Article
Cell Biology
Fatemeh Safi, Parashar Dhapola, Sarah Warsi, Mikael Sommarin, Eva Erlandsson, Jonas Ungerback, Rebecca Warfvinge, Ewa Sitnicka, David Bryder, Charlotta Boiers, Ram Krishna Thakur, Goran Karlsson
Summary: By investigating the chromatin accessibility of single cells, it has been found that there is a population of cells that display both stem-like and lineage-affiliated chromatin signatures. These cells are positioned between stem cells and committed progenitors, showing multi-lineage potential but lacking self-renewal activity.
Article
Cell & Tissue Engineering
Zhengqi Wang, Grace Emmel, Hong Seo Lim, Wandi Zhu, Astrid Kosters, Eliver E. B. Ghosn, Peng Qiu, Kevin D. Bunting
Summary: This study reveals the important role of STAT5ab gene in both hematopoietic and stromal cells, and the deletion of STAT5ab using specific Cre promoters leads to a reduction in multipotent hematopoietic progenitors. Furthermore, STAT5ab is involved in the secretion of niche factors in mesenchymal stem cells and the differentiation priming of hematopoietic stem cells.
Review
Developmental Biology
Suwei Gao, Yifan Zhang, Feng Liu
Summary: For a long time, it was believed that self-renewing and multipotent hematopoietic stem cells (HSCs) were the main contributors to both embryonic and adult hematopoiesis. However, recent studies have shown that native hematopoiesis is not solely driven by hierarchical differentiation of HSCs, but rather by hematopoietic progenitor cells (HPCs). These findings help redefine our understanding of the cellular origins of embryonic and adult hematopoiesis and highlight the differences in revealing HSC potential versus HSC fate using different approaches.
Article
Multidisciplinary Sciences
Leal Oburoglu, Els Mansell, Niels-Bjarne Woods
Summary: Glutamine metabolism plays an essential role in regulating the emergence and specification of hematopoietic cells during endothelial to hematopoietic transition (EHT), with different hematopoietic lineages requiring distinct derivatives of glutamine.
SCIENTIFIC REPORTS
(2021)
Article
Engineering, Biomedical
Pan Zhang, Linmu Xu, Jingsong Gao, Guangkui Xu, Yanping Song, Guang Li, Jingjing Ren, Yunjie Zhang, Cheng Yang, Yu Zhang, Ruiheng Xie, Nu Zhang, Hui Yang
Summary: This study investigates the behaviors of hematopoietic progenitor cells using collagen hydrogels of different stiffness and characterizes their transcriptional profiles through single-cell RNA sequencing. The results demonstrate that 3D collagen hydrogels promote the differentiation of progenitor cells into macrophages and enhance communication with other hematopoietic clusters.
BIOACTIVE MATERIALS
(2022)
Article
Biochemistry & Molecular Biology
Quan M. Phan, Lucia Salz, Sam S. Kindl, Jayden S. Lopez, Sean M. Thompson, Jasson Makkar, Iwona M. Driskell, Ryan R. Driskell
Summary: In this study, the researchers used multimodal single-cell approaches, epigenetic assays, and allografting techniques to investigate the differentiation mechanism of dermal fibroblast progenitors (DFPs) during skin development. They found that the overall transcription profile of DFPs is repressed by H3K27me3 and that they are unable to reform the skin in allograft assays. Further analysis revealed that the H3K27me3 demethylase, Kdm6b/Jmjd3, plays a role in derepressing DFPs during murine skin development. This study provides a multimodal understanding of DFP differentiation and offers a novel multiomics search tool.
Article
Oncology
Margot F. van Spronsen, Diana Hanekamp, Theresia M. Westers, Noortje van Gils, Eline Vermue, Arjo Rutten, Joop H. Jansen, Birgit I. Lissenberg-Witte, Linda Smit, Gerrit J. Schuurhuis, Arjan A. van de Loosdrecht
Summary: Myelodysplastic syndromes (MDS) are hematological disorders originating from neoplastic transformation of hematopoietic stem cells (HSCs). This study highlights the presence of immunophenotypic aberrant HSCs (IA-HSCs) in MDS-derived bone marrows (MDS-BMs), which predict leukemic progression and show potential as a prognostic biomarker. IA-HSCs are associated with perturbed hematopoiesis and independent of conventional risk factors. The sensitivity and specificity of IA-HSCs for predicting leukemic progression at 2 years follow-up were 83% and 71%, respectively.
Article
Cell & Tissue Engineering
Kenji Kitajima, Minako Shingai, Hikaru Ando, Mako Hamasaki, Takahiko Hara
Summary: Researchers developed a novel method for hematopoietic differentiation of human-induced pluripotent stem cells to address the issue of low production of CD38(+) hematopoietic progenitor cells. They found that the lack of FLT3 expression was responsible for this problem and solved it by adding interferon-gamma.
Article
Multidisciplinary Sciences
Junichiro Takano, Shinsuke Ito, Yixing Dong, Jafar Sharif, Yaeko Nakajima-Takagi, Taichi Umeyama, Yong-Woon Han, Kyoichi Isono, Takashi Kondo, Yusuke Iizuka, Tomohiro Miyai, Yoko Koseki, Mika Ikegaya, Mizuki Sakihara, Manabu Nakayama, Osamu Ohara, Yoshinori Hasegawa, Kosuke Hashimoto, Erik Arner, Robert J. Klose, Atsushi Iwama, Haruhiko Koseki, Tomokatsu Ikawa
Summary: Here the authors demonstrate that a variant PRC1 containing PCGF1 prevents excessive loading of transcriptional activators and chromatin remodelers on nascent DNA during DNA replication, thereby ensuring proper deposition of nucleosomes and downstream chromatin configurations in hematopoietic stem and progenitor cells (HSPCs). This finding highlights the importance of PCGF1-PRC1 in maintaining the differentiation potential of HSPCs and lifelong hematopoiesis.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
You Jung Hwang, Dong-Yeop Shin, Min-Jung Kim, Hyosun Jang, Soyeon Kim, Hyunwon Yang, Won Il Jang, Sunhoo Park, Sehwan Shim, Seung Bum Lee
Summary: This study found that SR1 could alleviate radiation-induced hematopoietic injury. It increased the survival rate and promoted the recovery of peripheral blood cell counts in irradiated mice. In vitro experiments also showed that SR1 increased the population of human hematopoietic stem/progenitor cells, reduced radiation-induced DNA damage and apoptosis. These results suggest that SR1 may be a potential radiomitigator for hematopoietic injury.
Article
Cell & Tissue Engineering
Eric W. Martin, Alessandra Rodriguez y Baena, Roman E. Reggiardo, Atesh K. Worthington, Connor S. Mattingly, Donna M. Poscablo, Jana Krietsch, Michael T. McManus, Susan Carpenter, Daniel H. Kim, E. Camilla Forsberg
Summary: Epigenetic mechanisms regulate the differentiation capacity of hematopoietic stem cells (HSCs). Open cis-regulatory elements (CREs) shared between HSCs and lineage cells remain accessible throughout differentiation. HSCs have greater chromatin accessibility and selective CREs play a functional role in lineage-specific gene expression.
Article
Hematology
Raghuveer Singh Mali, Qi Zhang, Rosa Anna DeFilippis, Antonio Cavazos, Vinitha Mary Kuruvilla, Jayant Raman, Vidhi Mody, Edna F. Choo, Monique Dail, Neil P. Shah, Marina Konopleva, Deepak Sampath, Elisabeth A. Lasater
Summary: The combination of FLT3-ITD inhibition with venetoclax demonstrates impressive anti-tumor activity in FLT3-ITD+ AML preclinical models, highlighting the strong mechanistic rationale for clinical studies.
Article
Pharmacology & Pharmacy
Sebastian Medina, Haikun Zhang, Laura V. Santos-Medina, Guanghua Wan, Alicia M. Bolt, Xixi Zhou, Scott W. Burchiel, Ke Jian Liu
Summary: Arsenic exposure disrupts hematopoiesis by inhibiting the function of GATA-2, leading to aberrant differentiation and cell accumulation, which may contribute to the onset and/or exacerbation of hematological disorders such as anemia.
TOXICOLOGY AND APPLIED PHARMACOLOGY
(2022)
Article
Multidisciplinary Sciences
Irene Mariam Roy, P. V. Anu, Samantha Zaunz, Srinu Reddi, Aravind M. Giri, Rithika Saroj Sankar, Sarah Schouteden, Joerg Huelsken, Catherine M. Verfaillie, Satish Khurana
Summary: Interaction with microenvironmental factors is crucial for the regulation of hematopoietic stem cell function. In this study, it was found that integrin signaling regulates the expression of SDF-1 alpha through transcriptional regulation. Inhibition of SRC gene or pharmacological inhibition resulted in increased expression of the chemokine SDF-1 alpha. Systemic inhibition of SRC led to increased SDF-1 alpha levels in BM plasma, which improved the homing efficiency of transplanted HSCs and enhanced hematopoietic function.
Article
Multidisciplinary Sciences
Yang Zhao, Chenxu Zhao, Han Guo, Zhaoqi Zhang, Huawen Xu, Mingpu Shi, Yanan Xu, Dong Wei, Yong Zhao
Summary: Rictor deletion leads to reduced production of granulocyte-monocyte progenitors (GMPs), monocytes, and macrophages, while neutrophil levels remain unaffected. Loss of Rictor inhibits monocyte differentiation while promoting neutrophil differentiation. The mTORC2 pathway regulates monocyte development through the AKT-Foxo4-ATF5-mtUPR pathway.
Article
Multidisciplinary Sciences
Ha Youn Shin, Chaochen Wang, Hye Kyung Lee, Kyung Hyun Yoo, Xianke Zeng, Tyler Kuhns, Chul Min Yang, Teresa Mohr, Chengyu Liu, Lothar Hennighausen
NATURE COMMUNICATIONS
(2017)
Article
Biochemistry & Molecular Biology
Nimitha R. Mathew, Francis Baumgartner, Lukas Braun, David O'Sullivan, Simone Thomas, Miguel Waterhouse, Tony A. Mueller, Kathrin Hanke, Sanaz Taromi, Petya Apostolova, Anna L. Illert, Wolfgang Melchinger, Sandra Duquesne, Annette Schmitt-Graeff, Lena Osswald, Kai-Li Yan, Arnim Weber, Sonia Tugues, Sabine Spath, Dietmar Pfeifer, Marie Follo, Rainer Claus, Michael Luebbert, Christoph Rummelt, Hartmut Bertz, Ralph Waesch, Johanna Haag, Andrea Schmidts, Michael Schultheiss, Dominik Bettinger, Robert Thimme, Evelyn Ullrich, Yakup Tanriver, Giang Lam Vuong, Renate Arnold, Philipp Hemmati, Dominik Wolf, Markus Ditschkowski, Cordula Jilg, Konrad Wilhelm, Christian Leiber, Sabine Gerull, Joerg Halter, Claudia Lengerke, Thomas Pabst, Thomas Schroeder, Guido Kobbe, Wolf Roesler, Soroush Doostkam, Stephan Meckel, Kathleen Stabla, Stephan K. Metzelder, Sebastian Halbach, Tilman Brummer, Zehan Hu, Joern Dengjel, Bjoern Hackanson, Christoph Schmid, Udo Holtick, Christof Scheid, Alexandros Spyridonidis, Friedrich Stoelzel, Rainer Ordemann, Lutz P. Mueller, Flore Sicre-de-Fontbrune, Gabriele Ihorst, Juergen Kuball, Jan E. Ehlert, Daniel Feger, Eva-Maria Wagner, Jean-Yves Cahn, Jacqueline Schnell, Florian Kuchenbauer, Donald Bunjes, Ronjon Chakraverty, Simon Richardson, Saar Gill, Nicolaus Kroeger, Francis Ayuk, Luca Vago, Fabio Ciceri, Antonia M. Mueller, Takeshi Kondo, Takanori Teshima, Susan Klaeger, Bernhard Kuster, Dennis (Dong Hwan) Kim, Daniel Weisdorf, Walter van der Velden, Daniela Doerfel, Wolfgang Bethge, Inken Hilgendorf, Andreas Hochhaus, Geoffroy Andrieux, Melanie Boerries, Hauke Busch, John Magenau, Pavan Reddy, Myriam Labopin, Joseph H. Antin, Andrea S. Henden, Geoffrey R. Hill, Glen A. Kennedy, Merav Bar, Anita Sarma, Donal McLornan, Ghulam Mufti, Betul Oran, Katayoun Rezvani, Omid Shah, Robert S. Negrin, Arnon Nagler, Marco Prinz, Andreas Burchert, Andreas Neubauer, Dietrich Beelen, Andreas Mackensen, Nikolas von Bubnoff, Wolfgang Herr, Burkhard Becher, Gerard Socie, Michael A. Caligiuri, Eliana Ruggiero, Chiara Bonini, Georg Haecker, Justus Duyster, Juergen Finke, Erika Pearce, Bruce R. Blazar, Robert Zeiser
Letter
Biochemical Research Methods
Michaela Willi, Harold E. Smith, Chaochen Wang, Chengyu Liu, Lothar Hennighausen
Letter
Oncology
Tony Andreas Mueller, Sandra Pennisi, Anabel Zwick, Sarah Decker, Claudius Klein, Benjamin Rister, Alina Rudorf, Sandra Kissel, Marie Follo, Ralph Waesch, Anna Lena Illert, Dietmar Pfeifer, Robert Oostendorp, Claudia Waskow, Christine Dierks, Justus Duyster
Article
Hematology
Alina Rudorf, Tony Andreas Mueller, Cathrin Klingeberg, Stefanie Kreutmair, Teresa Poggio, Sivahari Prasad Gorantla, Tamina Rueckert, Annette Schmitt-Graeff, Anina Gengenbacher, Peter Paschka, Claudia Baldus, Robert Zeiser, George S. Vassiliou, Allan Bradley, Justus Duyster, Anna Lena Illert
Article
Hematology
Chuanjiang Yu, Sivahari P. Gorantla, Alina Mueller-Rudorf, Tony A. Mueller, Stefanie Kreutmair, Corinna Albers, Lena Jakob, Lena J. Lippert, Zhenyu Yue, Monika Engelhardt, Marie Follo, Robert Zeiser, Tobias B. Huber, Justus Duyster, Anna L. Illert
Article
Biochemistry & Molecular Biology
Xianke Zeng, Hye Kyung Lee, Chaochen Wang, Precious Achikeh, Chengyu Liu, Lothar Hennighausen
EXPERIMENTAL AND MOLECULAR MEDICINE
(2020)
Article
Genetics & Heredity
Dana Lau-Corona, Woo Kyun Bae, Lothar Hennighausen, David J. Waxman
Article
Hematology
S. Oberbeck, A. Schrader, K. Warner, D. Jungherz, G. Crispatzu, J. von Jan, M. Chmielewski, A. Ianevski, H. H. Diebner, P. Mayer, A. Kondo Ados, L. Wahnschaffe, T. Braun, T. A. Mueller, P. Wagle, A. Bouska, T. Neumann, S. Puetzer, L. Varghese, N. Pflug, M. Thelen, J. Makalowski, N. Riet, H. J. M. Goex, G. Rappl, J. Altmueller, M. Kotrova, T. Persigehl, G. Hopfinger, M. L. Hansmann, H. Schloesser, S. Stilgenbauer, J. Duerig, D. Mougiakakos, M. Von Bergwelt-Baildon, I Roeder, S. Hartmann, M. Hallek, R. Moriggl, M. Brueggemann, T. Aittokallio, J. Iqbal, S. Newrzela, H. Abken, M. Herling
Article
Cell Biology
Lothar Hennighausen, Hye Kyung Lee
Article
Multidisciplinary Sciences
Hye Kyung Lee, Michaela Willi, Tyler Kuhns, Chengyu Liu, Lothar Hennighausen
Summary: Enhancers that activate gene expression in the lactating mouse mammary gland were investigated, revealing the importance of a lactation-specific distal enhancer and a super-enhancer in coordinating with the promoter for gene activation. The study provides insights into the regulatory mechanisms of enhancers and super-enhancers during lactation.
NATURE COMMUNICATIONS
(2021)
Article
Biology
Hye Kyung Lee, Olive Jung, Lothar Hennighausen
Summary: The study reveals that interferon-induced expression of dACE2 and ACE2 genes can be suppressed by JAK inhibitors, providing insights into SARS-CoV-2 infection of airway cells and potential therapeutic strategies.
COMMUNICATIONS BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Lena Jakob, Tony Andreas Mueller, Michael Rassner, Helen Kleinfelder, Pia Veratti, Jan Mitschke, Cornelius Miething, Robert A. J. Oostendorp, Dietmar Pfeifer, Miguel Waterhouse, Justus Duyster
Summary: The IL-6 family cytokine Oncostatin M (OSM) has various effects on cell development, growth, hematopoiesis, inflammation, and cancer. It can have both proliferative and antiproliferative effects depending on the target cell, with mechanisms involving OSM receptors and signaling pathways. Studies in murine cells show that OSM affects proliferation in stromal and fibroblast cell lines by activating JAK-STAT, PI3K-AKT, and MAPK-ERK pathways through OSMR, offering new insights into proliferation regulation by mOSM.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Hye Kyung Lee, Ludwig Knabl, Mary Walter, Priscilla A. Furth, Lothar Hennighausen
Summary: The study suggests that prior infection status significantly impacts the immune response following BA.2 infection, with significantly increased neutralizing antibody levels in patients with a previous history of COVID-19.
Article
Immunology
Alejandro V. Villarino, Arian D. J. Laurence, Fred P. Davisi, Luis Nivelo, Stephen R. Brooks, Hong-Wei Sun, Kan Jiang, Behdad Afzali, Daniela Frasca, Lothar Hennighausen, Yuka Kanno, John J. O'Shea
Summary: This study reveals that STAT5 is a crucial regulator of energy and amino acid metabolism in CD4+ T helper cells, and it plays a role in transcriptional regulation of key enzymes and transporters. Additionally, STAT5 interacts with the mTOR signaling pathway and MYC transcription factor to regulate T-cell metabolism.
SCIENCE IMMUNOLOGY
(2022)