期刊
LASERS IN MEDICAL SCIENCE
卷 31, 期 8, 页码 1613-1619出版社
SPRINGER LONDON LTD
DOI: 10.1007/s10103-016-2025-5
关键词
Diode laser; Dental implants; Titanium oxide; Staphylococcus aureus; Lipopolysaccharide
资金
- General Project Ltd. (Montespertoli, Florence, Italy)
Effective decontamination of biofilm and bacterial toxins from the surface of dental implants is a yet unresolved issue. This in vitro study aims at providing the experimental basis for possible use of diode laser (lambda 808 nm) in the treatment of peri-implantitis. Staphylococcus aureus biofilm was grown for 48 h on titanium discs with porous surface corresponding to the bone-implant interface and then irradiated with a diode laser (lambda 808 nm) in noncontact mode with airflow cooling for 1 min using a 600-mu m fiber. Setting parameters were 2 W (400 J/cm(2)) for continuous wave mode; 22 mu J, 20 kHz, 7 mu s (88 J/cm(2)) for pulsed wave mode. Bactericidal effect was evaluated using fluorescence microscopy and counting the residual colony-forming units. Biofilm and titanium surface morphology were analyzed by scanning electron microscopy (SEM). In parallel experiments, the titanium discs were coated with Escherichia coli lipopolysaccharide (LPS), laser-irradiated and seeded with RAW 264.7 macrophages to quantify LPS-driven inflammatory cell activation by measuring the enhanced generation of nitric oxide (NO). Diode laser irradiation in both continuous and pulsed modes induced a statistically significant reduction of viable bacteria and nitrite levels. These results indicate that in addition to its bactericidal effect laser irradiation can also inhibit LPS-induced macrophage activation and thus blunt the inflammatory response. The lambda 808-nm diode laser emerges as a valuable tool for decontamination/detoxification of the titanium implant surface and may be used in the treatment of peri-implantitis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据