期刊
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
卷 63, 期 -, 页码 644-649出版社
ELSEVIER
DOI: 10.1016/j.msec.2016.03.046
关键词
Sacrificial microsphere; Cell encapsulation; Tumor spheroid formation; 3D drug testing model
资金
- Ministry of Education, Singapore [AcRF Tier 2 ARC 1/13, AcRF Tier 1 RG 30/15]
Sacrificial gelatin microspheres can be developed as a cell delivery vehicle for non-anchorage dependent cells its incorporation into a macroscopic scaffold system not only allows the cells to be cultured in suspension within cavities left behind by the sacrificial material, it also allows scaffold-free tissue development to be confined within the cavities. In this study, dense and highly viable hepatocarcinoma spheroids were developed by means of encapsulation in sacrificial gelatin microspheres produced via a simple water-in-oil emulsion technique. By initial selection of microsphere size and distribution, spheroid size can be controlled for various applications such as uniform tumor spheroids as a reproducible three-dimensional drug screening and testing platform that better mimics the in vivo nature of tumors (instead of conventional monolayer culture), as this study has suggested as a proof-of-concept with chemotherapy drug Doxorubicin. (C) 2016 Elsevier B.V. All rights reserved.
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