Article
Biochemistry & Molecular Biology
Jerome Jeyakumar John Martin, Qiufei Wu, Meili Feng, Rui Li, Lixia Zhou, Shuyan Zhang, Cheng Yang, Hongxing Cao
Summary: This study utilized metabolite and mass spectral analysis to investigate the physiological processes that regulate oil synthesis during oil palm fruit ripening. The results showed significant changes in lipid metabolites at different developmental stages of the fruit, which were associated with specific protein accumulations. This study provides important insights into the role of lipid metabolism in oil biosynthesis mechanisms.
Article
Chemistry, Medicinal
Tiago A. Conde, Bruna F. Neves, Daniela Couto, Tania Melo, Bruno Neves, Margarida Costa, Joana Silva, Pedro Domingues, M. Rosario Domingues
Summary: The study used gas-chromatography mass-spectrometry to characterize the fatty acid profiles of seven different commercial microalgae, revealing unique compositions among them, especially in terms of omega-3 fatty acids, with species of the same phylum showing similar trends. Additionally, the research found that different lipid extracts exhibited comparable antioxidant activities, further demonstrating the potential of microalgae as sustainable and healthy sources of essential lipids.
Article
Biology
Francisca Marques, Diana Lopes, Tiago Conde, Tania Melo, Joana Silva, Maria Helena Abreu, Pedro Domingues, Maria Rosario Domingues
Summary: Macro- and microalgae are rich sources of lipids with nutritional quality and bioactivities for human health. This study characterized the lipidome of a blend of macro- and microalgae and evaluated its antioxidant capacity. The results show promising potential for food, nutraceutical, and biotechnological applications.
Article
Biochemical Research Methods
Adriana Zardini Buzatto, Abeer Malkawi, Essa M. Sabi, Ahmed H. Mujamammi, Liang Li, Anas M. Abdel Rahman
Summary: Dexamethasone, a synthetic glucocorticoid used to treat abnormal immune responses and inflammation, can have severe adverse effects with prolonged high-dose usage. Comprehensive lipidomic analyses on rat tissues showed significant alterations in phosphatidic acids, glycerophospholipids, plasmalogens, and fatty acids following long-term dexamethasone treatment.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Dermatology
Ellie Choi, Sartaj Ahmad Mir, Shanshan Ji, Xue Ting Ooi, Esther W. L. Chua, Yeo Yi Wei, Markus R. Wenk, Anne K. Bendt, Nisha Suyien Chandran
Summary: In this study, the plasma lipidomic profiles of Hidradenitis suppurativa (HS) patients and control subjects were compared. Mild changes in the lipidomic profiles were found, and certain lipid species were associated with disease activity and severity.
JOURNAL OF DERMATOLOGICAL SCIENCE
(2022)
Article
Multidisciplinary Sciences
Angela Criscuolo, Palina Nepachalovich, Diego Fernando Garcia-del Rio, Mike Lange, Zhixu Ni, Massimo Baroni, Gabriele Cruciani, Laura Goracci, Matthias Blueher, Maria Fedorova
Summary: This study utilizes bioinformatics and LC-MS/MS technologies to uncover the diversity and specificity of modified lipids in human blood plasma of lean and obese individuals, revealing the significant alteration of endocrine signaling by oxidized lipids in metabolic disorders.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Andrey B. Imbs, Peter V. Velansky
Summary: The study analyzed the molecular species compositions of diacylglycerol ethers (DAGE) and structural glycerophospholipids (GPL) in the deep-sea coral Paragorgia arborea, providing important information for lipidomic studies of deep-sea corals.
SCIENTIFIC REPORTS
(2021)
Article
Biochemical Research Methods
Lauren M. Bishop, Oliver Fiehn
Summary: Large-scale lipidomic analyses have been limited by the cost and accessibility of traditional venipuncture sampling. Microsampling techniques offer a less-invasive and more accessible alternative. From a single drop of blood, plasma separation cards (PSC) deliver two volumetric dried plasma samples which are studied here for profiling endogenous blood lipids.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Food Science & Technology
Christiana Mantzourani, Charikleia S. Batsika, Maroula G. Kokotou, George Kokotos
Summary: A new method was developed to simultaneously determine multiple fatty acids in yogurt samples, including both common and uncommon ones. The concentrations of various fatty acids in the samples allowed the discrimination between cow and sheep yogurt.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Plant Sciences
Hannah J. Lusk, Nicholas Neumann, Madeline Colter, Mary R. Roth, Pamela Tamura, Libin Yao, Sunitha Shiva, Jyoti Shah, Kathrin Schrick, Timothy Durrett, Ruth Welti
Summary: A mass spectrometry-based screening identified a mutant Salk_109175C with altered leaf chloroplast glycerolipid composition. Further analysis revealed that the altered lipid composition phenotype was caused by a splice-site mutation in the At4g30950 locus, which resulted in the functional loss of the plastidial omega-6 desaturase FAD6.
PLANT AND CELL PHYSIOLOGY
(2022)
Article
Multidisciplinary Sciences
Amanda Gisler, Kapil Dev Singh, Jiafa Zeng, Martin Osswald, Mo Awchi, Fabienne Decrue, Felix Schmidt, Noriane A. Sievi, Xing Chen, Jakob Usemann, Urs Frey, Malcolm Kohler, Xue Li, Pablo Sinues
Summary: Exhaled breath analysis provides valuable molecular-level information and has promising potential for precision medicine. However, the lack of validation in multicenter trials hinders the transition of breath tests into routine clinical practice. In this study, an interoperability framework was developed and applied, revealing a technical variability of approximately 20% and identifying a core signature of the human exhaled metabolome. The results showed high inter-subject variability for certain metabolic classes but relative stability in other regions, such as the TCA cycle. This framework and the overview of metabolic coverage will pave the way for future large-scale multicenter trials.
Article
Agriculture, Multidisciplinary
Yi Wang, Jinqiu Wang, Hanmei Li, Yu Xiao, Putri Widyanti Harlina, Fang Geng
Summary: The accumulation of lipids in egg yolk during its formation was studied by researchers in both food science and animal science. Results showed significant differences in fatty acid profiles and lipidomes at different stages of egg yolk formation. Transport and accumulation of lipids in egg yolk are dynamically adjusted, with different lipid molecular species having different timing and transport mechanisms.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2023)
Article
Agricultural Engineering
Anil Kumar Patel, Ajeet Singh Chauhan, Prashant Kumar, Philippe Michaud, Vijai Kumar Gupta, Jo-Shu Chang, Chiu-Wen Chen, Cheng-Di Dong, Reeta Rani Singhania
Summary: This article provides an update on the production of essential fatty acids from potential microbes and explores novel strategies to promote the accumulation of omega-3 and omega-6 through engineering and omics approaches.
BIORESOURCE TECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Ian C. H. Lee, Sergey Tumanov, Jason W. H. Wong, Roland Stocker, Joshua W. K. Ho
Summary: Mass spectrometry-based untargeted metabolomic and lipidomic approaches are widely used in biomedical research. A sample extraction method called Multi-ABLE has been developed for concurrent generation of proteomic, metabolomic, and lipidomic data. However, there is a lack of a unified bioinformatics pipeline for processing and analysis of these data. A new R pipeline called MultiABLER is presented here as a unified and simple upstream processing and analysis pipeline for metabolomics and lipidomics datasets.
Article
Chemistry, Analytical
Laura S. Bailey, Dilip Prajapati, Kari B. Basso
Summary: A new method for lipidomic normalization, using the sulfo-phospho-vanillin assay (SPVA), has been proposed. The assay shows promising results in terms of accuracy and precision, making it a potential normalization method for lipidomics.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Felisa Rey, Paulo Cartaxana, Susana Aveiro, Michael Greenacre, Tania Melo, Pedro Domingues, M. Rosario Domingues, Sonia Cruz
Summary: A study on the sea slug Elysia timida revealed that light intensity influences the degradation of stolen chloroplasts (kleptoplasts), with older kleptoplasts being targeted for degradation. The lipidome of E. timida showed differences under different light treatments, indicating a light-driven remodelling of the lipidome.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2023)
Article
Biology
Francisca Marques, Diana Lopes, Tiago Conde, Tania Melo, Joana Silva, Maria Helena Abreu, Pedro Domingues, Maria Rosario Domingues
Summary: Macro- and microalgae are rich sources of lipids with nutritional quality and bioactivities for human health. This study characterized the lipidome of a blend of macro- and microalgae and evaluated its antioxidant capacity. The results show promising potential for food, nutraceutical, and biotechnological applications.
Article
Chemistry, Analytical
Sara S. Marques, Ines I. Ramos, Carla Silva, Luisa Barreiros, Maria R. Domingues, Marcela A. . Segundo
Summary: The concentration of nanoparticles (NPs) is crucial for the development and quality control of nanocarriers. This study proposes a miniaturized automated method to measure NPs concentration, which is fast, simple, and suitable for research and quality control operations. The method utilizes the measurement of light scattering caused by NPs passing through an optical path, achieving accurate and efficient quantification of NPs concentration.
ANALYTICAL CHEMISTRY
(2023)
Review
Food Science & Technology
Tiago Jardim Costa, M. Rosario M. Domingues, Eliana Alves
Summary: Tree nuts and oily fruits are highly consumed worldwide due to their nutritional value and health benefits. However, the lipidomic analysis of these foods is still in its early stages.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Biochemical Research Methods
Stefano Bonciarelli, Bruna Neves, Pedro Domingues, Tania Melo, Laura Goracci, Maria Rosairio Domingues
Summary: Phosphatidylinositols (PIs) are complex lipids that can be modified by reactive oxygen and nitrogen species (RNS) to produce a variety of modified lipid products. This study used mass spectrometry to characterize the fragmentation signature of nitrated and nitroxidized PIs, allowing for the identification of these modified lipid products accurately. The findings of this study provide new opportunities to understand the pathophysiological roles of nitrated and nitroxidized PI derivatives at the cellular and tissue levels.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2023)
Article
Chemistry, Medicinal
Mariana Abraul, Artur Alves, Sandra Hilario, Tania Melo, Tiago Conde, Maria Rosario Domingues, Felisa Rey
Summary: Marine environments cover over 70% of the earth's surface and contain diverse habitats. Marine organisms are a valuable source of bioactive compounds with various health benefits. This study focused on the fatty acid profile and bioactive potential of lipid extracts from the fungi Emericellopsis cladophorae and Zalerion maritima, demonstrating their anti-inflammatory, antioxidant, and antibacterial properties.
Article
Chemistry, Medicinal
Ana S. P. Moreira, Diana Gaspar, Sonia S. Ferreira, Alexandra Correia, Manuel Vilanova, Marie-Mathilde Perrineau, Philip D. Kerrison, Claire M. M. Gachon, Maria Rosario Domingues, Manuel A. Coimbra, Filipe M. Coreta-Gomes, Claudia Nunes
Summary: This study aimed to characterize the chemical structure of water-soluble polysaccharides from Saccharina latissima and evaluate their immunostimulatory and hypocholesterolemic activities. It was found that laminarans and two fractions of fucose-containing sulphated polysaccharides showed immunostimulatory activity on B lymphocytes, and one of the fractions exhibited a significant effect in reducing cholesterol bioaccessibility.
Article
Food Science & Technology
Ana M. Peixoto, Silvia Petronilho, M. Rosario Domingues, Fernando M. Nunes, Joana Lopes, Marit Kvalvag Pettersen, Magnhild S. Grovlen, Elin M. Wetterhus, Idalina Goncalves, Manuel A. Coimbra
Summary: The brownish water-soluble extract (BrE) from the potato chip industry, which is usually landfilled, was found to have the potential to be used in active packaging. When incorporated into starch-based films, BrE improved their properties such as color, transparency, traction resistance, elasticity, antioxidant activity, hydrophilicity, water vapor barrier, and UV-light barrier. Moreover, the packaging film containing BrE showed better preservation effects on sliced cheese compared to the commonly used polyamide/polyethylene film.
Review
Biology
Tania Fernandes, M. Rosario Domingues, Paula I. Moreira, Claudia F. Pereira
Summary: Neurodegenerative diseases affect millions of people worldwide, but currently there is no cure for these disorders. Research on the endoplasmic reticulum-mitochondria interaction, called mitochondria-associated membranes (MAMs), is gaining attention in understanding the role of lipid metabolism in neurodegeneration. MAMs are involved in lipid synthesis, transport, and degradation, and changes in lipid metabolism can contribute to the development of neurodegenerative diseases. This review highlights the importance of lipid metabolism and MAMs in neurodegenerative disorders.
Article
Biochemistry & Molecular Biology
Anna Stasiewicz, Tiago Conde, Agnieszka Gegotek, Maria Rosario Domingues, Pedro Domingues, Elzbieta Skrzydlewska
Summary: The lipid extract from Nannochloropsis oceanica effectively protects human keratinocytes from UVB-induced damage by inhibiting cell death, reducing oxidative enzyme activity and ROS levels, and enhancing antioxidant enzyme activity. It also silences overexpressed membrane transporters caused by UVB radiation and decreases inflammation levels, stabilizing the natural metabolism of skin cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Felisa Rey, Paulo Cartaxana, Sonia Cruz, Tania Melo, M. Rosario Domingues
Summary: Marine algae are important sources of high-value compounds. This study focused on the lipidome and pigment profile of Acetabularia acetabulum, a macroscopic green single-celled alga. Analysis identified 16 polar lipid classes and 191 lipid species, as well as carotenoids and chlorophylls. The lipid extracts showed high antioxidant activity.
JOURNAL OF PHYCOLOGY
(2023)
Article
Biochemical Research Methods
Helena Beatriz Ferreira, Tania Melo, Ines M. S. Guerra, Ana S. P. Moreira, Paula Laranjeira, Artur Paiva, Laura Goracci, Stefano Bonciarelli, Pedro Domingues, M. Rosario Domingues
Summary: Autoimmune diseases like SLE and SS involve immune system dysregulation. Lipids have potential as biomarkers for improved diagnosis and prediction in these diseases. This study identified differences in lipid profiles in SLE and SS patients, including potential biomarkers like sphingomyelin and ceramide in SS and phosphatidylserine in SLE. Further research is needed to understand the role of lipids in the pathological mechanisms of these diseases.
JOURNAL OF PROTEOME RESEARCH
(2023)
Review
Biochemistry & Molecular Biology
Dmitry D. Evtyugin, Dmitry V. Evtuguin, Susana Casal, Maria Rosario Domingues
Summary: Plant sterols (PS) are cholesterol-like terpenoids widely spread in the kingdom Plantae. They have beneficial effects in healthy subjects and applications in various industries. However, there are still gaps in research on PS, and further studies are needed.
Article
Oncology
Sandrina Nobrega-pereira, Francisco Santos, Miguel Oliveira Santos, Teresa L. Serafim, Ana Patricia Lopes, Diogo Coutinho, Filipa S. Carvalho, Rosario M. Domingues, Pedro Domingues, Bruno Bernardes de Jesus, Vanessa A. Morais, Sergio Dias
Summary: Most cancer-related deaths are caused by metastases. A study showed that low-density lipoprotein (LDL)-cholesterol, a systemic factor, promotes the migration and invasion of triple-negative breast cancer (TNBC) cells by inducing the accumulation of lipid droplets and the expression of CD36. LDL also increases mitochondrial mass and network spread in migrating cells, leading to the dependence on fatty acids for mitochondrial respiration. Furthermore, LDL treatment increases reactive oxygen species (ROS) production. Blocking CD36 or ROS reverses the LDL-induced cell migration and metabolic adaptations. This research reveals a new vulnerability in metastatic breast cancer driven by LDL-induced reprogramming of mitochondrial metabolism.
CANCER RESEARCH COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Michal Biernacki, Tiago Conde, Anna Stasiewicz, Arkadiusz Surazynski, Maria Rosario Domingues, Pedro Domingues, Elzbieta Skrzydlewska
Summary: This study found that the lipid extract derived from Nannochloropsis oceanica microalgae can mitigate the detrimental effects of UVB radiation on skin cells by increasing the concentration of anti-inflammatory and antioxidant polyunsaturated fatty acids, and reducing the production of pro-inflammatory substances and the expression of receptors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
