Article
Biochemistry & Molecular Biology
Boris K. Semin, Lira N. Davletshina
Summary: Ca-depleted photosystem II membranes obtained by treatment with acidic buffer lack Ca2+ in the Mn4CaO5 cluster, but contain all extrinsic proteins protecting this cluster (PSII(-Ca/low pH)). Unlike native photosystem II, the Mn cluster in PSII(-Ca/low pH) samples can be reduced by small-sized reductants. By substituting Mn cation(s) with Fe cation(s) at pH 6.5, a chimeric cluster (3Mn1Fe) was obtained in PSII(-Ca/low pH) membranes containing extrinsic proteins. The resulting membranes exhibited high intensity O-2 evolution in the presence of exogenous Ca2+, reaching about 80% of the rate in PSII(-Ca/low pH) membranes.
JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Wei Zhao, Xiao-Qi Yang, Quan-Sheng Zhang, Ying Tan, Zhe Liu, Ming-Yu Ma, Meng-Xin Wang, Bin Xu
Summary: Zostera marina, a widespread seagrass, partially inactivated its oxygen-evolving complex in response to light exposure, but still maintained a normal carbon assimilation level. Through an in vivo study, it was found that the photosynthetic regulation of this seagrass can be verified at both proteome and physiology levels.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
(2021)
Review
Plant Sciences
Charles F. Yocum
Summary: These special issues of photosynthesis research present papers documenting progress in revealing the many aspects of photosystem 2, a unique and complex system. The author provides a personal assessment of the progress observed over a four-decade research career, discussing some remaining unsolved issues.
PHOTOSYNTHESIS RESEARCH
(2022)
Article
Plant Sciences
Leidy Alves dos Santos, Bruno Lemos Batista, Allan Klynger da Silva Lobato
Summary: This study found that foliar application of 24-epibrassinolide (EBR) under high magnesium concentration can alleviate the negative effects of magnesium stress on soybean photosynthesis. EBR protects against oxidative stress, delays chlorophyll degradation, and improves magnesium content and photochemical efficiency.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Article
Cell Biology
Sergey K. Zharmukhamedov, Mehriban S. Shabanova, Margarita Rodionova, Irada M. Huseynova, Mehmet Sayim Karacan, Nurcan Karacan, Kubra Begum Asik, Vladimir D. Kreslavski, Saleh Alwasel, Suleyman Allakhverdiev
Summary: The effects of the [CuL2]Br-2 complex on PSII activity and chlorophyll fluorescence yield in spinach were investigated. The complex was found to inhibit photosynthetic oxygen evolution and the photoreduction of the primary quinone electron acceptor. The inhibition depended on the concentration of [CuL2]Br-2 and was not affected by artificial electron donors.
Article
Biochemistry & Molecular Biology
Divya Kaur, Yingying Zhang, Krystle M. Reiss, Manoj Mandal, Gary W. Brudvig, Victor S. Batista, M. R. Gunner
Summary: Photosystem II uses water as the primary electron source for photosynthesis, with water-filled channels playing a crucial role in bringing in and transporting water molecules. Analysis shows that the water chain in the broad channel is better connected, facilitating proton translocation. However, the specific roles of each pathway in the oxidation and reduction processes still need further investigation.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2021)
Article
Biochemistry & Molecular Biology
Brandon P. Russell, David J. Vinyard
Summary: The Mn4CaO5 oxygen-evolving complex in Photosystem II is crucial for water oxidation. D1 residue R334 participates in proton release and interacts with PsbO. A D1-R334G mutant destabilizes the OEC but stabilizes the S2 intermediate.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2024)
Review
Plant Sciences
Yang Chen, Boran Xu, Ruoqing Yao, Changhui Chen, Chunxi Zhang
Summary: The oxygen-evolving center in photosystem II plays a crucial role in the water splitting reaction of artificial photosynthesis. However, the lack of a precise chemical model has hindered the understanding of its structure-function relationship. Recent advances in synthesizing artificial clusters that closely mimic the oxygen-evolving center have provided an opportunity to study its structure-function relationship and design efficient catalysts for water splitting in artificial photosynthesis.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Multidisciplinary Sciences
Katherine H. Richardson, John J. Wright, Mantas Simenas, Jacqueline Thiemann, Ana M. Esteves, Gemma McGuire, William K. Myers, John J. L. Morton, Michael Hippler, Marc M. Nowaczyk, Guy T. Hanke, Maxie M. Roessler
Summary: Photosynthetic Complex I (PS-CI) links the oxidation of ferredoxin and reduction of plastoquinone to proton pumping across thylakoid membranes, generating ATP in photosynthesis. By overcoming challenges, the authors determined the reduction potentials of PS-CI's iron-sulfur clusters, providing insight into the bioenergetics of the electron transfer relay.
NATURE COMMUNICATIONS
(2021)
Article
Plant Sciences
Brandon P. Russell, David J. Vinyard
Summary: The study found that the first Mn2+ oxidation event in the assembly of the oxygen-evolving complex in Photosystem II requires chloride-facilitated deprotonation. This process is dependent on the presence of calcium at physiologically relevant pH values.
PHOTOSYNTHESIS RESEARCH
(2022)
Review
Plant Sciences
Christine H. Foyer, Guy Hanke
Summary: ROS like singlet oxygen, superoxide, and hydrogen peroxide serve as markers of living cells and are produced abundantly during oxygenic photosynthesis. These ROS act as signals within chloroplasts, influencing cellular processes and redox signaling pathways. The challenge lies in understanding the organized delivery of regulated ROS from the photosynthetic electron transport chain to transmit redox signals from the environment to the nucleus, with stromal carbohydrate metabolism also playing a key role in chloroplast signaling pathways.
Article
Biochemistry & Molecular Biology
Miho Ohnishi, Riu Furutani, Takayuki Sohtome, Takeshi Suzuki, Shinya Wada, Soma Tanaka, Kentaro Ifuku, Daisei Ueno, Chikahiro Miyake
Summary: The study found that deficiencies of different minerals resulted in distinct changes in PSI and PSII parameters in sunflower leaves, such as an increase or decrease in oxidized P700. Specific responses of parameters to each mineral deficiency allowed for diagnosis of the deficiency and identification of which mineral affected photosynthesis.
Article
Plant Sciences
Hiroyuki Mino
Summary: The temperature dependence of the formation of the g similar to 5 S-2 state electron paramagnetic resonance (EPR) signal in photosystem II (PSII) was investigated. It was found that the g similar to 5 signal can be produced at an illumination above 200 K, and its formation is half inhibited at approximately 215 K. The study also estimated the activation energy, enthalpy, and entropy of the g similar to 5 state formation.
PHOTOSYNTHESIS RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Hiroshi Kuroda, Keisuke Kawashima, Kazuyo Ueda, Takuya Ikeda, Keisuke Saito, Ryo Ninomiya, Chisato Hida, Yuichiro Takahashi, Hiroshi Ishikita
Summary: The study utilized MD simulations and mutagenesis to identify the proton-transfer pathway in photosystem II, with D1-Asp61 being crucial in the process.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2021)
Article
Plant Sciences
Andre Vidal-Meireles, Soujanya Kuntam, Eszter Szeles, David Toth, Juliane Neupert, Ralph Bock, Szilvia Z. Toth
Summary: The lifetime of PSBO in green algae is strongly influenced by light intensity and carbon availability. It is crucial for the stability of photosystem II and loss of PSBO leads to significant changes in cell morphology and cell cycle.
PLANT CELL AND ENVIRONMENT
(2023)
Article
Biology
Koji Kato, Tasuku Hamaguchi, Ryo Nagao, Keisuke Kawakami, Yoshifumi Ueno, Takehiro Suzuki, Hiroko Uchida, Akio Murakami, Yoshiki Nakajima, Makio Yokono, Seiji Akimoto, Naoshi Dohmae, Koji Yonekura, Jian-Ren Shen
Summary: By analyzing the structure of a PSI trimer from the ancient cyanobacterium Gloeobacter violaceus PCC 7421, we discovered two low-energy chlorophylls that are missing in this organism, shedding light on the evolutionary changes of low-energy chlorophylls in oxygenic photosynthetic organisms.
Article
Chemistry, Multidisciplinary
Xiaohong Liu, Pengcheng Liu, Hongjie Li, Zhen Xu, Lu Jia, Yan Xia, Minling Yu, Wenqin Tang, Xiaolei Zhu, Chao Chen, Yuanlin Zhang, Eriko Nango, Rie Tanaka, Fangjia Luo, Koji Kato, Yoshiki Nakajima, Shunpei Kishi, Huaxin Yu, Naoki Matsubara, Shigeki Owada, Kensuke Tono, So Iwata, Long-Jiang Yu, Jian-Ren Shen, Jiangyun Wang
Summary: One of the key objectives in chemistry research is to observe atomic motion during reactions in real time. A team of researchers has developed a method to genetically encode a xanthone amino acid into a protein, allowing for high selective and quantum efficient photo-induced C-H bond transformation, and capturing the elusive excited-state intermediates responsible for precise C-H bond activation.
Article
Plant Sciences
Kohei Kagatani, Ryo Nagao, Jian-Ren Shen, Yumiko Yamano, Shinichi Takaichi, Seiji Akimoto
Summary: This article investigates the functions and excitation relaxation dynamics of diadinoxanthin and diatoxanthin in photosynthesis. The study finds that the absorption spectra shift to longer wavelengths and the lifetimes of the excited states decrease after converting diadinoxanthin to diatoxanthin.
PHOTOSYNTHESIS RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Yoshiki Nakajima, Natsumi Ugai-Amo, Naoki Tone, Akiko Nakagawa, Masako Iwai, Masahiko Ikeuchi, Miwa Sugiura, Michihiro Suga, Shen Jian-Ren
Summary: The three psbA genes (psbA1, psbA2, and psbA3) in the thermophilic cyanobacterium Thermosynechococcus elongatus have different expression patterns in response to changes in the growth environment. We crystallized PSII dimers from strains expressing only one psbA gene (psbA2 or psbA3) and analyzed their structures. Our results showed functional differences in the D1 protein expressed from these psbA genes, providing a structural basis for further functional examination.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Chemistry, Physical
J. Langley, R. Purchase, S. Viola, A. Fantuzzi, G. A. Davis, Jian-Ren Shen, A. W. Rutherford, E. Krausz, N. Cox
Summary: Low-temperature, metastable electrochromism has been used to identify pigments in Photosystem I (PS I) from Thermosynechococcus vulcanus and Chroococcidiopsis thermalis. A minimum of seven pigments is required to accurately model the electrochromism of PS I. This study proposes a short list of candidates for the controversial chlorophyll f pigment in FRL C. thermalis that absorbs at 756 nm, and discusses the pros and cons of these assignments. Further experiments are suggested to better understand the functioning of FRL C. thermalis.
JOURNAL OF CHEMICAL PHYSICS
(2022)
Article
Biochemistry & Molecular Biology
Jing-Hua Chen, Weiwei Wang, Chen Wang, Tingyun Kuang, Jian-Ren Shen, Xing Zhang
Summary: This study reports the structure of the photosynthetic reaction center complex (RCC) in green sulfur bacteria, which consists of a RC core, two FMO trimers, and two new subunits. The structure reveals a new bacteriochlorophyll and provides insights into the energy transfer network within this photosynthetic apparatus.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Melinda Magyar, Gabor Sipka, Wenhui Han, Xingyue Li, Guangye Han, Jian-Ren Shen, Petar H. H. Lambrev, Gyozo Garab
Summary: By measuring the chlorophyll-a fluorescence transients produced by single-turnover saturating flashes, researchers identified the rate-limiting steps in the transition from dark to light of Photosystem II. They found that in samples treated with diuron, the first flash fully reduced the Q(A) quinone acceptor molecule but only generated an F-1 fluorescence transient.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Instruments & Instrumentation
Artoni Kevin R. Ang, Yasufumi Umena, Ayana Sato-Tomita, Naoya Shibayama, Naohisa Happo, Riho Marumi, Yuta Yamamoto, Koji Kimura, Naomi Kawamura, Yu Takano, Tomohiro Matsushita, Yuji C. Sasaki, Jian-Ren Shen, Kouichi Hayashi
Summary: X-ray fluorescence holography (XFH) is a powerful atomic resolution technique that directly images the local atomic structure of a target element in a material. The development of serial X-ray fluorescence holography allows for direct recording of hologram patterns before radiation damage occurs. This technique paves the way for future experiments on protein crystals to study the local atomic structures of their metal clusters.
JOURNAL OF SYNCHROTRON RADIATION
(2023)
Article
Multidisciplinary Sciences
Dan-Hong Li, Wenda Wang, Cuicui Zhou, Yan Zhang, Songhao Zhao, Yi-Ming Zhou, Rong-Yao Gao, Hai-Dan Yao, Li-Min Fu, Peng Wang, Jian-Ren Shen, Tingyun Kuang, Jian-Ping Zhang
Summary: The light-harvesting complex II (LHCII) of Bryopsis corticulans differs in chlorophyll and carotenoid compositions from that of spinach. Using time-resolved absorption spectroscopy, we investigated the ultrafast excitation dynamics of B-LHCII. Our results showed rapid photogeneration of Chl-Chl charge transfer (CT) species and revealed the transfer of singlet excitation between different chlorophyll and carotenoid molecules. The induction of Chl-Chl CT species by red-light excitation is a common occurrence for LHCIIs in light-harvesting form, as observed in S-LHCII.
Article
Biochemistry & Molecular Biology
Shota Shimizu, Haruya Ogawa, Naoki Tsuboshita, Takehiro Suzuki, Koji Kato, Yoshiki Nakajima, Naoshi Dohmae, Jian-Ren Shen, Ryo Nagao
Summary: In this study, we successfully purified PSI-monomer-PBS and PSI-dimer-PBS supercomplexes from iron-deficient cyanobacterium. This suggests that the binding between CpcL and PSI becomes tighter under iron deficiency, resulting in the formation of these two supercomplexes.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2023)
Article
Plant Sciences
Ryo Nagao, Yoshifumi Ueno, Miyu Furutani, Koji Kato, Jian-Ren Shen, Seiji Akimoto
Summary: LHCs play a crucial role in capturing light energy and transferring it to photosystem cores in oxygenic photosynthetic organisms. In this study, three types of preparations from the red alga Cyanidium caldarium were purified and examined. The results indicate that the expression, function, and structure of red algal PSI-LHCI supercomplexes are affected by the binding of LHCIs to PSI.
PHOTOSYNTHESIS RESEARCH
(2023)
Article
Plant Sciences
Ryo Nagao, Haruya Ogawa, Naoki Tsuboshita, Koji Kato, Reona Toyofuku, Tatsuya Tomo, Jian-Ren Shen
Summary: This study successfully isolated and characterized PSI monomers containing chlorophyll d and compared their properties with PSI trimers from A. marina. The polypeptide composition of the monomer was consistent with that of the trimer, but the absorption and fluorescence emission spectra were different, suggesting different formations of low-energy chlorophylls d between the two types of PSI cores. Based on these findings, the location of low-energy chlorophyll d in A. marina PSIs is discussed.
PHOTOSYNTHESIS RESEARCH
(2023)
Article
Multidisciplinary Sciences
Ryo Nagao, Koji Kato, Tasuku Hamaguchi, Yoshifumi Ueno, Naoki Tsuboshita, Shota Shimizu, Miyu Furutani, Shigeki Ehira, Yoshiki Nakajima, Keisuke Kawakami, Takehiro Suzuki, Naoshi Dohmae, Seiji Akimoto, Koji Yonekura, Jian-Ren Shen
Summary: By using cryo-EM, the authors determined the structure of a PSI monomer associated with six IsiA proteins in Anabaena, providing insights into the diversity and functions of IsiAs. IsiAs are expressed in cyanobacteria under iron-deficient conditions, and the binding properties and functional roles of IsiAs in PSI were still unknown in Anabaena. The cryo-EM structure of a PSI-IsiA supercomplex isolated from iron-deficient Anabaena revealed the association of six IsiA subunits with a PSI core monomer, and the absence of a PsaL subunit due to the occupation of its position by the C-terminal domain of IsiA2.
NATURE COMMUNICATIONS
(2023)
Article
Plant Sciences
Parveen Akhtar, Fanny Balog-Vig, Wenhui Han, Xingyue Li, Guangye Han, Jian-Ren Shen, Petar H. Lambrev
Summary: The spatial separation of photosystems I and II (PSI and PSII) is crucial for maintaining an efficient flow of excitation energy in photosynthesis. Unlike plant chloroplasts, cyanobacterial thylakoids do not form tightly appressed grana stacks, allowing energy spillover from PSII to PSI. Studies have shown that this spillover can reach a quantum yield of up to 40% in certain systems. These findings support the model of coexistence of laterally separated microdomains of PSI and PSII in cyanobacterial cells, as well as energetically connected domains between the two photosystems.
PLANT AND CELL PHYSIOLOGY
(2023)
Article
Plant Sciences
Song Zhang, Kailu Tang, Qiujing Yan, Xingyue Li, Liangliang Shen, Wenda Wang, Yi-Kun He, Tingyun Kuang, Guangye Han, Jian-Ren Shen, Xing Zhang
Summary: This article describes the structure of a photosystem I supercomplex in the moss Physcomitrium patens, revealing the assembly process mediated by Lhcb9 and providing important information on energy transfer pathways.