Article
Biochemistry & Molecular Biology
Ryoma Tagawa, Masaki Kobayashi, Misako Sakurai, Maho Yoshida, Hiroki Kaneko, Yuhei Mizunoe, Yuka Nozaki, Naoyuki Okita, Yuka Sudo, Yoshikazu Higami
Summary: Long-term feeding of taurine lowers plasma cholesterol and bile acids by suppressing the FGF21/ERK pathway and upregulating Cyp7a1 expression, promoting cholesterol metabolism.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Pavlo Khodakivskyi, Christian L. Lauber, Aleksey Yevtodiyenko, Arkadiy A. Bazhin, Stephen Bruce, Tamar Ringel-Kulka, Yehuda Ringel, Bertrand Betrisey, Joana Torres, Jianzhong Hu, Chieh Jason Chou, Elena A. Goun
Summary: The study developed a novel optical tool for quantifying BSH activity across various biological contexts in a quick and cost-effective manner, with potential clinical applications, particularly in predicting the clinical status of IBD patients.
Article
Biochemistry & Molecular Biology
Tim Kroll, Sander H. J. Smits, Lutz Schmitt
Summary: ABCB4, also known as multidrug-resistant protein 3 (MDR3), is an ATP binding cassette transporter that specifically moves phosphatidylcholine (PC) lipids from the cytoplasmic to the extracellular leaflet in hepatocytes. Different bile acids can modulate its ATPase activity, with a nearly linear correlation between the critical micelle concentration and maximal stimulation by each bile acid. This study reveals the significant impact of 24-nor-ursodeoxycholic acid on ABCB4, suggesting a distinct regulation of ATPase activity compared to other bile acids.
JOURNAL OF LIPID RESEARCH
(2021)
Review
Nutrition & Dietetics
Frans Stellaard
Summary: Dietary cholesterol plays a significant role in the endogenous cholesterol pool and affects the serum concentration of total cholesterol, particularly LDL cholesterol. Maintaining a balance in hepatic cholesterol metabolism is crucial for preventing the risk of atherosclerosis and cardiovascular diseases.
Article
Fisheries
Alberto Ruiz, Karl B. Andree, Ignasi Sanahuja, Paul G. Holhorea, Josep A. Calduch-Giner, Sofia Morais, Jose J. Pastor, Jaume Perez-Sanchez, Enric Gisbert
Summary: The present study aimed to evaluate the effect of dietary supplementation using bile salts on growth and feed performance, fat accumulation and tissue condition in gilthead seabream. It was found that the supplementation of 0.06% bile salts in the diet significantly increased the fish's body weight and decreased the level of perivisceral fat. Furthermore, the bile acid profile in the gallbladder and anterior intestine was modified by the dietary supplementation of bile salts.
Article
Chemistry, Physical
Filipe Coreta-Gomes, Ines M. V. Silva, Claudia Nunes, Ildefonso Marin-Montesinos, Dmitry Evtuguin, Carlos F. G. C. Geraldes, Maria Joao Moreno, Manuel A. Coimbra
Summary: Chitooligosaccharides have been found to effectively bind bile salts and reduce cholesterol accessibility. While the ionic interaction between chitooligosaccharides and bile salts is often cited as the main mechanism, this study suggests that non-ionic interactions may also play a significant role. The non-ionic character of chitooligosaccharides is considered an important structural feature for the development of hypocholesterolemic ingredients.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Mihalj Posa
Summary: Bile acid anions are steroidal biosurfactants that form micelles through the hydrophobic effect. Monoketo derivatives of cholic acid are important for biopharmaceutical examinations due to their reduced membrane toxicity. The formation of primary and secondary micelles is separated, and the new mechanism of secondary micelle formation is explained through the analysis of spin-lattice relaxation time dependence on surfactant concentration. The association of 7-oxodeoxycholic acid anions is observed through two critical micellar concentrations and the aggregation number of the micelle changes abruptly within a specific concentration interval.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Food Science & Technology
Jimmy G. Hernandez-Gomez, Argelia Lopez-Bonilla, Gabriela Trejo-Tapia, Sandra V. Avila-Reyes, Antonio R. Jimenez-Aparicio, Humberto Hernandez-Sanchez
Summary: The study evaluated the BSH activity in six probiotic strains, including five lactic acid bacteria and one probiotic yeast. Results showed that Lactobacillus plantarum DGIA1 and Saccharomyces boulardii exhibited excellent deconjugation activities against bile acids, with the yeast showing BSH activity for the first time. This finding highlights the potential of these probiotic strains in regulating cholesterol levels.
Article
Food Science & Technology
Julieta N. Naso, Fernando A. Bellesi, Victor M. Pizones Ruiz-Henestrosa, Ana M. R. Pilosof
Summary: This study demonstrates that the solubilization capacity of bile salt micelles for fatty acids is influenced by emulsion components, as well as the concentration of the emulsifier. The increased concentration of the emulsifier affects the kinetics of lipolysis in oil-in-water emulsions, potentially impacting the bioavailability of fatty acids.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Biochemistry & Molecular Biology
Ai Takeyama, Asami Teramoto, Tianyu Wang, Takuya Hayashi, Yasutake Tanaka, Masao Sato, Bungo Shirouchi
Summary: This study found that dietary LysoPL affects lymphatic fat transport, especially in reducing cholesterol transport significantly. Compared to a phospholipid diet, both sn-1 and sn-2 LysoPL diets decreased the lymphatic transport of cholesterol, but had no effect on PL and TAG transport; in addition, LysoPL also affected the solubility of cholesterol in bile acid micelles.
Article
Chemistry, Physical
Sagar Kumar Behera, Srinibash Mishra, Monalisa Mohapatra
Summary: The interaction between cellulose ether and bile salt was investigated using various methods, revealing its potential impact on lipid digestion. By calculating different parameters, the micellization properties of the MC/NaDC mixed system were explored. This study provides a comprehensive understanding of the mixed system's implications on lipid digestion.
COLLOID AND INTERFACE SCIENCE COMMUNICATIONS
(2021)
Article
Biotechnology & Applied Microbiology
Saman Javed, Aneela Munir, Ghulam Ayesha Javed, Zakia Latif, Sehrish Javed, Najma Arshad
Summary: This study genetically characterized probiotics from human milk, demonstrating a link between genetic diversity and cholesterol reduction potential. It also confirmed the presence of conserved bsh genes in genetically diverse species. The strains showed cholesterol reduction ranging from 2.78% to 42.23%, independent of prebiotics.
LETTERS IN APPLIED MICROBIOLOGY
(2023)
Article
Chemistry, Physical
Sachin Rathod, Amita Joshi, Debes Ray, Vinod K. Aswal, Gunjan Verma, Pratap Bahadur, Sanjay Tiwari
Summary: The research found that the effect of NaC on TPGS aggregates depends on its concentration. At low concentrations, NaC molecules migrate to the core of micelles leading to contraction, while at higher concentrations, NaC molecules are driven away to the shell region causing swelling of the aggregates.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2021)
Article
Biochemistry & Molecular Biology
Xuebin Gao, Xiaozhuan Lin, Yan Xin, Xuan Zhu, Xiang Li, Ming Chen, Zhigang Huang, Honghui Guo
Summary: Nonalcoholic fatty liver disease (NAFLD) is a common chronic liver disorder globally. A study found that high dietary cholesterol intake accelerates the progression of NAFLD and induces liver inflammation in mice. Additionally, certain bacterial species in the gut microbiota were found to be positively correlated with the content of unconjugated bile acids in the liver. High dietary cholesterol alters gut microbiota composition and bile acid metabolism, promoting the development of NASH.
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2023)
Article
Gastroenterology & Hepatology
Arthur Kastl, Wenjing Zong, Victoria M. Gershuni, Elliot S. Friedman, Ceylan Tanes, Adoma Boateng, William J. Mitchell, Kathleen O'Connor, Kyle Bittinger, Natalie A. Terry, Christina Bales, Lindsey Albenberg, Gary D. Wu
Summary: This study investigates the effect of dietary fiber on patients with short bowel syndrome (SBS) and finds that SBS patients have lower diversity in their gut microbiota, which is inversely correlated with whole food consumption. Whole food intake is associated with increased glycoside hydrolases (GH) and bile salt hydrolases (BSH) and reduced fecal conjugated bile acids. Dietary fiber may regulate bile acid metabolism through the activity of gut microbiota enzymes, leading to intolerance of whole food in SBS patients.
Article
Medicine, General & Internal
Rune Matthiesen, Chris Lauber, Julio L. Sampaio, Neuza Domingues, Liliana Alves, Mathias J. Gerl, Manuel S. Almeida, Gustavo Rodrigues, Pedro Araujo Goncalves, Jorge Ferreira, Claudia Borbinha, Joao Pedro Marto, Marisa Neves, Frederico Batista, Miguel Viana-Baptista, Jose Alves, Kai Simons, Winchil L. C. Vaz, Otilia V. Vieira
Summary: The study demonstrates that chronic inflammatory diseases exhibit distinct plasma lipid profiles, which can be accurately distinguished using lipidomics to identify different diseases and subclasses, especially in cardiovascular diseases.
Article
Cell Biology
Ricardo C. Abreu, Cristiana Ramos, Clarissa Becher, Miguel Lino, Carlos Jesus, Paula A. da Costa Martins, Patricia A. T. Martins, Maria Joao Moreno, Hugo Fernandes, Lino Ferreira
Summary: The study demonstrates that Exo-Fect is the most efficient method for modulating sEVs, leading to over 1000-fold upregulation of the miRNA of interest and successful delivery of miR-155-5p into target cells, demonstrating its functionality. Furthermore, the membrane alterations in Exo-Fect-loaded sEVs enhance internalization within target cells and decrease interaction with lysosomes.
JOURNAL OF EXTRACELLULAR VESICLES
(2021)
Article
Biochemistry & Molecular Biology
Nisa Magalhaes, Guilherme M. Simoes, Cristiana Ramos, Jaime Samelo, Alexandre C. Oliveira, Hugo A. L. Filipe, Joao P. Prates Ramalho, Maria Joao Moreno, Luis M. S. Loura
Summary: This study combines atomistic molecular dynamics (MD) simulations and fluorescence spectroscopy to characterize the interaction between rhodamines 123 and B (Rh123 and RhB, respectively) and POPC bilayers. The study provides detailed insights on the similarities and differences in the behavior of bilayer-inserted Rh123 and RhB, related to the structure of the probes. The higher affinity of RhB for the membranes increases the local concentration and explains its higher apparent affinity for P-glycoprotein reconstituted in model membranes.
Article
Food Science & Technology
Maria Joao Primitivo, Marta Neves, Cristiana L. Pires, Pedro F. Cruz, Catarina Brito, Ana C. Rodrigues, Carla C. C. R. de Carvalho, Megan M. Mortimer, Maria Joao Moreno, Rui M. M. Brito, Edward J. Taylor, Stefan H. Millson, Fernando Reboredo, Maria Jorge Campos, Daniela C. Vaz, Vania S. Ribeiro
Summary: Helichrysum italicum flowers are rich in nutrients and bioactive compounds, making them suitable for use as food additives and natural preservatives. They exhibit antimicrobial and anti-inflammatory properties and are non-toxic to human intestinal cells.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Chemistry, Multidisciplinary
Cristiana Ramos, Jaime Samelo, Patricia A. T. Martins, Maria Joao Moreno
Summary: The amount of protein in food is an important characteristic, and there are several methods available for quantification. This study presents a method for protein quantification in food using precipitation and colorimetric quantification. The method is visually appealing and suitable for students of all ages. It also presents an interesting challenge for higher level students in interpreting the results.
JOURNAL OF CHEMICAL EDUCATION
(2022)
Article
Biochemistry & Molecular Biology
Margarida M. Cordeiro, Armindo Salvador, Maria Joao Moreno
Summary: Predicting the rate of substance permeation through membrane barriers is crucial for drug development. However, calculating permeability coefficients accurately is not always straightforward. In this study, a kinetic model for solute permeation through lipid membrane barriers is developed, and useful relationships are established to overcome limitations in calculations and predict permeability in specific in vitro or in vivo systems.
Article
Chemistry, Inorganic & Nuclear
Alexandre C. Oliveira, Hugo A. . L. Filipe, Joao P. Prates Ramalho, Armindo Salvador, Carlos F. G. C. Geraldes, Maria Joao Moreno, Luis M. S. Loura
Summary: The correct parametrization of lanthanide complexes is crucial for their characterization using computational tools, and this study establishes the best strategies for parametrization and demonstrates the successful capture of dynamic properties.
INORGANIC CHEMISTRY
(2022)
Review
Biochemistry & Molecular Biology
Maria Joao Moreno, Luis M. S. Loura, Jorge Martins, Armindo Salvador, Adrian Velazquez-Campoy
Summary: This review article discusses various methods and formalisms for analyzing the equilibrium distribution of ligands in the presence of proteins, lipid membranes, or both, with special attention to common pitfalls and validity limits of different approaches. The analysis of ligand binding through Stern-Volmer plots of protein fluorescence quenching is emphasized for its widespread use in characterizing interactions. Different systems of increasing complexity are considered, from proteins with single to multiple binding sites to membrane proteins in lipid bilayers.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Food Science & Technology
Cristiana L. Pires, Ines M. Silva, Manuel A. Coimbra, Maria Joao Moreno, Filipe Coreta-Gomes
Summary: This study evaluated the effects of coffee with different commercial roasting and grinding levels on cholesterol absorption. It was found that coffee extracts can decrease the permeability coefficient of cholesterol and affect its bioavailability through increased cholesterol precipitation. Dark roasting and finer grinding of coffee showed stronger hypocholesterolemic effects.
Article
Pharmacology & Pharmacy
Maria Joao Moreno, Hugo A. L. Filipe, Susana V. P. Cunha, Cristiana V. Ramos, Patricia A. T. Martins, Biebele Abel, Luis M. S. Loura, Suresh V. Ambudkar
Summary: The transport of drugs by efflux transporters plays a crucial role in drug resistance development by cancer cells and pathogens. In this study, the interaction between a series of fluorescent amphiphiles and the efflux transporter P-glycoprotein (P-gp) was explored. The results showed that the binding parameters obtained were apparent and a new formalism was proposed to better understand the binding affinity of amphiphiles with P-gp. It was found that the polar interactions are essential for this interaction, and an increase in lipophilicity and amphiphilicity enhanced the association with the lipid bilayer, inhibiting the function of P-gp.
Article
Chemistry, Medicinal
Ana S. P. Moreira, Diana Gaspar, Sonia S. Ferreira, Alexandra Correia, Manuel Vilanova, Marie-Mathilde Perrineau, Philip D. Kerrison, Claire M. M. Gachon, Maria Rosario Domingues, Manuel A. Coimbra, Filipe M. Coreta-Gomes, Claudia Nunes
Summary: This study aimed to characterize the chemical structure of water-soluble polysaccharides from Saccharina latissima and evaluate their immunostimulatory and hypocholesterolemic activities. It was found that laminarans and two fractions of fucose-containing sulphated polysaccharides showed immunostimulatory activity on B lymphocytes, and one of the fractions exhibited a significant effect in reducing cholesterol bioaccessibility.
Article
Biochemistry & Molecular Biology
Maria Joao Moreno, Armindo Salvador
Summary: Ligand-protein interactions in complex media with lipids can influence the apparent affinity and should be considered when studying protein interactions. This study presents a framework to quantitatively characterize these interactions and explores the validity of commonly used approximations. The results show that ligand lipophilicity and lipid bilayer volume impact the apparent ligand-protein affinity, with exceptions for very polar ligands and ligands with supralinear increase in affinity with lipophilicity. The study also discusses important parameters for exploring the specificity of membrane proteins.
Article
Biochemistry & Molecular Biology
Hugo A. L. Filipe, Luis M. S. Loura, Maria Joao Moreno
Summary: Permeation through biomembranes is crucial for drugs to reach their targets, and the asymmetry of cell plasma membrane plays an important role in this process. This study investigates the interaction of a series of NBD-labeled amphiphiles with lipid bilayers of different compositions, including an asymmetric bilayer. The behavior of the amphiphiles during permeation, including their orientation, chain elongation, and H-bonding, is described. The permeability coefficients of the amphiphiles are also calculated, but quantitative agreement with experimental values could not be achieved.
Article
Chemistry, Physical
Filipe Coreta-Gomes, Ines M. V. Silva, Claudia Nunes, Ildefonso Marin-Montesinos, Dmitry Evtuguin, Carlos F. G. C. Geraldes, Maria Joao Moreno, Manuel A. Coimbra
Summary: Chitooligosaccharides have been found to effectively bind bile salts and reduce cholesterol accessibility. While the ionic interaction between chitooligosaccharides and bile salts is often cited as the main mechanism, this study suggests that non-ionic interactions may also play a significant role. The non-ionic character of chitooligosaccharides is considered an important structural feature for the development of hypocholesterolemic ingredients.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2023)