Article
Chemistry, Medicinal
Xiao-Meng Sun, Zhao Xue, Mei-Ling Sun, Yi Zhang, Yu-Zhong Zhang, Hui-Hui Fu, Yu-Qiang Zhang, Peng Wang
Summary: In this study, a novel alginate lyase, AlyC8, with two functional catalytic domains (CD1 and CD2) was discovered. Both CD1 and CD2 showed synergistic effects in alginate degradation. The results demonstrated that CD1 plays a major role in the degradation of alginate by AlyC8, and CD1 and CD2 have different substrate specificities.
Article
Chemistry, Medicinal
Li Sha, Minghai Huang, Xiaonan Huang, Yongtong Huang, Ensi Shao, Xiong Guan, Zhipeng Huang
Summary: A highly active novel alginate lyase was obtained from marine bacteria. The enzyme showed preference for polyM as substrate, and its activity was enhanced by K+ and inhibited by Fe2+ and Cu2+. It has promising industrial application prospects.
Article
Biochemistry & Molecular Biology
Zhongbin Xiao, Kuikui Li, Tang Li, Fanxing Zhang, Jiayi Xue, Miao Zhao, Heng Yin
Summary: Alginate lyase VwAlg7A, a novel PL7 family exolytic alginate lyase, was successfully heterologously expressed in E. coli BL21 (DE3). VwAlg7A showed specificity towards poly-guluronate and exhibited optimal temperature and pH at 30 degrees C and 7.0, respectively. The activity of VwAlg7A could be inhibited by Ni2+, Zn2+, and NaCl, and its kinetic parameters were determined as K-m of 36.9 mg/ml and V-max of 395.6 mu M/min. ESI and HPAEC-PAD results confirmed the exolytic mode of sugar bond cleavage by VwAlg7A, and important catalytic residues were identified through molecular docking and mutagenesis experiments.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Xinxiu Wang, Wei Xu, Quanyu Dai, Xiaoyong Liu, Cuie Guang, Wenli Zhang, Wanmeng Mu
Summary: Currently, marine sugars have gained increased attention due to their unique physiological effects. Alginate oligosaccharides (AOS), degradation products of alginate, find applications in the fields of food, cosmetic, and medicine. A novel alginate lyase from Paenibacillus ehimensis (paeh-aly) showed potential for industrial production of AOS due to its excellent thermostability and efficiency.
ENZYME AND MICROBIAL TECHNOLOGY
(2023)
Article
Chemistry, Medicinal
Rui Yin, Yan-Jun Yi, Zhuo Chen, Bao-Xun Wang, Xue-Han Li, Yan-Xia Zhou
Summary: In this study, a new exo-alginate lyase named Alys1 with high activity was cloned from a marine strain, showing cold-adapted characteristics and efficient degradation of alginates at low temperatures. Alys1 can depolymerize alginates polyM and polyG, producing monosaccharides as minimal alginate oligosaccharides, and demonstrates bifunctionality with a preference for M over G. These properties make Alys1 a valuable candidate for both basic research and industrial applications.
Article
Biotechnology & Applied Microbiology
Xiao-Han Wang, Xiao-Hui Sun, Xiu-Lan Chen, Ping-Yi Li, Qi-Long Qin, Yu-Qiang Zhang, Fei Xu
Summary: This study reports a novel alginate lyase, AlyC6', with two functional alginate lyase domains that synergize intramolecularly. Furthermore, it investigates the distribution of alginate lyases with multiple domains based on bioinformatics analysis. This study provides new insights into bacterial alginate lyases and alginate degradation.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Fengchao Zhang, Zheng Fu, Luyao Tang, Zhelun Zhang, Feng Han, Wengong Yu
Summary: The novel exo-type alginate lyase VsAly7D isolated from brown algae showed the highest activity at 35 degrees C, with considerable activity at 20 and 30 degrees C, while exhibiting optimal activity under alkaline conditions. VsAly7D demonstrated efficient degradation of alginate, polyM, and polyG, making it a promising tool enzyme for bioethanol production from brown algae.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Shengsheng Cao, Li Li, Qian Li, Ling Jiang, Benwei Zhu, Zhong Yao
Summary: A novel alginate lyase, AlyPL17, was cloned and characterized from Pedobacter hainanensis NJ-02. It exhibited efficient catalytic activity towards polymannuronic acid, polyguluronic acid, and alginate sodium. AlyPL17 showed maximum activity at 45°C and pH 9.0, and degraded alginate through the cooperative action of two structural domains in an exolytic mode.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Chemistry, Medicinal
Mingpeng Wang, Lei Chen, Zhengyu Lou, Xueting Yuan, Guiping Pan, Xiaoyan Ren, Pengyu Wang
Summary: Alginate oligosaccharides (AOS) are low molecular weight alginate with improved water solubility and comprehensive health benefits. In this study, a novel alginate lyase called Algpt was discovered and characterized from a marine bacterium. Algpt exhibited high stability and adaptability, making it suitable for efficient preparation of AOS with different sizes and AOS-based products.
Article
Biochemistry & Molecular Biology
Licheng Zhou, Qing Meng, Ran Zhang, Bo Jiang, Qun Wu, Jingjing Chen, Tao Zhang
Summary: In this study, a comprehensive strategy was proposed to improve the thermostability of alginate lyase with strict substrate specificity. Through sequence-based and structure-based analysis, as well as computer-aided Delta Delta Gfold value calculation, four point mutations were selected and combined to generate a mutant with increased thermostability and retained enzyme activity.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Food Science & Technology
Qing Meng, Licheng Zhou, Hinawi A. M. Hassanin, Bo Jiang, Yongchuang Liu, Jingjing Chen, Tao Zhang
Summary: Alginate lyase Aly01, containing CBM32 and catalytic domain, was identified from Vibrio natriegens. Truncation of CBM32 resulted in decreased enzyme activity and thermostability decline, affecting the release of trisaccharide products. Docking study confirmed interactions between CBM32 and catalytic domain during substrate degradation.
Article
Biochemistry & Molecular Biology
Qing Guo, Meiling Dan, Yuting Zheng, Ji Shen, Guohua Zhao, Damao Wang
Summary: In this study, a novel PL-6 alginate lyase was characterized, and a comprehensive strategy for designing heat-resistant mutants was proposed. The thermostability of AlyRm6A was improved by introducing mutations, resulting in increased half-lives and melting point temperatures. Molecular dynamics simulation analysis revealed the mechanisms underlying the enhanced stability of AlyRm6A mutants. These findings suggest that AlyRm6A mutants have the highest thermostability among PL-6 alginate lyases and could be used for industrial production of alginate oligosaccharides.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biotechnology & Applied Microbiology
Xiu Zhang, Wei Li, Lixia Pan, Liyan Yang, Hongliang Li, Feng Ji, Yunkai Zhang, Hongzhen Tang, Dengfeng Yang
Summary: This study reports the enhancement of thermostability of FlAlyA, an alginate lyase with industrial potential. By computer-aided calculation and site-directed mutagenesis, two single-point mutants with better thermostability were optimized and the mechanisms of these mutants were investigated. The expression level of the mutants was greatly increased, making them potential candidates for the industrial production of alginate oligosaccharides.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Medicinal
Zhiqiang Shu, Gongming Wang, Fang Liu, Yingjiang Xu, Jianan Sun, Yang Hu, Hao Dong, Jian Zhang
Summary: Alginate lyase Alg4755, identified, heterologously expressed, and characterized in this study, showed high product specificity and desirable catalytic properties. It exhibited optimal activity at 35°C and pH 8.0, and demonstrated high thermal and pH stability. The hydrolysis products catalyzed by Alg4755 were mainly alginate disaccharides with a small amount of alginate trisaccharides.
Article
Agriculture, Multidisciplinary
Fen Yan, Junying Chen, Ting Cai, Jinfu Zhong, Shaolong Zhang
Summary: A novel bifunctional alginate lyase, BY17PV7, was cloned and expressed in Escherichia coli BL21(DE3). BY17PV7 showed the greatest activity at 43 degrees C and pH 8.9, with a higher affinity for polyG. It exhibited a wide range of substrate specificity and good degradation effects. Furthermore, the released AOS by BY17PV7 showed strong antioxidant ability.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2022)