期刊
LWT-FOOD SCIENCE AND TECHNOLOGY
卷 73, 期 -, 页码 290-295出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.lwt.2016.06.026
关键词
Phospholipase A(2); Magnetic immobilization; Oil degumming; Free fatty acids
资金
- National Natural Science Foundation of China (NSFC) [31571880]
- Key Laboratory of Soybean Biology in Chinese Ministry of Education, Northeast Agricultural University, Harbin, China
In this study, magnetic immobilized phospholipase A(2) (PLA(2)) was used to degum soybean oil such as magnetic particles crosslinked with sodium alginate PLA(2) (PLA(2)-MPCSA), Magnetic chitosan micropartides containing PLA(2) (PLA(2)-MCM), Fe3O4/P(GMA-EDGM-St) PLA(2), and Fe3O4/SiOx-g-P(GMA) PLA(2), successfully improving the recycling aspect of the degumming process. The highest enzyme loading and enzymatic activity (122.60 mg/g and 1289 U/g, respectively) were obtained using the magnetic immobilized PLA(2)-Fe3O4/SiOx-g-P(GMA) system. Compared to free PLA(2), magnetic immobilized PLA(2) had a broader pH-activity profile (pH 4.0-5.5) and activity remained stable between 50 and 70 degrees C. Enzymatic degumming with PLA(2)-Fe3O4/SiOx-g-P(GMA) was carried out over 5.0 h with an enzyme dosage of 0.24 g/kg, a pH of 5.0, and a temperature of 55 degrees C, resulting in residual phosphorus and free fatty acid contents of 9.8 mg/kg and 0.84 g/100 g, respectively. Moreover, magnetic immobilized PLA(2) retained more than 80% of its initial activity even after 5 cycles of soybean oil degumming. (C) 2016 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据