Multi-omics analysis identifies RFX7 targets involved in tumor suppression and neuronal processes

Recurrently mutated in lymphoid neoplasms, the transcription factor RFX7 is emerging as a tumor suppressor. Previous reports suggested that RFX7 may also have a role in neurological and metabolic disorders. We recently reported that RFX7 responds to p53 signaling and cellular stress. Furthermore, we found RFX7 target genes to be dysregulated in numerous cancer types also beyond the hematological system. However, our understanding of RFX7's target gene network and its role in health and disease remains limited. Here, we generated RFX7 knock-out cells and employed a multi-omics approach integrating transcriptome, cistrome, and proteome data to obtain a more comprehensive picture of RFX7 targets. We identify novel target genes linked to RFX7's tumor suppressor function and underscoring its potential role in neurological disorders. Importantly, our data reveal RFX7 as a mechanistic link that enables the activation of these genes in response to p53 signaling.

Automated summary

The transcription factor RFX7, which is frequently mutated in lymphoid neoplasms, has been identified as a tumor suppressor. It is also speculated to be involved in neurological and metabolic disorders. Recent research has shown that RFX7 is responsive to p53 signaling and cellular stress, with its target genes being dysregulated in various cancer types. However, the exact role of RFX7 in health and disease, as well as its target gene network, remains unclear.