期刊
CHEMBIOCHEM
卷 16, 期 18, 页码 2615-2623出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.201500377
关键词
genomics; lantibiotics; natural products; Streptomyces; type IV lanthipeptide
资金
- ERA-IB GenoDrug (BMBF) [FKZ 0315930]
- German Center for Infection Research (DZIF) [TTU 09.802, TTU 09.704]
- Cluster of Excellence (UniCat-unifying concepts in catalysis)
- Novo Nordisk Foundation
- NNF Center for Biosustainability [New Bioactive Compounds] Funding Source: researchfish
- Novo Nordisk Fonden [NNF10CC1016517] Funding Source: researchfish
Lanthipeptides are ribosomally synthesized and post-translationally modified microbial secondary metabolites. Here, we report the identification and isolation of streptocollin from Streptomyces collinus Tu 365, a new member of class IV lanthipeptides. Insertion of the constitutive ermE* promoter upstream of the lanthipeptide synthetase gene stcL resulted in peptide production. The streptocollin gene cluster was heterologously expressed in S. coelicolor M1146 and M1152 with 3.5- and 5.5-fold increased yields, respectively. The structure and ring topology of streptocollin were determined by high resolution MS/MS analysis. Streptocollin contains four macrocyclic rings, with one lanthionine and three methyllanthionine residues. To the best of our knowledge, this is the first report on the isolation of a class IV lanthipeptide in preparative amounts, and on the successful heterologous expression of a class IV lanthipeptide gene cluster.
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