Article
Food Science & Technology
Pufan Zheng, Mei Zhang, Xin Fang, Lili Tang, Zhixue Wang, Fuchen Shi
Summary: This study identified and quantified metabolites in three pear species using a targeted LC-MS/MS approach. The results showed distinct differences in metabolite composition between cultivars, and identified metabolic pathways related to fruit quality. D-xylose, formononetin, procyanidin A1 and beta-nicotinamide mononucleotide were identified as major differentially expressed metabolites.
Article
Environmental Sciences
Fabio Varriale, Luciana Tartaglione, Sevasti-Kiriaki Zervou, Christopher O. Miles, Hanna Mazur-Marzec, Theodoros M. Triantis, Triantafyllos Kaloudis, Anastasia Hiskia, Carmela Dell'Aversano
Summary: Cyanobacteria produce various bioactive secondary metabolites, including cyanotoxins, which are a global threat to humans and other organisms. Mass spectrometry-based methods have been developed for the determination of microcystins, the most studied class of cyanotoxins. This study compares targeted and untargeted liquid chromatography-mass spectrometry approaches for analyzing cyanobacterial biomass. It also describes the implementation of an analytical workflow for the identification of known and discovery of new cyanopeptides. The study reports the elucidation of new structural variants of microcystins and the proposal of structures for new cyanopeptides.
Article
Chemistry, Applied
Shi Shen, Xi Chen, Qin Zhuo, Yan Ma, Jingbo Wang, Liyuan Wang, Zhaolong Gong, Junsheng Huo
Summary: By applying untargeted and targeted metabolite analysis, differences in metabolites and compound contents among kiwifruits from different cultivars were found, which can benefit scientific research and new cultivar breeding.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2022)
Article
Plant Sciences
Francesco Sgadari, Antonietta Cerulli, Rosario Schicchi, Natale Badalamenti, Maurizio Bruno, Sonia Piacente
Summary: The wild and cultivated Capparis spinosa and Capparis orientalis in Sicily exhibit ecological and morphological differences. This study aimed to quantify the amount of quercetin in C. spinosa and C. orientalis by LC-ESI/QTrap/MS/MS and correlate it with pedoclimatic features. The results showed that quercetin is more abundant in C. orientalis compared to C. spinosa. The highest amounts of quercetin were found in flowers, leaves, and flower buds of C. orientalis from volcanic islands with southwest and east warm exposures. These findings provide a basis for further scientific investigations to explore the potential use of these plants as a source of quercetin for food or pharmaceutical purposes.
Article
Agriculture, Multidisciplinary
Xinran Wang, Yi Li, Lanzhen Chen, Jinhui Zhou
Summary: A comprehensive LC-MS-based metabolomics approach was developed to distinguish honey from Apis mellifera and Apis cerana. Untargeted and targeted strategies were used to identify potential markers for authentication. The study highlights the importance of these markers for differentiating between the two types of honey.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Food Science & Technology
Rafaela Prata, Rosalia Lopez-Ruiz, Mateus Henrique Petrarca, Helena Teixeira Godoy, Antonia Garrido Frenich, Roberto Romero-Gonzalez
Summary: In this study, a quick and effective analysis method was applied to monitor pesticides and aflatoxins in baby food marketed in Brazil. The results indicated the presence of pesticide residues in the samples, which could pose potential risks to infant health. In addition, suspect screening analysis revealed the presence of other contaminants.
Article
Endocrinology & Metabolism
Ivayla Roberts, Marina Wright Muelas, Joseph M. Taylor, Andrew S. Davison, Yun Xu, Justine M. Grixti, Nigel Gotts, Anatolii Sorokin, Royston Goodacre, Douglas B. Kell
Summary: This study utilizes untargeted metabolomics analysis to evaluate 120 samples of COVID-19 patients and predict the severity and outcome of the infection. The study identifies a group of metabolites with relevant biological functions, such as deoxycytidine and ureidopropionate (indirectly reflecting viral load), kynurenine (reflecting host inflammatory response), and multiple short chain acylcarnitines (energy metabolism) that can predict disease severity and outcome.
Article
Microbiology
Pierluigi Reveglia, Maria Luisa Raimondo, Marco Masi, Alessio Cimmino, Genoveffa Nuzzo, Gaetano Corso, Angelo Fontana, Antonia Carlucci, Antonio Evidente
Summary: This study used LC-MS/MS metabolomics to investigate the secondary metabolites produced by Phaeoacremonium species and their phytotoxicity. The toxic effects varied among fungal isolates, and the amounts of isosclerone and scytalone also differed.
Article
Agriculture, Multidisciplinary
Guo Lin-hui, Ge Da-peng, Ren Yuan, Dong Jian-mei, Zhao Xue-qing, Liu Xue-qing, Yuan Zhao-he
Summary: Secondary metabolites in pomegranates are closely related to nutritional quality and health functions. A study on wild and cultivated pomegranate plants in China revealed significant differences in flavonoid and tannin contents among different accessions. Latitude and altitude were identified as main environmental factors affecting the content of secondary metabolites. Over 200 secondary metabolites were identified in pomegranates, with 90 differential metabolites identified in two specific parts of the plant. This study provides valuable insights for utilizing wild pomegranate resources and breeding programs in China.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2022)
Article
Food Science & Technology
Jing Wan, Peng He, Yuanshan Chen, Qiujin Zhu
Summary: A practical, efficient, and rapid method for quantifying 19 pyrethroids in tea and orange samples was developed. Out of 262 tea samples, 11 pyrethroids were detected positive with concentrations below maximum residue limits. No pyrethroid was found in 209 peeled orange samples.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Chemistry, Analytical
Pengfei Li, Xi Zhang, Shumin Wang, Jing Wang, Ziqi Liu, Chen Wang, Weihang Tong, Lihong Liu
Summary: Letrozole, a third generation aromatase inhibitor, is suitable for the treatment of breast cancer. A rapid and reliable LC-MS/MS method was developed to determine letrozole concentration in human plasma, allowing evaluation of its clinical efficacy and adverse reactions. This method was sensitive and accurate, making it suitable for clinical pharmacokinetic and therapeutic drug monitoring.
ACTA CHROMATOGRAPHICA
(2022)
Article
Ophthalmology
Ai Lin Daphne Teh, Jaime Jacqueline Jayapalan, Mun Fai Loke, Azida Juana Wan Abdul Kadir, Visvaraja Subrayan
Summary: This study used a non-targeted metabolomics approach to investigate metabolite differences between patients with keratoconus and control subjects, identifying potential serum biomarkers. The identified metabolites, including dehydroepiandrosterone sulfate and eicosanoids, showed significant upregulation in keratoconus patients. Glycerophospholipid PS(17:2(9Z,12Z)/20:4 (5Z,8Z,11Z,14Z)) was significantly upregulated in control subjects. These differentially regulated metabolites provide insights into the pathophysiology of keratoconus and could potentially be utilized as biomarkers for early diagnosis and monitoring of the disease.
EXPERIMENTAL EYE RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Dmitrii Smirnov, Pavel Mazin, Maria Osetrova, Elena Stekolshchikova, Ekaterina Khrameeva
Summary: Lipidomics is a rapidly growing discipline that involves the identification and quantification of thousands of lipids. This paper provides guidelines for analyzing lipidome data obtained using untargeted LC-MS methods, focusing on practical approaches for data analysis. The paper also outlines potential applications of untargeted lipidomics for biological studies and includes a detailed R notebook for data analysis based on xcms software.
Article
Biochemistry & Molecular Biology
Andrea Montis, Florence Souard, Cedric Delporte, Piet Stoffelen, Caroline Stevigny, Pierre Van Antwerpen
Summary: This study revealed significant differences in the metabolite profiles of Coffea anthonyi compared to other coffee species, with lower levels of caffeine and higher levels of caffeoylquinic acid and mangiferin-related compounds in its leaves and fruits. Coffea anthonyi could be a promising candidate for the development of innovative healthcare products made with coffee extracts.
Article
Food Science & Technology
Larissa Marina Pereira Silva, Maria Raquel Cavalcanti Inacio, Gualter Guenter Costa da Silva, Jucier Magson de Souza e Silva, Jefferson Romaryo Duarte da Luz, Maria das Gracas Almeida, Edgar Perin Moraes, Debora Esposito, Leandro De Santis Ferreira, Silvana Maria Zucolotto
Summary: Moringa oleifera leaves are rich in flavonoids, and the highest flavonoid content can be obtained through ultrasound-assisted extraction. The optimal cultivation condition is using mineral soil fertilizer in the drought season, with the optimized extraction conditions being 80% ethanol and 13.4 minutes extraction time. The extract is safe and significantly reduces inflammation, with major compounds derived from apigenin, quercetin, and kaempferol glycosides.
Article
Chemistry, Analytical
Gilda D'Urso, Paola Montoro, Sonia Piacente
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2020)
Article
Biochemistry & Molecular Biology
Gilda D'Urso, Jurriaan J. Mes, Paola Montoro, Robert D. Hall, Ric C. H. de Vos
Article
Multidisciplinary Sciences
Manuela Grimaldi, Angelica Palisi, Carmen Marino, Paola Montoro, Anna Capasso, Sara Novi, Mario Felice Tecce, Anna Maria D'Ursi
Article
Chemistry, Analytical
Alfredo Bottone, Paola Montoro, Milena Masullo, Cosimo Pizza, Sonia Piacente
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2020)
Article
Biochemistry & Molecular Biology
Paola Montoro, Gilda D'Urso, Adam Kowalczyk, Carlo Ignazio Giovanni Tuberoso
Summary: Through various complex analytical methods, the bitter component in strawberry tree honey was identified as unedone. This study provides important clues for identifying specific flavor substances in honey.
Article
Biochemistry & Molecular Biology
Monica R. Loizzo, Rosa Tundis, Mariarosaria Leporini, Gilda D'Urso, Rossella Gagliano Candela, Tiziana Falco, Sonia Piacente, Maurizio Bruno, Francesco Sottile
Summary: This study presents a model for reusing almond skin to formulate blackberry jam and investigates its phytochemical profile and bioactivity through various methods. The enriched jam shows antioxidant, hypoglycemic, and hypolipidemic effects, with increased activity after thermal processing.
Article
Food Science & Technology
Maria Assunta Crescenzi, Gilda D'Urso, Sonia Piacente, Paola Montoro
Summary: This study evaluated the potential of using food waste as a source of bioactive metabolites through metabolomic analysis, focusing on fennel waste and identifying various beneficial compounds with anti-inflammatory, antioxidant, and/or immunomodulatory properties. The presence of unique metabolites, such as oxylipins, in fennel waste was also discovered. These findings suggest that food waste could serve as a valuable resource for producing cosmetic and nutraceutical products with bioactive compounds.
Article
Horticulture
L. d'Aquino, B. Lanza, E. Gambale, M. Sighicelli, P. Menegoni, G. C. Modarelli, J. Rimauro, E. Chianese, G. Nenna, T. Fasolino, G. D'Urso, S. Piacente, P. Montoro
Summary: This study simulated market-oriented cultivation of basil using a new-concept microcosm device. The results showed that the device supported the growth and biomass yield of basil, and different light conditions affected plant growth and yield.
SCIENTIA HORTICULTURAE
(2022)
Article
Biochemistry & Molecular Biology
Maria Assunta Crescenzi, Gilda D'Urso, Sonia Piacente, Paola Montoro
Summary: Fennel waste, particularly the little stem and leaf, contains bioactive compounds with promising anti-inflammatory activities. Phenolic acids and glycosylated flavonoids are key metabolites that have the ability to inhibit COX-2. Fennel leaf, therefore, should be included in nutraceuticals or functional foods for human consumption due to its potential health benefits.
Article
Biochemistry & Molecular Biology
Maria Assunta Crescenzi, Gilda D'Urso, Sonia Piacente, Paola Montoro
Summary: Seasonality is a crucial environmental factor affecting the metabolome of a plant, thereby leading to variations in the content of metabolites and their biological properties. This study quantified biologically active compounds in F. vulgare waste obtained during different seasons and found that the summer-grown Preludio variety had a higher content of bioactive compounds, resulting in better antioxidant power.
Article
Biochemistry & Molecular Biology
Angela Fadda, Paola Montoro, Gilda D'Urso, Nicoletta Ravasio, Federica Zaccheria, Daniele Sanna
Summary: Bio-based, non-toxic and biodegradable solvents and mechanical extraction were used to extract phenolic-rich oils from myrtle seeds. The oils had different yields, peroxide values, lipid compositions, and total phenolic concentrations depending on the extraction methods and myrtle varieties. The oxidative stability of the oils and the role of phenolic compounds in radical species evolution were also studied.
Article
Plant Sciences
Luigi d'Aquino, Rosaria Cozzolino, Giovanni Nardone, Gianni Borelli, Emilia Gambale, Maria Sighicelli, Patrizia Menegoni, Giuseppe Carlo Modarelli, Juri Rimauro, Elena Chianese, Giuseppe Nenna, Tommaso Fasolino, Gilda D'Urso, Paola Montoro
Summary: The indoor cultivation of basil using artificial lighting is gaining interest. This study compared the effects of white and blue-red light on plant growth, photochemistry, nutrient concentration, and secondary metabolism. The plants grown under white light showed better growth and biomass production compared to those grown under blue-red light. Both light conditions had different effects on nutrient concentration and translocation rate, and no photosynthetic stress was observed. However, leaves grown under white light had higher levels of maximum quantum yield of PSII and electron transport rate. Differences in metabolic patterns, especially phenolic compound concentrations, were detected under the two lighting conditions.