Article
Chemistry, Multidisciplinary
Chong Zuo, Ruichao Ding, Xiangwei Wu, Yuanxia Wang, Guo-Chao Chu, Lu-Jun Liang, Huasong Ai, Ze-Bin Tong, Junxiong Mao, Qingyun Zheng, Tian Wang, Zichen Li, Lei Liu, Demeng Sun
Summary: This study reports a new method for protein labeling and semi-synthesis using SrtA-mediated ligation. The method shows broad tolerance for LPxT motifs and enables efficient and irreversible connection of protein substrates.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Organic
Wei- Liao, Sih-Yu Lin, Yu-Shan Kuo, Chien-Fu Liang
Summary: Sodium thiosulfate is used as the sulfur source to react with anhydrides and then with phenols, enabling site-selective acylation of phenolic hydroxyl group in the presence of other alcoholic groups. This acylation method offers operational simplicity, high efficiency, and the use of odorless reagents with low toxicity.
Article
Chemistry, Multidisciplinary
Zhengning Wang, Xuewei Wang, Penghui Wang, Junfeng Zhao
Summary: Allenone has been identified as a highly effective peptide coupling reagent for the first time, showcasing its robustness in forming peptide bonds. It is not only effective for synthesizing simple amides and dipeptides, but also suitable for peptide fragment condensation and solid-phase peptide synthesis (SPPS). This method represents a disruptive innovation in peptide synthesis by combining the advantages of conventional active esters and coupling reagents while overcoming their disadvantages.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Biochemical Research Methods
Anastasiia Antonenko, Avinash Kumar Singh, Karolina Mosna, Artur Krezel
Summary: Among various approaches for semisynthesis of natural or chemically modified products, enzyme-assisted ligation is promising and rapidly developing. In this study, a C247A mutant of Oldenlandia affinis plant ligase OaAEP1 and solid-phase peptide synthesis were used to chemoenzymatically synthesize the complete sequence of cyanobacterial metallothionein SmtA. The newly synthesized SmtA showed similar metal binding properties to the protein expressed in E. coli. This approach represents the first example of using the OaAEP1 mutant for total protein synthesis of metallothionein and has potential for synthesizing SmtA and other proteins or derivatives efficiently.
BIOCONJUGATE CHEMISTRY
(2023)
Article
Chemistry, Organic
Dipankar Chaudhuri, Rajasekaran Ganesan, Alicia Vogelaar, Mansour A. Dughbaj, Paul M. Beringer, Julio A. Camarero
Summary: A new epimerization-free approach for the Fmoc-based synthesis of murepavadin using intramolecular native chemical ligation and desulfurization reaction has been developed, resulting in a high yield of bioactive murepavadin in two steps. The synthetic peptide antibiotic showed potent activity against different clinical isolates of P. aeruginosa. This approach can be easily adapted for the production of murepavadin analogues and other backbone-cyclized peptides.
JOURNAL OF ORGANIC CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Jan Bierlmeier, Miguel Alvaro-Benito, Maren Scheffler, Kristina Sturm, Luisa Rehkopf, Christian Freund, Dirk Schwarzer
Summary: Sortase-mediated ligation is a powerful tool in protein chemistry for connecting peptides. By using switchable sortase substrates, multi-fragment SML can be achieved, expanding its applications in protein research.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Physical
Qiang Chao, Tianlu Li, Ji-Xiang Jia, Zijie Li, Peng Peng, Xiao-Dong Gao, Ning Wang
Summary: This study presents a convenient and efficient strategy for modifying glycans to obtain structurally defined oligosaccharides, which is crucial for investigating their function and biological roles. The strategy involves the use of immobilized enzymes to selectively modify glycans with terminal galactose or Sia-Gal residues, allowing the assembly of diverse sialyl-galactosylated glycans. The utility of this strategy is demonstrated by the construction of a panel of Core 2 O-GalNAc glycans.
Article
Biochemistry & Molecular Biology
Vlad-Stefan Raducanu, Daniela-Violeta Raducanu, Yujing Ouyang, Muhammad Tehseen, Masateru Takahashi, Samir M. Hamdan
Summary: TSGIT is a fusion-tag system composed of both N- and C-terminal composite fusion tags, which streamline the cloning, expression, and purification procedures to efficiently purify full-length proteins and prevent truncated forms.
Article
Chemistry, Multidisciplinary
Peisi Liao, Hongmei Liu, Chunmao He
Summary: In this study, we developed a reliable method for the synthesis of human SelF and described the synthetic route in detail. The synthesis involved a desulfurization process and removal of protective groups, resulting in the production of a large quantity of homogenous SelF. The reduction potential and oxidoreductase activity of SelF were determined, supporting its proposed function.
Article
Biochemistry & Molecular Biology
Maciej P. Paprocki, Jakob E. Rasmussen, Kasper K. Sorensen, Knud J. Jensen
Summary: The study introduces a new approach for the synthesis of peptide thioesters and hydrazides, utilizing the activation of the pGlu linker and a novel safety-catch linker. This method involves efficient amide-to-imide activation followed by nucleophilic displacement.
JOURNAL OF PEPTIDE SCIENCE
(2021)
Article
Agriculture, Multidisciplinary
Jinsong Zhao, Renshui Fan, Feng Jia, Yanbo Huang, Zhiqiang Huang, Yi Hou, Song-Qing Hu
Summary: This study achieved high-efficiency expression of butelase-1 and explored its application in food-derived ACE inhibitory peptides, proposing an efficient method for preparation and providing a promising model for its application in the food field.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Sebastian Trunschke, Emanuele Piemontese, Olaf Fuchs, Skander Abboud, Oliver Seitz
Summary: By introducing new ligation auxiliaries, this study expands the applicability of native chemical ligation and investigates the role of the pyridine-ring nitrogen in the auxiliaries. The study successfully synthesizes peptides with special structures, demonstrating that the new auxiliary has increased stability and wide application potential.
CHEMISTRY-A EUROPEAN JOURNAL
(2022)
Article
Chemistry, Multidisciplinary
Suresh S. Pujari, Mingxuan Wu, Jenna Thomforde, Zhipeng A. Wang, Christopher Chao, Noelle M. Olson, Luke Erber, William C. K. Pomerantz, Philip Cole, Natalia Y. Tretyakova
Summary: DNA-protein cross-links between DNA epigenetic mark 5-formylC and histone proteins spontaneously form in human cells, influencing chromatin structure and DNA processes. Researchers constructed site-specific, hydrolytically stable DPCs using oxime ligation, finding that histone H3-DNA crosslinks blocked DNA synthesis but were bypassed after proteolytic processing.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Multidisciplinary
Hsin-Kai Tseng, Yung-Yu Su, Ting-Wei Chang, Hsin-Chien Liu, Pei-Jhen Li, Pei-Yun Chiang, Chun-Cheng Lin
Summary: By using PmST1, acceptor-mediated regioselective sialylation (AMRS) was achieved on the nonreducing end GalNH(2) or GalAz, allowing efficient assembly of C5 and C8-modified sialic acid to synthesize the GAA-7 glycan moiety, one of the echinodermatous gangliosides with higher neuritogenic activity.
CHEMICAL COMMUNICATIONS
(2021)
Review
Chemistry, Medicinal
Masaya Denda, Akira Otaka
Summary: This article describes a method for converting natural peptides to thioesters, which promotes the preparation of synthetic modified artificial proteins.
CHEMICAL & PHARMACEUTICAL BULLETIN
(2022)
Article
Chemistry, Applied
Jiayi Huang, Ka H. Wong, Wei Liang Tan, Stephanie V. Tay, Shujing Wang, James P. Tam
Summary: The discovery of a novel hyperstable beta-lybatide from wolfberry plant as a carboxypeptidase inhibitor is reported. This peptide, belonging to a different CRP family, displayed a knottin-like structure and showed potent inhibitory activity against carboxypeptidase, contributing to wolfberry biological activities. Bioinformatics analysis revealed additional similar plant carboxypeptidase inhibitors, indicating beta-lybatide as the first and smallest plant-derived hyperstable carboxypeptidase inhibitor from functional foods.
Article
Biochemical Research Methods
Dingpeng Zhang, Zhen Wang, Side Hu, Ning-Yu Chan, Heng Tai Liew, Julien Lescar, James P. Tam, Chuan-Fa Liu
Summary: This study explores the use of asparaginyl endopeptidases (AEPs) in hydrazinolysis reactions, finding that hydrazine and hydroxylamine can efficiently resolve the thioester intermediate to produce peptide and protein products with specific functional groups. The results demonstrate that AEP-mediated protein hydrazinolysis is a simple and straightforward strategy for the precision manufacturing of protein bioconjugates.
BIOCONJUGATE CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Dingpeng Zhang, Zhen Wang, Side Hu, Julien Lescar, James P. Tam, Chuan-Fa Liu
Summary: In the past two decades, there has been a growing demand for new protein modification methods in the biotech industry and biomedical research communities. Peptidyl Asx-specific ligases (PALs), a newly discovered class of peptide ligases, have emerged as powerful biotechnological tools. The use of a new PAL, VyPAL2, was successfully demonstrated for a diverse range of protein modifications, including protein labelling, inter-protein ligation, and protein cyclization. These PALs have the potential to revolutionize site-specific protein modification in basic research and drug discovery.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Side Hu, Abbas El Sahili, Srujana Kishore, Yee Hwa Wong, Xinya Hemu, Boon Chong Goh, Sang Zhipei, Zhen Wang, James P. Tam, Chuan-Fa Liu, Julien Lescar
Summary: Peptide ligases are versatile enzymes that are capable of precise protein conjugation for bioengineering applications. In this study, crystal structures of VyPAL2, a highly catalytically active peptide asparaginyl ligase (PAL) from Viola yedoensis, were reported, revealing how PAL specifically recognizes its substrate and providing important insights into enzyme activity.
Article
Microbiology
Jun Jie Wong, Foo Kiong Ho, Pei Yi Choo, Kelvin K. L. Chong, Chee Meng Benjamin Ho, Ramesh Neelakandan, Damien Keogh, Timothy Barkham, John Chen, Chuan Fa Liu, Kimberly A. Kline
Summary: This study reveals the polymicrobial interaction between Escherichia coli and Staphylococcus aureus in wound infections. It shows that E. coli kills S. aureus through colibactin and this interaction is regulated by the BarA-UvrY two component system.
Article
Chemistry, Multidisciplinary
Yiyin Xia, Fupeng Li, Xiaohong Zhang, Seetharamsing Balamkundu, Fan Tang, Side Hu, Julien Lescar, James P. Tam, Chuan-Fa Liu
Summary: Enzymatic peptide ligation, particularly through peptidyl asparaginyl ligases (PALs), is a promising tool in studying protein functions and developing protein therapeutics. However, the reversibility of PAL-mediated ligation limits its efficacy, requiring a large excess of one of the partners. A method utilizing glutaminyl cyclase (QC)-catalyzed pyroglutamyl formation is presented to make intermolecular ligation irreversible and improve reaction yields.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Plant Sciences
Xinya Hemu, Ning-Yu Chan, Heng Tai Liew, Side Hu, Xiaohong Zhang, Aida Serra, Julien Lescar, Chuan-Fa Liu, James P. P. Tam
Summary: In this study, 16 new asparaginyl endopeptidases (AEPs) were identified from 875 plant species, and two conserved glycine residues were found to be critical for differentiating ligases and proteases. These findings provide important clues for further identification and study of these two families.
Article
Biochemistry & Molecular Biology
Niying Chua, Yee Hwa Wong, Abbas El Sahili, Chuan Fa Liu, Julien Lescar
Summary: We engineered a highly active asparaginyl ligase that does not require acid activation and can be stored for up to 2 years. This ligase can be used for peptide cyclization and protein conjugation applications.
Article
Cell Biology
Bamaprasad Dutta, Shining Loo, Antony Kam, Siu Kwan Sze, James P. Tam
Summary: Hypoxia-induced vascular endothelial dysfunction (VED) is a significant contributor to severe human diseases. The discovery of ginsentide TP1, a microprotein in ginseng, has shown promising results in reducing vascular dysfunction. This study identifies new proteins synthesized in hypoxia and demonstrates the protective effects of ginsentide TP1 against hypoxia and ER stress in human endothelial cells.
Article
Biochemistry & Molecular Biology
James P. Tam, Jiayi Huang, Shining Loo, Yimeng Li, Antony Kam
Summary: Coffee processing waste contains novel microproteins with cell-penetrating and metal ion-binding abilities, including a ginsentide-like microprotein that can target intracellular proteins. These findings suggest that coffee by-products could be a potential source of bioactive peptides for targeting intracellular proteins.
Review
Chemistry, Multidisciplinary
Dan He, Yawen Li, Fuwen Zhao, Yuze Lin
Summary: High trap density in organic solar cells leads to localized charge carriers and reduced carrier lifetime, limiting device efficiency. This feature article summarizes the recent advances of trap suppression by material design and device engineering.
CHEMICAL COMMUNICATIONS
(2024)
Review
Chemistry, Multidisciplinary
Olivier Monfort, Arshitha Madhusudhan, Martin Motola
Summary: This article presents the advantages of coupling photocatalysis and Fenton-based processes for environmental remediation and discusses their synergy. It critically examines the simultaneous triggering of these two processes and provides insights into research approaches. The study aims to enhance understanding of this complex process and highlight the potential integration of new catalysts in current wastewater treatment technology.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Manideepa Dhar, Chittaranjan Mishra, Avijit Das, Uttam Manna
Summary: An approach of 'polymerization of monomers in its aggregated form' has been introduced to tailor the water wettability of fibrous and porous substrates, achieving hydrophobicity to superhydrophobicity transition, as well as patterned wettability. This facile chemical method provides a durable coating with adjustable and patterned wettability for various potential applications.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Saswat Ranjan Bhoi, Chhanda Debnath, Shikha Gandhi
Summary: In this article, a novel catalytic reaction using Pd(0) and Bronsted acid is reported for the synthesis of biologically important imidazolidinone-fused N-heterocycles through redox-neutral intramolecular N-allylation of indoles and pyrroles with alkynes. This atom-economical method is applicable to a wide range of substrates and eliminates the need for leaving groups or oxidizing agents commonly used in traditional allylation reactions.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Longkun Chen, Mingshuai Zhang, Meichen Liu, Zhuoyuan Liu, Yuetong Qiu, Zhilai Zhang, Fuchao Yu, Jiuzhong Huang
Summary: In this study, an efficient Rh(III)-catalyzed selective mono- and dual-C-H bond functionalization/cyclization with iodonium ylide as the sole coupling partner was demonstrated. Fused benzodiazepine skeletons were obtained in excellent yields, providing an improved approach to dual C-H unsymmetrical functionalization.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Alice Kennett, Sven Epple, Gabriella van der Valk, Irene Georgiou, Evelyne Gout, Romain R. Vives, Angela J. Russell
Summary: This study presents the design and synthesis of sulfated disaccharide inhibitors based on IdoA(2S)-GlcNS(6S), which showed potent inhibition of Sulf-2. The results suggest that IdoA(2S)-GlcNS(6S) is the shortest fragment size required for effective inhibition of Sulfs, and a trisulfated disaccharide is identified as the minimal fragment size of heparan sulfate for effective endosulfatase inhibition.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Mauro Garbini, Andrea Brunetti, Riccardo Pedrazzani, Magda Monari, Massimo Marcaccio, Giulio Bertuzzi, Marco Bandini
Summary: The self-adaptability of galvanostatic electrolysis was found to be helpful in a multistage chemo- and diastereoselective electrochemically promoted cyclodimerization of chalcones. Through a series of reductive events, densely functionalized cyclopentanes with five contiguous stereocenters were obtained (25 examples, yields up to 95%, dr values up to >20:1). Further experimental and electrochemical investigations indicated the crucial role of dynamic kinetic resolution of the aldol intermediate in the reaction mechanism.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Dhananjay S. Nipate, Neha Meena, Prakash N. Swami, Krishnan Rangan, Anil Kumar
Summary: In this study, the synthesis of functionalized benzo[a]phenazines and indazolo[2,3-a]quinolines was achieved through Rh(III)-catalyzed oxidative annulation of 2-arylquinoxalines and 2-aryl-2H-indazoles with allyl alcohols. The method showed a wide substrate scope, excellent functional group tolerance, good to high yields of annulated products, and the capability for scaled-up synthesis. A tentative mechanism of the annulation reaction was proposed based on a preliminary mechanistic investigation.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Ruonan Zhang, Abudukadi Tudi, Xia Yang, Xuping Wang, Zhihua Yang, Shujuan Han, Shilie Pan
Summary: A new lead tellurium borate material with a unique fundamental building block [B4O10] was synthesized and found to exhibit high birefringence. The structure-property relationship was discussed using first-principles calculations.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Amresh Kumar Singh, Baishali Thakurta, Anupam Giri, Monalisa Pal
Summary: This study introduces three key concepts for the quick synthesis of large-scale high-quality 2D materials films and their utilization in device fabrication.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Reina Hagiwara, Shun Nishimura, Kosuke Okeyoshi
Summary: A copolymer-conjugated nanocatalytic system has been developed to improve electron transfer and enhance photoinduced H2 generation.
CHEMICAL COMMUNICATIONS
(2024)
