期刊
MARINE GENOMICS
卷 28, 期 -, 页码 37-39出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.margen.2016.04.009
关键词
Transcriptome; RNA-seq; Antarctic; Copepod; Tigriopus kingsejongensis; Lipid metabolism
资金
- Marine Biotechnology Program - Ministry of Oceans and Fisheries of Korea [PJT200620]
- Korea-Polar Ocean Development: K-POD - Ministry of Oceans and Fisheries, Korea [PM15050]
The whole transcriptome of the Antarctic copepod (Tigriopus kingsejongensis) was sequenced using Illumina RNA-seq. De novo assembly was performed with 64,785,098 raw reads using Trinity, which assembled into 81,653 contigs. TransDecoder found 38,250 candidate coding contigs which showed homology to other species by BLAST analysis. Functional gene annotation was performed by Gene Ontology (GO), InterProScan, and KEGG pathway analyses. Finally, we identified a number of expressed gene catalog for T. kingsejongensis that is a useful model animal for gene information-based polar research to uncover molecular mechanisms of environmental adaptation on harsh environments. In particular, we observed highly developing lipid metabolism in T. kingsejongensis directly compared to those of the Far East Pacific coast copepod Tigriopus japonicus at the transcriptome level. (C) 2016 Elsevier B.V. All rights reserved.
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