Article
Fisheries
Eun Gyeong Lee, Jun Soung Kwak, Ki Hong Kim
Summary: In this study, a CRISPR/Cas9 system was used to produce auxotrophic E. piscicida mutants, which proved to be a simpler and faster method than the traditional allelic exchange method. The mutants showed superior protection against E. piscicida, but potential reversion was observed in some cases.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Xinya Zhang, Maocang Yan, Cuimin Mu, Jiayan Wu, Jiakang Chen, Guangchen Pan, Xuepeng Wang
Summary: Fucose regulates the pathogenicity of Edwardsiella piscicida by promoting its growth and mobility, while mutation of the fucP gene reduces the pathogenicity of the bacterium in zebrafish by affecting its penetration and motility in the intestine.
AQUACULTURE REPORTS
(2021)
Article
Fisheries
Feizi Hu, Zhuang Wang, Yuanxing Zhang, Dahai Yang, Qin Liu
Summary: The NF -KB signaling pathway is crucial for the innate immune response and defense against microbial infection. In this study, an in vivo NF -KB detection model was used in zebrafish larvae to investigate the molecular mechanism of NF -KB signaling in defense against E. piscicida infection. It was found that TLR5b activated the NF -KB pathway by recognizing the flagella of E. piscicida. Furthermore, the activation of NF -KB led to the upregulation of cxcl8a, which promoted the recruitment of neutrophils and reduced the colonization of E. piscicida, thereby improving the survival rate of larvae.
Article
Immunology
Banikalyan Swain, Cole T. T. Powell, Roy Curtiss
Summary: Vaccination is the most effective method for preventing and controlling infectious diseases in aquaculture. In this study, a genetically modified vaccine strain of Edwardsiella piscicida was created and tested in zebrafish. The vaccine strain induced immune responses and provided partial protection against the wild-type strain.
MICROBIAL PATHOGENESIS
(2022)
Article
Fisheries
Si Yao Zheng, Xinbin Shao, Zhitao Qi, Maocang Yan, Min Hui Tao, Xiao Man Wu, Lining Zhang, Jianzhong Ma, An Li, Ming Xian Chang
Summary: This study firstly demonstrates the functional characterization of zebrafish nos2a during bacterial infection, revealing its regulation of ROS and NO as well as the expression of proinflammatory cytokines, antibacterial genes, and oxidant factors. Furthermore, it finds that inhibition of NO can be effective in protecting against E. piscicida infection.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Cell Biology
Yunxing Liu, Fang Liang, Zijiong Dong, Song Li, Jianmin Ye, Wei Qin
Summary: ScCas9, derived from Streptococcus canis, shows editing activity in zebrafish and can induce mutations in the genome. Utilizing a ribonucleoprotein complex and synthetic modified crRNA:tracrRNA duplex, the editing ability of ScCas9 in zebrafish has been optimized for targeting relaxed NNG PAMs.
Article
Biotechnology & Applied Microbiology
Jose M. Uribe-Salazar, Gulhan Kaya, Aadithya Sekar, KaeChandra Weyenberg, Cole Ingamells, Megan Y. Dennis
Summary: Our study systematically assessed the accuracy of CRISPR/Cas9 tool predictions for on- and off-target gene editing in zebrafish embryos, revealing large discrepancies between experimentally observed editing efficiencies and predictions made by commonly used gRNA design tools. We also investigated potential confounders of G(0)-based CRISPR screens by analyzing control embryos, identifying potentially lasting effects from the microinjection process. This research provides a valuable resource for the zebrafish community to enhance the design and implementation of CRISPR/Cas9 experiments.
Article
Genetics & Heredity
Daichi Sahashi, Yurina Kubo, Mika Ishii, Asami Ikeda, Chiharu Yamasaki, Masaharu Komatsu, Kazuhiro Shiozaki
Summary: Neu1 sialidase plays a crucial role in regulating lysosomal degradation in macrophages, thus affecting the susceptibility of fish to bacterial infection.
Article
Fisheries
Jiuling Zhu, Jiahang Yang, He Wen, Mengtian Wang, Xiaojing Zheng, Junli Zhao, Xiaohong Sun, Peiyan Yang, Qinwen Mao, Yu Li, Haibin Xia
Summary: FAM76B is a nuclear speckle-localized protein highly expressed in the hematopoiesis and immune systems of zebrafish. Knockout of the fam76b gene in zebrafish resulted in reduced thymus, excessive inflammatory response, and increased mortality. These findings contribute to our understanding of the biological function of FAM76B and the development of zebrafish hematopoietic and immune systems.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Miriam Zink, Anne Seewald, Mareike Rohrbach, Andreas Brodehl, Daniel Liedtke, Tatjana Williams, Sarah J. Childs, Brenda Gerull
Summary: In this study, transgenic zebrafish models were used to investigate the pathogenesis of TMEM43-associated cardiomyopathy. The findings reveal that TMEM43 mutations lead to cardiomyocyte hypertrophy, cardiac morphological defects, and dysregulated gene expression. These results provide new insights into the development of TMEM43-associated cardiomyopathy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Genetics & Heredity
Emily P. Y. Yu, Sofia Perin, Vishal Saxena, Marc Ekker
Summary: By studying dlx gene mutants in zebrafish, it was found that the expression of dlx genes in zebrafish is influenced by different regulatory factors, which differs from studies in mice. This may highlight evolutionary differences in gene interactions between vertebrates.
Article
Fisheries
Abigail R. Armwood, Matt J. Griffin, Bradley M. Richardson, David J. Wise, Cynthia Ware, Alvin C. Camus
Summary: This study clarifies the pathology of E. tarda, E. piscicida, and E. anguillarum infections in farm-raised catfish in the US. The results show that E. piscicida causes the most severe pathology, while E. anguillarum and E. tarda only warrant minimal concern. Additionally, some cross-protective effects were observed among E. piscicida, E. anguillarum, and E. ictaluri.
JOURNAL OF FISH DISEASES
(2022)
Article
Fisheries
Tingqi Ye, Cuimin Mu, Jiakang Chen, Guangchen Pan, Xuepeng Wang
Summary: The regulatory protein UhpA in Edwardsiella piscicida plays a role in affecting the expression of key virulence genes, suggesting its involvement in the pathogenicity of the bacteria in fish. The uhpA mutant strain showed altered gene expression levels compared to wild strains, providing insights into the mechanism of virulence gene regulation.
JOURNAL OF FISH DISEASES
(2021)
Article
Fisheries
Wan-Ping Bian, Shao-Lin Xie, Chao Wang, Grigoriy G. Martinovich, Yan-Bo Ma, Pan-Pan Jia, De-Sheng Pei
Summary: By generating a mitfa knock-out zebrafish line, the study reveals the impact of mitfa on the immune system, with reduced melanin levels and decreased macrophage numbers in mitfa-/- zebrafish. Interestingly, the expression levels of immune-related genes were significantly increased in mitfa-/- zebrafish after bacterial challenge or bifenazate exposure. Xenograft experiments showed a low cell migration ratio, indicating highly activated immune system after loss of mitfa.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Zhuang Wang, Di Mu, Yu Jiang, Jing He, Yuanxing Zhang, Dahai Yang, Qin Liu
Summary: This study found that propolis has a protective effect on turbot against Edwardsiella piscicida infection. It can improve the expression of anti-oxidative stress related enzymes, alleviate histopathological changes, and increase the expression of cytokines. The results suggest that propolis may be a promising immunopotentiator candidate in aquaculture.
FISH & SHELLFISH IMMUNOLOGY
(2022)