期刊
LUNG CANCER
卷 100, 期 -, 页码 1-4出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.lungcan.2016.07.021
关键词
Non-small-cell lung cancer; KRAS mutation; Circulating tumor cells; Circulating tumor DNA; Monitoring of response
Liquid biopsies are a new non-invasive strategy to detect and monitor the biology of non-small-cell lung cancer (NSCLC) in the era of personalized medicine. KRAS is an oncogenic driver that is mutated in 30% of NSCLCs and is associated with a poor prognosis. 62 samples from 32 patients, treated for metastatic ICRAS-mutated lung adenocarcinoma, had DNA extracted from plasma and circulating tumor cells (CTC5) prospectively tested for the presence of KRAS mutations using droplet digital PCR. A KRAS mutation was detected in 82% of patients. Sensitivity was 78% for circulating free DNA (cfDNA) and 34% for CTCs. The presence of a KRAS mutation in cfDNA was correlated with a poor response to chemotherapy or targeted therapy. When a KRAS-mutated-DNA was detected and then monitored in cfDNA, its variation during targeted or conventional therapy was correlated with response, according to RECIST criteria, in 87.5% of cases (n = 14/16), whereas this correlation was observed in 37.5% of cases for CTCs (n = 3/8). We report the usefulness of using digital droplet PCR on liquid biopsies to predict and monitor responses to treatment of ICRAS-mutated lung adenocarcinoma. ctDNA was much more sensitive than CTC5 in this context. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
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