Article
Biotechnology & Applied Microbiology
Pat M. Fidopiastis, Chaz Childs, Jeremy J. Esin, Jordan Stellern, Anna Darin, Andrea Lorenzo, Vanessa T. Mariscal, Jason Lorenz, Vinay Gopan, Sarah McAnulty, Karen L. Visick
Summary: Bacteria utilize extracellular DNA as a nutritious resource, and the nuclease activity of V. fischeri plays a crucial role in colonization and mutualistic association with Euprymna scolopes. Understanding the specific role of nuclease activity in this process expands our knowledge of beneficial microbial associations.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Microbiology
Courtney N. Dial, Steven J. Eichinger, Randi Foxall, Christopher J. Corcoran, Alice H. Tischler, Robert M. Bolz, Cheryl A. Whistler, Karen L. Visick
Summary: Bacterial motility is crucial for the symbiotic colonization of the squid Euprymna scolopes by Vibrio fischeri, with strain KB2B1 exhibiting a unique migration pattern compared to ES114, possibly due to mutations in the quorum sensing pathway and DGC gene. The mutations shifted KB2B1 to a perceived low cell density state and inhibited migration, highlighting differences in regulatory control between strains.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Joshua J. Cohen, Steven J. Eichinger, Danae A. Witte, Connor J. Cook, Pat M. Fidopiastis, Jovanka Tepavcevic, Karen L. Visick
Summary: This study investigates the importance of competence factors in Vibrio fischeri, revealing potential factors that control its transformation process and deepening the understanding of competence mechanisms. By advancing the genetic manipulation of V. fischeri, this research contributes to the further exploration of genes involved in relevant physiological processes.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Pat M. Fidopiastis, Vanessa Mariscal, Jeanne-Marie McPherson, Sarah McAnulty, Anne Dunn, Eric Stabb, Karen L. Visick
Summary: The study reveals the crucial role of V. fischeri AmiB in beneficial colonization of squid, with its mutation affecting cellulosic biofilm production and triggering biological processes related to bacterial colonization and antibiotic susceptibility.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Microbiology
Clotilde Bongrand, Eric Koch, Daniel Mende, Anna Romano, Susannah Lawhorn, Margaret McFall-Ngai, Edward F. DeLong, Edward G. Ruby
Summary: Planktonic cells of the luminous marine bacterium Vibrio fischeri establish symbiotic relationship with newly hatched Euprymna scolopes bobtail squid by colonizing the light-emitting organ. The symbiont population can undergo significant genetic diversification, including horizontal gene transfer, over the course of their growth in the organ, providing a genetic mix for selection and transmission to the next host.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biology
Ericka D. Surrett, Kirsten R. Guckes, Shyan Cousins, Terry B. Ruskoski, Andrew G. Cecere, Denise A. Ludvik, C. Denise Okafor, Mark J. Mandel, Tim Miyashiro
Summary: To colonize a host, bacteria rely on signaling systems to convert information about the host environment into cellular activities. This study reveals how the bacterial symbiont Vibrio fischeri initially colonizes the light organ of the Hawaiian bobtail squid. It shows that the regulatory component Qrr1 is inhibited by the sensor kinase BinK, while its expression depends on s(54), LuxO, and SypG, ensuring its role in colonization. This regulatory mechanism is widespread in the Vibrionaceae family.
Article
Microbiology
Andrea M. M. Suria, Stephanie Smith, Lauren Speare, Yuzhou Chen, Iris Chien, Emily Grace Clark, Madelyn Krueger, Alexander M. Warwick, Hannah Wilkins, Alecia N. Septer
Summary: The widespread distribution of diverse T6SS in bacterial species and habitats plays a crucial role in interbacterial competition and interactions with eukaryotic cells. The study reveals that bacterial symbionts encode diverse T6SSs with homology to those from distantly related species, suggesting different impacts on host health. Understanding how T6SS promotes bacterial population propagation and impacts host health is essential.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Cell Biology
Katrina A. A. Gundlach, Janna Nawroth, Eva Kanso, Farzana Nasrin, Edward G. G. Ruby, Margaret McFall-Ngai
Summary: The Hawaiian bobtail squid rapidly responds to bacteria in the surrounding seawater after hatching, increasing the efficiency of Vibrio fischeri colonization in host tissues.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Microbiology
Silvia Moriano-Gutierrez, Edward G. Ruby, Margaret J. McFall-Ngai
Summary: The study reveals that the initial 24 hours of the squid-vibrio association trigger differential expression of host miRNAs, which target mRNAs associated with tissue remodeling and immune suppression. This indicates miRNAs play a key role in orchestrating transcriptional responses during the establishment of symbiosis, with potential evolutionary conservation across various symbiotic associations.
Article
Microbiology
Lauren Speare, Madison Woo, Katherine M. Bultman, Mark J. Mandel, Michael S. Wollenberg, Alecia N. Septer
Summary: Bacteria employ diverse competitive strategies to enhance fitness and promote their own propagation. Little is known about how symbiotic bacteria modulate competitive mechanisms as they compete for a host niche. Vibrio fischeri, a symbiotic bacterium with marine animals, utilizes a type VI secretion system (T6SS) for interbacterial competition during colonization of the Euprymna scolopes squid light organ. Different strains of V. fischeri exhibit varying response to host-specific cues in the competition process, suggesting evolution of competitive mechanisms in filling distinct niches.
Article
Microbiology
Clotilde Bongrand, Jamie S. Foster
Summary: This study examined the effects of simulated microgravity on the interactions between beneficial bacteria and their hosts. The results indicated that environmental stress, such as simulated microgravity, can alter the initiation of beneficial interactions between microbes and animals. The population levels of certain bacterial strains were lower in animals exposed to simulated microgravity, and there were shifts in the relative abundance of strains in co-colonised animals.
ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Microbiology
Ruth Y. Isenberg, David G. Christensen, Karen L. Visick, Mark J. Mandel
Summary: There is significant interest in studying the role of cyclic diguanylate (c-di-GMP) in bacteria, specifically its effect on biofilm formation and motility. However, less attention has been given to understanding its contribution to beneficial colonization. This study examines the role of c-di-GMP in the colonization of Vibrio fischeri bacteria in the Hawaiian bobtail squid. The results show that high levels of c-di-GMP severely impair colonization, while low levels have no effect. The study also highlights the importance of proper c-di-GMP regulation during colonization and identifies an interaction between different biofilm polysaccharides regulated by c-di-GMP in the host.
Article
Infectious Diseases
Yoowon Kwon, Young-Sun Cho, Yoo-Mi Lee, Seok-jin Kim, Jaewoong Bae, Su-Jin Jeong
Summary: This study evaluated the gut microbiome state in infants following systemic antibiotic treatment. The results showed that the composition of the gut microbiome in the antibiotic treatment group was altered, with increased relative abundance of Escherichia/Shigella and Bifidobacterium, and decreased relative abundance of Bacteroides. Additionally, the antibiotic treatment group had lower microbiome diversity. Long-term studies are needed to further understand the impact of antibiotics on the gut microbiome.
Article
Microbiology
Caleb-Matthew Olaso, Joani Viliunas, Margaret McFall-Ngai
Summary: Through studying the binary association between Hawaiian bobtail squid and Vibrio fischeri, a receptor protein EsPGRP4 has been identified as the mediator of host mucus secretion by sensing environmental PGN. These findings provide evidence of an innate mechanism that underlies a crucial first step in the horizontal recruitment of bacterial symbionts.
Article
Microbiology
Sarah J. McAnulty, Allison H. Kerwin, Eric Koch, Barrett Nuttall, Andrea M. Suria, Andrew J. Collins, Tyler R. Schleicher, Bethany A. Rader, Spencer V. Nyholm
Summary: Microbiota have significant impacts on animal and plant development. The absence of symbionts often leads to developmental defects or reduced organ function. In this study, it is shown that environmental bacteria are associated with the development of a symbiotic organ, the accessory nidamental gland (ANG), in female Hawaiian bobtail squid. The presence of certain bacterial groups, particularly Verrucomicrobia, is linked to ANG development.
Article
Biotechnology & Applied Microbiology
K. Nikolakakis, K. Monfils, S. Moriano-Gutierrez, C. A. Brennan, E. G. Ruby
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2016)
Article
Ecology
Clotilde Bongrand, Eric J. Koch, Silvia Moriano-Gutierrez, Otto X. Cordero, Margaret McFall-Ngai, Martin F. Polz, Edward G. Ruby
Article
Cell & Tissue Engineering
Javier Megias, Alberto Yanez, Silvia Moriano, Jose-Enrique O'Connor, Daniel Gozalbo, Maria-Luisa Gil
Article
Multidisciplinary Sciences
Mahdi Belcaid, Giorgio Casaburi, Sarah J. McAnulty, Hannah Schmidbaur, Andrea M. Suria, Silvia Moriano-Gutierrez, M. Sabrina Pankey, Todd H. Oakley, Natacha Kremer, Eric J. Koch, Andrew J. Collins, Hoan Nguyen, Sai Lek, Irina Goncharenko-Foster, Patrick Minx, Erica Sodergren, George Weinstock, Daniel S. Rokhsar, Margaret McFall-Ngai, Oleg Simakov, Jamie S. Foster, Spencer V. Nyholm
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Article
Medicine, General & Internal
Maria Jose Gosalbes, Joan Compte, Silvia Moriano-Gutierrez, Yvonne Valles, Nuria Jimenez-Hernandez, Xavier Pons, Alejandro Artacho, M. Pilar Francino
Article
Multidisciplinary Sciences
Silvia Moriano-Gutierrez, Eric J. Koch, Haley Bussan, Kymberleigh Romano, Mandi Belcaid, Federico E. Rey, Edward G. Ruby, Margaret J. Mcfall-Ngai
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Article
Microbiology
Clotilde Bongrand, Silvia Moriano-Gutierrez, Philip Arevalo, Margaret McFall-Ngai, Karen L. Visick, Martin Polz, Edward G. Ruby
Article
Biology
Eric J. Koch, Silvia Moriano-Gutierrez, Edward G. Ruby, Margaret McFall-Ngai, Manuel Liebeke
JOURNAL OF EXPERIMENTAL BIOLOGY
(2020)
Article
Multidisciplinary Sciences
Eric J. Koch, Clotilde Bongrand, Brittany D. Bennett, Susannah Lawhorn, Silvia Moriano-Gutierrez, Marko Pende, Karim Vadiwala, Hans-Ulrich Dodt, Florian Raible, William Goldman, Edward G. Ruby, Margaret McFall-Ngai
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Microbiology
Silvia Moriano-Gutierrez, Edward G. Ruby, Margaret J. McFall-Ngai
Summary: The study reveals that the initial 24 hours of the squid-vibrio association trigger differential expression of host miRNAs, which target mRNAs associated with tissue remodeling and immune suppression. This indicates miRNAs play a key role in orchestrating transcriptional responses during the establishment of symbiosis, with potential evolutionary conservation across various symbiotic associations.
Article
Microbiology
T. Essock-Burns, B. D. Bennett, D. Arencibia, S. Moriano-Gutierrez, M. Medeiros, M. J. McFall-Ngai, E. G. Ruby
Summary: This study demonstrates that quorum-sensing regulation by the Vibrio fischeri population induces a tissue phenotype that promotes the retention of this extracellular symbiont within the light organ of its host. Actin polymerization is identified as the primary mechanism underlying constriction, and host responses to the presence of symbionts change as a function of tissue maturation.
Review
Biochemistry & Molecular Biology
Amelie Cabirol, Silvia Moriano-Gutierrez, Philipp Engel
Summary: The gut microbiota of honey bees affects their brain and behavioral phenotypes, and the abundance of neuroactive metabolites in the gut, hemolymph, and brain is altered by the presence of microbiota.
MOLECULAR MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Silvia Moriano-Gutierrez, Clotilde Bongrand, Tara Essock-Burns, Leo Wu, Margaret J. McFall-Ngai, Edward G. Ruby