Review
Food Science & Technology
Meijun Zeng, Jan-Peter van Pijkeren, Xuejun Pan
Summary: Prebiotics are used to modulate gut microbiota and improve host health. Gluco-oligosaccharides (GlcOS) have been found to exert prebiotic potentials due to their selective fermentation by beneficial gut bacteria. However, the prebiotic effects of GlcOS vary due to their complex structure. This review provides an overview of GlcOS as potential prebiotics, covering their synthesis, purification, structural characterization, and prebiotic effect evaluation.
COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY
(2023)
Review
Microbiology
Yantyati Widyastuti, Andi Febrisiantosa, Flavio Tidona
Summary: Lactobacillus bacteria have long been used in food fermentation, particularly in the production of high-value functional dairy products like cheese and yogurt. These bacteria play a role in releasing bioactive peptides from milk protein, which can have positive impacts on human health.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Despoina Eugenia Kiousi, Nikos Chorianopoulos, Chrysoula C. Tassou, Alex Galanis
Summary: Food fermentation has improved the safety and organoleptic characteristics of raw materials, and the potential health benefits of these products have attracted scientific attention. However, only a few products have been authorized as 'functional foods' due to data inconsistency and lack of in-depth preclinical characterization. Omics platforms and bioinformatic algorithms offer comprehensive data analysis of microbe-microbe, microbe-matrix, and microbe-host interactions, providing insights for maximizing their beneficial effects. Integrating these platforms into food science remains a challenge, but coordinated efforts and interdisciplinary collaboration can drive the field towards a new era.
Article
Multidisciplinary Sciences
Louise P. Ramos, Milena E. De Almeida, Herbert P. S. Freire, Wallace F. B. Pessoa, Rachel P. Rezende, Carla C. Romano
Summary: This study discovered a strain of Lactiplantibacillus plantarum 6.2 isolated from cocoa fermentation, which exhibited strong antagonistic activity against Gardnerella vaginalis, possibly through the action of proteinaceous bacteriocins. The lactobacilli and its supernatant were also able to exclude and displace G. vaginalis from adhesion sites on vaginal epithelial cells. Therefore, this strain shows potential as a good probiotic for the treatment of bacterial vaginosis and as a complement to existing therapies.
ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS
(2022)
Article
Biotechnology & Applied Microbiology
Sofia Fernandez-Ciganda, Martin Fraga, Pablo Zunino
Summary: The study evaluated changes in the fecal microbiota of calves after the administration of two probiotic lactobacilli strains, finding that modulation of the intestinal microbiota is a potential mechanism behind the beneficial effects of these probiotic strains.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2022)
Article
Microbiology
Margherita D'Alessandro, Carola Parolin, Silvia Patrignani, Gilda Sottile, Patrizio Antonazzo, Beatrice Vitali, Rosalba Lanciotti, Francesca Patrignani
Summary: This study focuses on the isolation and characterization of lactobacilli/bifidobacteria from human breast milk to find new probiotic candidates for food products. The breast milk strains demonstrate good adhesion to intestinal cells, antagonistic activity against food spoilage and pathogenic species, and the potential to release aroma compounds associated with sensory quality of dairy products.
Review
Food Science & Technology
Yun Lu, Shuqi Xing, Laping He, Cuiqin Li, Xiao Wang, Xuefeng Zeng, Yifeng Dai
Summary: Lactobacilli have been widely studied and employed as functional starter cultures in the food industry. Various molecular techniques are used for identification and evaluation, and efforts are being made to enhance performance, improve cell density, and develop production techniques for directed vat set (DVS) starter of Lactobacilli strains. Challenges in the viability and stability of Lactobacilli at an industrial scale are being addressed, and new production equipment and technology have the potential for large-scale application.
Article
Food Science & Technology
Margherita D'Alessandro, Davide Gottardi, Carola Parolin, Virginia Teresa Glicerina, Beatrice Vitali, Rosalba Lanciotti, Francesca Patrignani
Summary: Human microbial niches, such as the healthy vagina, have recently been recognized as alternative sources of probiotics for preventing vaginal diseases. These microorganisms can be administered orally and reach the vaginal environment by passing through the gastrointestinal tract. This study developed and characterized fermented soy beverages containing encapsulated and non-encapsulated vaginal lactobacilli as potential dietary strategies for treating vaginal dysbiosis. The viability of the strains remained stable during storage, and samples with encapsulated bacteria showed improved water holding capacity, lactic acid content, and antagonistic activity against enteropathogens. However, encapsulation reduced the acceptability of the final products.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Food Science & Technology
Ville M. Koistinen, Maria Hedberg, Lin Shi, Anders Johansson, Otto Savolainen, Marko Lehtonen, Anna-Marja Aura, Kati Hanhineva, Rikard Landberg
Summary: This study used metabolomics to identify seven microbial metabolites commonly produced during fermentation and found that a mixture of these metabolites exhibited stronger inhibition against bacteria compared to individual compounds. The results suggest that metabolites produced by LAB may modulate local microbial ecology through synergistic effects.
MOLECULAR NUTRITION & FOOD RESEARCH
(2022)
Article
Multidisciplinary Sciences
Jingyan Zhang, Kailing Li, Xinping Bu, Shumin Cheng, Zhi Duan
Summary: A novel strain of lactic acid bacteria, named Lacticaseibacillus rhamnosus VHProbi M14, was isolated from cheese and showed strong growth inhibitory effects on Streptococcus mutans and Fusobacterium nucleatum. This strain coaggregated with the pathogenic bacteria, reduced their adhesion on human primary gingival epithelial cells, and produced antibacterial substances with protein- and peptide-like structures. The M14 strain contained 23 bacteriocin-related genes and exhibited inhibitory effects on 8 other pathogenic strains, suggesting its potential as a probiotic bacterium for clinical applications.
Article
Biotechnology & Applied Microbiology
Glauber Campos Vale, Brenda Izabela Santana Mota, Ellen Sayuri Ando-Suguimoto, Marcia Pinto Alves Mayer
Summary: This study aimed to evaluate the antibacterial gene expression of dendritic cells challenged with LPS and probiotics. The results showed that LPS upregulated more genes related to inflammation and immune response compared to the groups where probiotics were added. Although probiotics decreased several genes upregulated by LPS, they still upregulated some genes encoding cytokines. Additionally, one specific probiotic promoted the secretion of these proteins.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2023)
Article
Veterinary Sciences
Feriel Yasmine Mahiddine, Inhwan You, Heekee Park, Min Jung Kim
Summary: This study investigated the effects of oral administration of three commensal Lactobacillus spp. on the sperm quality of healthy dogs. The results showed that supplementation with commensal lactobacilli significantly improved the qualitative parameters and motility of sperm. Furthermore, the study revealed a positive correlation between the regulation of gut microbiota and sperm quality.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Silvia-Simona Grosu-Tudor, Iulia-Roxana Angelescu, Alexandru Brinzan, Medana Zamfir
Summary: S-layer proteins produced by lactobacilli isolated from Romanian artisan fermented products were characterized. The presence of S-layer proteins was confirmed by electron microscopy, and their removal caused a loss of bacterial resistance and self-aggregation ability. Liquid chromatography-mass spectrometry analysis identified the molecular masses and isoelectric points of the proteins. PCR amplification revealed the similarity between the genome of Lact. helveticus 34.9 and slpA gene.
JOURNAL OF APPLIED MICROBIOLOGY
(2023)
Article
Microbiology
Madison A. R. Rothwell, Yawen Zhai, Christian G. Pagan-Medina, Ilenys M. Perez-Diaz
Summary: This study investigated the growth of γ-proteobacteria in fresh cucumber juice, fermentation cover brine, and cucumber fermentation. Results showed that while fresh cucumbers provide nutrients for γ-proteobacteria growth, they are unable to proliferate in cucumber fermentation.
MICROBIOLOGY SPECTRUM
(2022)
Article
Food Science & Technology
Cesar R. Balcazar-Zumaeta, Alexa J. Pajuelo-Munoz, Deisy F. Trigoso-Rojas, Angel F. Iliquin-Chavez, Editha Fernandez-Romero, Ives Yoplac, Lucas D. Munoz-Astecker, Nadia Rodriguez-Hamamura, Ily M. Maza Mejia, Ilse S. Cayo-Colca, Gilson C. A. Chagas-Junior, Jorge L. Maicelo-Quintana, Efrain M. Castro-Alayo
Summary: This study demonstrated the importance of reducing fermentation time in cocoa beans to obtain fermented beans of consistent quality. The use of a starter culture was found to release more compounds, especially alcohols and esters, in a shorter time. Therefore, it is necessary to shorten the fermentation time in the Amazonas region.
Article
Biotechnology & Applied Microbiology
M. R. S. Ribeiro, D. R. Oliveira, M. V. Caliari, D. C. Cara Machado, M. E. R. Andrade, V. N. Cardoso, F. dos Santos Martins, J. R. Nicoli, M. A. Gomes
Summary: This study evaluated the effect of treatment with the probiotic Saccharomyces boulardii with or without metronidazole in experimental giardiasis, finding a significant reduction in parasitic load and protection against intestinal mucosal damage and oxidative stress. The results suggest the potential use of S. boulardii as an alternative treatment for giardiasis, especially in cases of resistance or intolerance to conventional treatment.
JOURNAL OF APPLIED MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Camila Francisco Moreira, Puebla Cassini-Vieira, Maria Cecilia Campos Canesso, Mariane Felipetto, Hedden Ranfley, Mauro Martins Teixeira, Jacques Robert Nicoli, Flaviano Santos Martins, Luciola Silva Barcelos
Summary: The study showed that oral treatment with Lactobacillus rhamnosus accelerates skin wound closure, reduces scar formation, alleviates inflammation and fibrogenesis, and improves angiogenesis in wounded skin.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2021)
Article
Biotechnology & Applied Microbiology
Nathan das Neves Selis, Hellen Braga Martins de Oliveira, Yan Bento dos Anjos, Hiago Ferreira Leao, Beatriz Almeida Sampaio, Thiago Macedo Lopes Correia, Mariane Mares Reis, Thamara Louisy Santos Brito, Carolline Florentino Almeida, Larissa Silva Carvalho Pena, Lais Ferraz Brito, Roberta Maria Ornelas, Tiza Teles Santos, Guilherme Barreto Campos, Jorge Timenetsky, Mariluze Peixoto Cruz, Andrea Miura da Costa, Regiane Yatsuda, Ana Paula Trovatti Uetanabaro, Lucas Miranda Marques
Summary: This study evaluated the potential probiotic effects of five Lactobacillus strains isolated from cupuacu fruit fermentation against vaginal pathogens. The results demonstrated the ability of the lactobacilli to inhibit the growth of Gardnerella vaginalis and Neisseria gonorrhoeae, indicating their potential as probiotics for vaginal health. Additionally, the strains showed biofilm production, antimicrobial activity, and the production of lactic acid as the most abundant organic acid, suggesting their promising application in supporting vaginal health.
BIOMED RESEARCH INTERNATIONAL
(2021)
Article
Biotechnology & Applied Microbiology
Joana O. P. A. Coutinho, Taynara S. Peixoto, Graciele C. A. de Menezes, Camila R. Carvalho, Mayara B. Ogaki, Eldon C. Q. Gomes, Carlos A. Rosa, Luiz H. Rosa, Rosa M. E. Arantes, Jacques R. Nicoli, Fabiana C. P. Tiago, Flaviano S. Martins
Summary: This study aimed to screen putative probiotics among 254 yeasts isolated from Antarctica, and two yeasts, Rhodotorula mucilaginosa UFMGCB 18377 and Saccharomyces cerevisiae UFMGCB 11120, were selected for in vivo evaluation using mice challenged with S. Typhimurium. The results indicated that R. mucilaginosa UFMGCB 18377 presented probiotic potential by protecting mice against Salmonella infection. This is the first study to screen yeasts from Antarctic environments and of Rhodotorula genus for probiotic use.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2021)
Article
Genetics & Heredity
Jessica Gabrielle Vidal da Silva, Angelica Thomaz Vieira, Thiago J. Sousa, Marcus Vinicius Canario Viana, Doglas Parise, Bruna Sampaio, Alessandra Lima da Silva, Luis Claudio Lima de Jesus, Pedro Kassio Ribeiro Matos Loureiro de Carvalho, Leticia de Castro Oliveira, Flavia Figueira Aburjaile, Flaviano S. Martins, Jacques Robert Nicoli, Preetam Ghosh, Bertram Brenig, Vasco Azevedo, Anne Cybelle Pinto Gomide
Summary: The Bifidobacterium longum 5(1A) strain isolated from a healthy child's feces has probiotic properties attributed to its production of metabolites such as acetate. Comparative genomics identified unique genes possibly related to acetate production, highlighting potential benefits to host health. The strain's distinct genes suggest the use of different carbohydrate sources for greater acetate production.
Article
Biotechnology & Applied Microbiology
Raphael S. Steinberg, Lilian C. Silva e Silva, Marcelo R. de Souza, Ronaldo B. Reis, Adriano F. Bicalho, Joao P. S. Nunes, Adriana A. M. Dias, Jacques R. Nicoli, Elisabeth Neumann, Alvaro C. Nunes
Summary: This study successfully isolated lactic bacteria with potential for combating mastitis, showing strong antagonism against mastitis pathogens and the ability to produce H2O2. These findings contribute to the future development of new tools for the prevention and treatment of bovine mastitis.
INTERNATIONAL MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Joana O. P. A. Coutinho, Monica F. Quintanilha, Marina R. A. Campos, Enio Ferreira, Graciele C. A. de Menezes, Luiz H. Rosa, Carlos A. Rosa, Katia D. Vital, Simone O. A. Fernandes, Valbert N. Cardoso, Jacques R. Nicoli, Fabiana C. P. Tiago, Flaviano S. Martins
Summary: Mucositis, a severe side effect of chemotherapy drugs in cancer treatment, has no effective treatment currently. This study showed that oral administration of the Antarctic strain of Rhodotorula mucilaginosa UFMGCB 18,377 could effectively reduce mucositis induced by 5-FU.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2022)
Article
Biotechnology & Applied Microbiology
Spencer S. Santos, Vivian C. Miranda, Luisa M. Trindade, Valbert N. Cardoso, Diego C. Reis, Geovanni D. Cassali, Jacques R. Nicoli, Denise C. Cara, Flaviano S. Martins
Summary: This study evaluated the probiotic effect of Bifidobacterium longum subsp. longum 5(1A) in a murine model of food allergy. The results showed that oral administration of this probiotic prevented weight loss, reduced immune reactions, and improved intestinal permeability. The dose of 10^8 CFU showed the best protective effect, and the use of live bacteria was necessary to achieve these beneficial effects.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2023)
Article
Food Science & Technology
Karen Costa, Luana S. Silva, Cintia N. Kobori, Andreia M. da Silva, Jacques R. Nicoli
Summary: The study showed that microencapsulation techniques can effectively improve the survival rate of probiotics, especially when incorporated into plant pulp powder. Acerola pulp matrix can be used as an alternative vehicle for probiotic administration, maintaining efficacy even when stored at room temperature.
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Medicine, Research & Experimental
Monica F. Quintanilha, Vivian C. Miranda, Ramon O. Souza, Bruno Gallotti, Clenio Cruz, Elandia A. Santos, Jacqueline Alvarez-Leite, Luis C. L. Jesus, Vasco Azevedo, Luisa M. Trindade, Valbert N. Cardoso, Enio Ferreira, Barbara A. Carvalho, Pedro M. G. Soares, Angelica T. Vieira, Jacques R. Nicoli, Flaviano S. Martins
Summary: This study demonstrates that Bifidobacterium longum 5(1A), a probiotic, has a protective effect against intestinal damage caused by irinotecan, suggesting its potential use as a probiotic therapy for mucositis.
Article
Biotechnology & Applied Microbiology
Quesia S. Damaceno, Bruno Gallotti, Isabela M. M. Reis, Yasmim C. P. Totte, Gabriella B. Assis, Henrique C. Figueiredo, Tales F. Silva, Vasco Azevedo, Jacques R. Nicoli, Flaviano S. Martins
Summary: Breast milk contains beneficial bacteria that can positively influence the health of newborns. The study identified 77 bacteria belonging to four genera, including Staphylococcus, Streptococcus, Leuconostoc, and Lacticaseibacillus. Two potential probiotics, Lacticaseibacillus rhamnosus and Leuconostoc mesenteroides, were selected and found to colonize the gastrointestinal tract in germ-free mice and maintain viability after spray-drying.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2023)
Correction
Multidisciplinary Sciences
Flaviano S. Martins, Guillaume Dalmasso, Rosa M. E. Arantes, Anne Doye, Emmanuel Lemichez, Patricia Lagadec, Veronique Imbert, Jean-Francois Peyron, Patrick Rampal, Jacques R. Nicoli, Dorota Czerucka
Article
Biotechnology & Applied Microbiology
Vivian C. Miranda, Ramon O. Souza, Monica F. Quintanilha, Bruno Gallotti, Helder C. Assis, Ana Maria C. Faria, Jacques R. Nicoli, Denise C. Cara, Flaviano S. Martins
Summary: This study aimed to evaluate the probiotic and postbiotic potential of Akkermansia muciniphila BAA-835 in an ovalbumin food allergy model. The results showed that treatment with viable A. muciniphila could alleviate weight loss, decrease serum levels of allergy-related antibodies, reduce intestinal injury and inflammation, and modify the gut microbiota composition. Additionally, the administration of inactivated bacteria also had a postbiotic effect by reducing allergy-related immune responses. These findings suggest that A. muciniphila BAA-835 has both probiotic and postbiotic properties in the prevention and treatment of food allergies.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2023)
Article
Biotechnology & Applied Microbiology
Diego Lisboa Rios, Patricia Costa Lima da Silva, Cesar Silva Santana Moura, Camila Nair Batista Couto Villanoeva, Gabriel da Rocha Fernandes, Ana Agustina Bengoa, Graciela Liliana Garrote, Analia Graciela Abraham, Jacques Robert Nicoli, Elisabeth Neumann, alvaro Cantini Nunes
Summary: This study compared the bacterial and fungal diversity of kefir beverages produced using milk or sugared water as propagation matrices. The results showed that milk and water kefir have different microbial communities, but share similar main functional processes with slight quantitative variations.
INTERNATIONAL MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Raphael S. Steinberg, Lilian C. Silva e Silva, Marcelo R. de Souza, Ronaldo B. Reis, Patricia C. L. da Silva, Gustavo A. Lacorte, Jacques R. Nicoli, Elisabeth Neumann, Alvaro C. Nunes
Summary: This study explored the bacterial diversity in raw milk samples from dairy cows using 16S rRNA amplicon sequencing. The milk bacterial microbiome was found to be dominated by Firmicutes, Proteobacteria, and Actinobacteria, with significant differences in composition related to udder health status and breed.
INTERNATIONAL MICROBIOLOGY
(2022)
