Redox cycling-amplified enzymatic Ag deposition and its application in the highly sensitive detection of creatine kinase-MB

This communication reports a novel enzymatic Ag-deposition scheme combined with chemical-chemical redox cycling by reduced beta-nicotin-amide adenine dinucleotide. This novel scheme allows a higher Ag-deposition rate than a scheme using only enzymatic Ag deposition. Therefore, it can be applied for the highly sensitive detection of a cardiac biomarker, creatine kinase-MB.