zhu, Yu Zhang, Bei Chen, Yi-Wei Liu, Hong-Ming Li, Ze-Hui He, Jing-Tao Zou, Yu-Xuan Qian, Sheng Zhu, Xin-Yue Hu, Shan-Shan Rao, Jia Cao, Hui Xie, Zhen-Xing Wang, Wei Du
Summary: This study developed a novel BMSC-aptamer functionalized hydrogel and demonstrated its effectiveness in recruiting BMSCs and promoting bone regeneration.
MATERIALS TODAY BIO
(2023)
Article
Orthopedics
Zhuo Chen, Mingchao Jin, Hongyi He, Jiabao Dong, Jing Li, Jiangbo Nie, Zichen Wang, Juntao Xu, Fengfeng Wu
Summary: Tendon-bone insertion injuries (TBI) such as ACL and rotator cuff injuries have a negative impact on daily life and cause economic losses. The healing process is complex and involves macrophages and MSCs. MSCs respond to inflammation and can differentiate into different tissues, promoting the reconstruction of the enthesis. This review discusses the roles of macrophages and MSCs in TBI injury and healing.
JOURNAL OF ORTHOPAEDIC TRANSLATION
(2023)
Review
Biochemistry & Molecular Biology
Qing Zhao, Xinran Liu, Chuanying Yu, Yu Xiao
Summary: This review provides a deep insight into the synergistic performance of BMSCs and macrophages in fracture healing, discussing their respective functions, relevant impact factors, and mechanisms. It aims to offer valuable cues and technical support for research on macrophage- and BMSC-related treatments, such as combined cell therapy for bone fractures.
DNA AND CELL BIOLOGY
(2022)
Article
Orthopedics
Yonghui Hou, Weiping Lin, Ying Li, Yuxin Sun, Yamei Liu, Chen Chen, Xiaohua Jiang, Gang Li, Liangliang Xu
Summary: The study showed that mir-92b could promote osteogenesis of MSCs and accelerate fracture healing. In vivo experiments confirmed the potential of De-Os-MSCs in bone regeneration.
JOURNAL OF ORTHOPAEDIC TRANSLATION
(2021)
Article
Computer Science, Interdisciplinary Applications
Enhao Zhang, Saeed Miramini, Lihai Zhang
Summary: This study investigates the combined effects of osteoporosis and diabetes on fracture healing process by developing numerical models. The results show that osteoporotic fractures have higher instability and disruption in mesenchymal stem cells' proliferation and differentiation compared to non-osteoporotic fractures. Moreover, when osteoporosis coexists with diabetes, the healing process of fractures can be severely impaired.
COMPUTER METHODS AND PROGRAMS IN BIOMEDICINE
(2024)
Article
Computer Science, Interdisciplinary Applications
Enhao Zhang, Saeed Miramini, Minoo Patel, Martin Richardson, Peter Ebeling, Lihai Zhang
Summary: The study found that there is a significant increase in TNF-alpha level in the fracture callus within the first day post-fracture under normal conditions, and its influence on the concentration of MSCs and cell differentiation becomes significant three days post-fracture. Excessive TNF-alpha secretion induced by diabetes could decrease the concentration of MSCs in the initial stage of healing.
COMPUTER METHODS AND PROGRAMS IN BIOMEDICINE
(2022)
Article
Agriculture, Dairy & Animal Science
Elena I. Pappa, Mariana S. Barbagianni, Stefanos G. Georgiou, Labrini V. Athanasiou, Dimitra Psalla, Dionysios Vekios, Eleni I. Katsarou, Natalia G. C. Vasileiou, Pagona G. Gouletsou, Apostolos D. Galatos, Nikitas N. Prassinos, Dimitris A. Gougoulis, Marianna Angelidou, Vicky Tsioli, George C. Fthenakis, Aikaterini I. Sideri
Summary: This study evaluated the efficacy of using autologous stromal vascular fraction from adipose tissue in enhancing bone healing. The findings suggest that the use of stromal vascular fraction can accelerate post-operative rehabilitation and significantly enhance bone formation, with osteogenic potential comparable to autologous bone graft.
Article
Biology
Dominik Saul, David G. Monroe, Jennifer L. Rowsey, Robyn Laura Kosinsky, Stephanie J. Vos, Madison L. Doolittle, Joshua N. Farr, Sundeep Khosla
Summary: Senescent cells and senescence-related secretory phenotype markers increase during fracture healing. Intermittent treatment with senolytics reduces senescence markers in the fracture callus and accelerates the time course of fracture healing.
Article
Pharmacology & Pharmacy
Xiao Yang, Jiang Shao, Xiao-Min Wu, Fei-Fei Pan, Shao-An Yang, Xiao-Hua Pan, An-Min Jin
Summary: Troxerutin, a semi-synthetic derivative of rutin, has been shown to promote osteogenesis in vitro and facilitate fracture healing in vivo, making it a promising therapeutic candidate for bone fracture repair.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Pharmacology & Pharmacy
Jian Dang, Wenjie Ling
Summary: This study demonstrated that MSCs can significantly improve bone mineral density in stress-fractured white rabbits, promote the release of bone formation factors, and aid in fracture healing, providing valuable guidance for clinical treatment of stress fractures.
PAKISTAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2021)
Article
Orthopedics
Fuxiao Wang, Jiawei Guo, Yili Wang, Yan Hu, Hao Zhang, Jiao Chen, Yingying Jing, Liehu Cao, Xiao Chen, Jiacan Su
Summary: This study found that Bcl-3 is closely related to bone fracture healing, and the loss of Bcl-3 delays bone fracture healing. Experimental evidence also demonstrated that Bcl-3 knockdown can inhibit the osteogenic differentiation of MSCs by promoting PP65 nuclear translocation.
JOURNAL OF ORTHOPAEDIC TRANSLATION
(2022)
Article
Biochemistry & Molecular Biology
Cui Zhang, Shali Wu, Erman Chen, Luyang Yu, Jinfu Wang, Mengrui Wu
Summary: This study reveals that the lncRNA AC132217.4 plays a critical role in BMSC osteogenesis, promoting the development and function of osteoblasts. AC132217.4 interacts with IGF2 mRNA to regulate its expression and activate the AKT pathway, thereby controlling osteoblast maturation and function. Additionally, the splicing factors SC35 and HNRNPA1 are involved in the biogenesis of AC132217.4, while the transcription factor ALX1 regulates the expression of AC132217.7 to promote osteogenesis. In vivo over-expression of AC132217.4 significantly promotes bone healing in mice.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Cell Biology
Takeshi Utsunomiya, Ning Zhang, Tzuhua Lin, Yusuke Kohno, Masaya Ueno, Masahiro Maruyama, Claire Rhee, Ejun Huang, Zhenyu Yao, Stuart B. Goodman
Summary: The local injection of MSCs or pMSCs during the chronic inflammatory phase can reverse decreased bone mineral density and increased osteoclast-like cells, while immediate injection of pMSCs during the acute inflammatory phase may impair bone healing. Timing of interventions to modulate inflammation is critical for bone healing outcomes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Yijun Wang, Wentao Chen, Liang Zhao, Yadong Li, Zhenyu Liu, Hua Gao, Xiaodong Bai, Baojun Wang
Summary: This study found that high-fat or obesity factors can inhibit the secretion of bone marrow stem cell-derived exosomes and affect the expression of osteogenic markers, leading to abnormal fracture healing. Treatment with exosomes from normal sources can reverse this phenomenon and improve fracture healing caused by obesity.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2021)
Review
Endocrinology & Metabolism
Xiaofeng Wang, Fanyuan Yu, Ling Ye
Summary: Recent studies have shown that MSCs play a vital role in bone regeneration, including osteogenic differentiation and providing seed cells. However, factors like drug intake and body senescence can affect MSCs' functions in bone regeneration. Epigenetic regulation has received attention for its heritable nature and involvement in bone metabolism disorders. This review focuses on the effects of epigenetic regulation on osteogenic differentiation, proliferation, and cellular senescence in MSCs and its role in bone regeneration.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Multidisciplinary Sciences
Keita Funada, Keigo Yoshizaki, Kanako MIyazaki, Xue Han, Tomomi Yuta, Tian Tian, Kanji Mizuta, Yao Fu, Tsutomu Iwamoto, Aya Yamada, Ichiro Takahashi, Satoshi Fukumoto
SCIENTIFIC REPORTS
(2020)
Article
Cell Biology
Tomoaki Nakamura, Tsutomu Iwamoto, Hannah M. Nakamura, Yuki Shindo, Kan Saito, Aya Yamada, Yoshihiko Yamada, Satoshi Fukumoto, Takashi Nakamura
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2020)
Review
Biochemistry & Molecular Biology
Keiji Masuda, Xu Han, Hiroki Kato, Hiroshi Sato, Yu Zhang, Xiao Sun, Yuta Hirofuji, Haruyoshi Yamaza, Aya Yamada, Satoshi Fukumoto
Summary: Dental pulp stem cells, derived from multipotent neural crest cells, have high proliferative capacity and multipotent potential for various applications. Stem cells donated by patients with genetic disorders can serve as disease models. Ethical considerations and potential applications of these stem cells donated and reused are worth exploring.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Shahad Al Thamin, Yuta Chiba, Keigo Yoshizaki, Tian Tian, LingLing Jia, Xin Wang, Kan Saito, Jiyao Li, Aya Yamada, Satoshi Fukumoto
Summary: Epithelial-mesenchymal interactions are crucial for tooth development, with growth factors playing critical roles in cellular differentiation. TGF-beta 1 was found to induce AmeloD expression and ameloblast differentiation through ERK1/2 and Smad2/3 phosphorylation mechanisms. These findings provide new insights into the regulatory mechanisms of tooth development.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Dentistry, Oral Surgery & Medicine
W. Ma, H. Lyu, M. Pandya, G. Gopinathan, X. Luan, T. G. H. Diekwisch
Summary: The nervous system plays a crucial role in controlling various aspects of organism life, with recent tissue regeneration research focusing on utilizing small molecule peptides to enhance biological responses. A study introduced the small molecule peptide galanin as a scaffold-coating agent for craniofacial tissue healing and regeneration, showing promising results in promoting cell proliferation, matrix synthesis, and mineralized tissue deposits. This suggests a potential novel role for neurogenic signaling pathways in craniofacial and periodontal regeneration.
JOURNAL OF DENTAL RESEARCH
(2021)
Article
Cell & Tissue Engineering
Mirali Pandya, Huling Lyu, Xianghong Luan, Thomas G. H. Diekwisch
Summary: The study demonstrates that coculturing cervical loop cells with dental pulp cells on tailored collagen scaffolds enriched with LRAP and early enamel matrix can result in singular, elongated, and polarized ameloblast-like cells expressing and secreting enamel proteins. Bioreactor microenvironments enriched with enamel matrix and LRAP are advantageous for the propagation of HAT-7 cells, leading to a significant increase in expression of amelogenin and ameloblastin enamel proteins. The use of microgravity culture systems and specific microenvironments along with tailored scaffolds show promise for the growth of ameloblast-like cells.
STEM CELLS AND DEVELOPMENT
(2021)
Article
Cell Biology
LingLing Jia, Yuta Chiba, Kan Saito, Keigo Yoshizaki, Tian Tian, Xu Han, Kanji Mizuta, Mitsuki Chiba, Xin Wang, Shahad Al Thamin, Aya Yamada, Satoshi Fukumoto
Summary: AmeloD is demonstrated to be a critical transcription factor for ameloblast differentiation, with AmeloD-KO mice showing enamel hypoplasia and downregulation of Sox21 and ameloblast differentiation marker genes. Overexpression of AmeloD can upregulate Sox21 and ameloblast differentiation marker genes, indicating its importance in amelogenesis.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Cell Biology
Kouhei Shimizu, Min Gi, Shugo Suzuki, Brian J. North, Asami Watahiki, Satoshi Fukumoto, John M. Asara, Fuminori Tokunaga, Wenyi Wei, Hiroyuki Inuzuka
Summary: Acetylation of MCL1 is a critical post-translational modification that enhances its oncogenic function, promoting apoptosis evasion of cancer cells and displaying therapeutic potential.
Article
Physiology
Aya Yamada, Keigo Yoshizaki, Masaki Ishikawa, Kan Saito, Yuta Chiba, Emiko Fukumoto, Ryoko Hino, Seira Hoshikawa, Mitsuki Chiba, Takashi Nakamura, Tsutomu Iwamoto, Satoshi Fukumoto
Summary: Connexin 43 (Cx43) is an integral membrane protein that plays a crucial role in tooth development, regulating intercellular communication through gap junction activity by modulating TGF-beta 1-mediated ERK signaling and enamel formation.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Engineering, Biomedical
Huling Lyu, Xuefeng Zhou, Yunzhu Qian, Xiaohua Liu, Gokul Gopinathan, Mirali Pandya, Chunlin Qin, Xianghong Luan, Thomas G. H. Diekwisch
Summary: The use of a PLGA-PCL scaffold coated with dopamine and collagen, enriched with PFI-2, has shown potential in enhancing bone regeneration. Applied in an experimental periodontitis model, the scaffold resulted in significant repair of periodontal tissues and the formation of highly ordered regenerated cementum.
Article
Genetics & Heredity
Gokul Gopinathan, Xinmin Zhang, Xianghong Luan, Thomas G. H. Diekwisch
Summary: In this study, the epigenetic events that limit HOX gene expression in craniofacial tissues were investigated. High levels of repressive H3K27me3 marks were found on the HOX gene promoters in undifferentiated neural crest cells, while the ratio of K4/K27 marks was less repressive in differentiated odontogenic progenitors and other craniofacial tissues. The elevated levels of HOX genes, especially those from the HOXA and HOXB clusters, were found in alveolar bone cells. The induction of HOX expression in craniofacial neural crest cells resulted in decreased mineralization markers and promoting HOX gene expression in developing teeth led to increased patterning events and gene expression.
Article
Genetics & Heredity
Gokul Gopinathan, Xianghong Luan, Thomas G. H. Diekwisch
Summary: The nonmineralized state of the mammalian periodontal ligament is controlled by an epigenetic regulation of the RUNX2 and OSX key mineralization gene promoters.
Review
Developmental Biology
Gokul Gopinathan, Thomas G. H. Diekwisch
Summary: The epigenome controls all aspects of eukaryotic development by influencing the packaging of DNA and gene expression levels. This review focuses on three major epigenetic modifications, DNA methylation, histone methylation/acetylation, and chromatin remodeling, and provides examples of their effects on early development.
JOURNAL OF DEVELOPMENTAL BIOLOGY
(2022)
Article
Developmental Biology
Mirali Pandya, Gokul Gopinathan, Connie Tillberg, Jun Wang, Xianghong Luan, Thomas G. H. Diekwisch
Summary: YAP and TAZ signaling plays a crucial role in the mineralization and cell proliferation of periodontal tissues. Loss of YAP/TAZ leads to decreased bone density, downregulation of mineralization-associated genes, increased resorption markers, and increased osteoclast numbers.
JOURNAL OF DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Xiao Sun, Hiroki Kato, Hiroshi Sato, Michiko Torio, Xu Han, Yu Zhang, Yuta Hirofuji, Takahiro A. Kato, Yasunari Sakai, Shouichi Ohga, Satoshi Fukumoto, Keiji Masuda
Summary: Research suggests that mutations in the TRPV4 gene can affect brain development, and mitochondria may be a potential therapeutic target to alleviate the symptoms associated with TRPV4-related diseases.
BIOCHEMISTRY AND BIOPHYSICS REPORTS
(2021)