Article
Chemistry, Applied
Junfu Ji, Xinyu Liu, Xiaosong Hu, Fang Chen, Christoph Bueschl, Rainer Schuhmacher, Andrew L. Waterhouse, Lingjun Ma
Summary: This study successfully identified quinone reaction products in wine using a stable-isotope labelling approach combined with high-resolution mass spectrometry. Different nucleophiles were found to react in white wines and red wines.
Article
Pharmacology & Pharmacy
Sabrina Wohlfart, Michael Kilian, Philip Storck, Thomas Gutsmann, Klaus Brandenburg, Walter Mier
Summary: A novel method for precise quantification of Pep19-2.5 in biological samples has been developed in this study, which is crucial for the treatment of sepsis. The method utilizes mass spectrometry and labeled amino acid residues to reliably quantify the peptide content.
Article
Chemistry, Applied
Wei Xia, Xiangjun Fang, Yuan Gao, Weijie Wu, Yanchao Han, Ruiling Liu, Hailong Yang, Hangjun Chen, Haiyan Gao
Summary: Food quality, safety, and nutrient metabolism play a key role in human health. However, contaminants and metabolic dysregulation in food can be problematic. Chromatography-mass spectrometry technology, especially when combined with stable isotopes, is widely used in food analysis and nutrient metabolism research. This study provides an overview of stable isotope technology applications in quantifying contaminant residues and nutrient metabolism, aiming to contribute to effective analysis techniques for protecting food quality and human health.
Review
Endocrinology & Metabolism
Manuel Grima-Reyes, Adriana Martinez-Turtos, Ifat Abramovich, Eyal Gottlieb, Johanna Chiche, Jean-Ehrland Ricci
Summary: This article highlights the importance and methods of studying tumor metabolism using in vivo isotope tracing. Research findings suggest that metabolic dependencies in cancers are influenced by tissue environment, cancer lineage, and genetic events, providing crucial clues for identifying key alterations contributing to cancer onset and progression.
MOLECULAR METABOLISM
(2021)
Review
Chemistry, Analytical
Di Yu, Lina Zhou, Xinyu Liu, Guowang Xu
Summary: The introduction of stable isotope labeling (SIL) technology has become a routine tool for functional metabolomics studies and allows for a deeper understanding of dynamic metabolic regulation in organisms. Mass spectrometry (MS) techniques have shown great vitality in this field by utilizing the different mass spectrometric characteristics between endogenous metabolites and their isotope-labeling forms. Recent advances in MS-based stable isotope-resolved metabolomics (SIRM) methods have expanded the breadth and depth of SIRM research by improving sample pretreatment, MS analysis, and data analysis. This review describes the recent progress of SIRM methods, summarizes their applications in metabolite identification, accurate quantification, and metabolic flux analysis, and discusses current limitations and challenges.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Applied
Philippe Tixier, Denis Loeillet, Mathieu Coulis, Thierry Lescot, Luc de Lapeyre de Bellaire
Summary: With the increasing production of organic bananas, it is crucial to monitor compliance with organic specifications. This study found that the delta N-15 values of organic bananas were significantly higher than conventional ones at farms around the world, while the type of fertilization did not significantly affect delta C-13 values.
Review
Cardiac & Cardiovascular Systems
Anja Karlstaedt
Summary: Metabolic remodeling plays a critical role in cardiovascular diseases, and stable-isotope tracer measurements are a powerful tool for evaluating cardiac metabolism changes. This review summarizes techniques, mathematical concepts, analytical methods, and the use of metabolic models in cardiovascular research.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2021)
Article
Environmental Sciences
Daniel F. Araujo, Joel Knoery, Nicolas Briant, Nathalie Vigier, Emmanuel Ponzevera
Summary: The advent of Multicollector ICP-MS has opened the door to studying nontraditional isotopes in marine metal contamination, providing valuable tools for understanding geochemical and ecotoxicological aspects. However, research on these applications is still in its early stages, with limited information on recent developments and obstacles. This paper aims to fill this gap and encourage the marine scientific community to utilize this newly available information for chemical risk assessment, biomonitoring, and examining the trophic transfer of metal contaminants.
MARINE POLLUTION BULLETIN
(2022)
Review
Nutrition & Dietetics
Sulagna Bandyopadhyay, Sindhu Kashyap, Juliane Calvez, Sarita Devi, Dalila Azzout-Marniche, Daniel Tome, Anura Kurpad, Claire Gaudichon
Summary: Recent expert consultations have stressed the importance of the Digestible Indispensable Amino Acid Score (DIAAS) as a measure of protein quality, requiring human measurements of amino acid digestibility at the end of the small intestine. Efforts have been made to develop noninvasive or minimally invasive methods to measure amino acid digestibility in humans, but these methods still need validation.
ADVANCES IN NUTRITION
(2022)
Review
Chemistry, Multidisciplinary
Alexandre Labiche, Augustin Malandain, Maxime Molins, Frederic Taran, Davide Audisio
Summary: In contrast to stable and natural abundant carbon-12, the synthesis of organic molecules with carbon (radio) isotopes requires careful planning and optimization due to the challenges of radiochemical requirements. Recently, carbon isotope exchange technologies have emerged as effective methods for late-stage labeling, utilizing primary radiolabeled building blocks and various activation processes. This review provides a brief overview of these technologies and their potential impact on radiosynthesis.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Plant Sciences
Silvia De Rose, Yukari Kuga, Fabiano Sillo, Valeria Fochi, Naoya Sakamoto, Jacopo Calevo, Silvia Perotto, Raffaella Balestrini
Summary: Orchid mycorrhiza (OM) is a unique symbiosis between plants and fungi, where the mycorrhizal fungus provides carbon, phosphorus, nitrogen, and possibly sulfur to the host orchid. This study investigated sulfur metabolism and transfer in the OM symbiosis using advanced imaging techniques and gene expression analysis. The findings revealed that the fungi actively contribute to sulfur supply, and the transfer most likely occurs in organic forms. This study adds new insights into the nutritional framework of OM symbiosis.
Article
Biochemical Research Methods
Allyson L. Mellinger, Kenneth P. Garrard, Sitora Khodjaniyazova, Zahid N. Rabbani, Michael P. Gamcsik, David C. Muddiman
Summary: This study presents a method using multiple infusion start time protocol, stable isotope labeling technology, and high-resolution accurate mass-mass spectrometry imaging to study spatial changes in glutathione kinetics in sectioned mouse liver tissues. The results provide insights into the heterogeneity of glutathione flux in diseased and healthy tissues.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Marine & Freshwater Biology
Eric Bollinger, Jochen P. Zubrod, Marco Konschak, Ralf Schulz, Mirco Bundschuh
Summary: Stable isotope analysis is an important technique in food web ecology, but its effectiveness can be unclear in complex systems. The use of heavy isotope tracers has been suggested as a way to enhance the utility of stable isotope analysis, but this study found that labeling could potentially affect isotopic fractionation and bias conclusions. While the physiological and activity levels of organisms in autotrophy-based and detritus-based aquatic food webs were not significantly affected by labeling, the study supported the isotopic redundancy hypothesis, which suggests that metabolic processes can be altered by discrete quantum mechanical states.
AQUATIC TOXICOLOGY
(2023)
Article
Soil Science
Zhiying Liang, Binbin Cao, Yapeng Jiao, Conghui Liu, Xiushuang Li, Xiangtian Meng, Jianglan Shi, Xiaohong Tian
Summary: Nitrogen fertilization can impact soil respiration by affecting the decomposition of soil organic matter and plant residues through microbial biomass. The conversion of mineral nitrogen to stable organic nitrogen (humus nitrogen) remains unclear, particularly in the presence of straw. Experiment results reveal a two-phase effect of nitrogen addition on CO2 emissions, indicating rapid utilization by microorganisms. Additionally, the study shows that stable organic nitrogen in humus can be converted by microbes, and the combination of nitrogen and straw promotes this conversion.
APPLIED SOIL ECOLOGY
(2022)
Article
Biochemical Research Methods
Juho Heininen, Ulrika Julku, Timo Myohanen, Tapio Kotiaho, Risto Kostiainen
Summary: A new multiplexed LC-MS/MS method was developed for analyzing amino acids in mouse brain microdialysis samples, providing good chromatographic performance and high sensitivity. The method showed good repeatability and linearity, indicating its quantitative performance. The method was sensitive enough to detect low concentrations of amino acids in brain microdialysis samples.
JOURNAL OF CHROMATOGRAPHY A
(2021)