NLR immune receptor–nanobody fusions confer plant disease resistance

New recognition specificity in a plant immune receptor by molecular engineering of its integrated domain

(2022) Stella Cesari et al.   Nature Communications

A general approach for stabilizing nanobodies for intracellular expression

(2022) John G Dingus et al.   eLife

A five-transgene cassette confers broad-spectrum resistance to a fungal rust pathogen in wheat

(2021) Ming Luo et al.   NATURE BIOTECHNOLOGY

The allelic rice immune receptor Pikh confers extended resistance to strains of the blast fungus through a single polymorphism in the effector binding interface

(2021) Juan Carlos De la Concepcion et al.   PLoS Pathogens

Two NLR immune receptors acquired high-affinity binding to a fungal effector through convergent evolution of their integrated domain

(2021) Aleksandra Białas et al.   eLife

Structural insights into the binding of nanobodies LaM2 and LaM4 to the red fluorescent protein mCherry

(2021) Ziying Wang et al.   PROTEIN SCIENCE

Functional diversification gave rise to allelic specialization in a rice NLR immune receptor pair

(2021) Juan Carlos De la Concepcion et al.   eLife

Optimizing the PBS1 Decoy System to Confer Resistance to Potyvirus Infection in Arabidopsis and Soybean

(2020) Sarah E. Pottinger et al.   MOLECULAR PLANT-MICROBE INTERACTIONS

Structure-based engineering of anti-GFP nanobody tandems as ultra-high-affinity reagents for purification

(2020) Ziyue Zhang et al.   Scientific Reports

The rice NLR pair Pikp-1/Pikp-2 initiates cell death through receptor cooperation rather than negative regulation

(2020) Rafał Zdrzałek et al.   PLoS One

Ligand-triggered allosteric ADP release primes a plant NLR complex

(2019) Jizong Wang et al.   SCIENCE

Protein engineering expands the effector recognition profile of a rice NLR immune receptor

(2019) Juan Carlos De la Concepcion et al.   eLife

An N-terminal motif in NLR immune receptors is functionally conserved across distantly related plant species

(2019) Hiroaki Adachi et al.   eLife

Lessons in Effector and NLR Biology of Plant-Microbe Systems

(2018) Aleksandra Białas et al.   MOLECULAR PLANT-MICROBE INTERACTIONS

Stacking three late blight resistance genes from wild species directly into African highland potato varieties confers complete field resistance to local blight races

(2018) Marc Ghislain et al.   PLANT BIOTECHNOLOGY JOURNAL

Specific recognition of two MAX effectors by integrated HMA domains in plant immune receptors involves distinct binding surfaces

(2018) Liwei Guo et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Comparative analysis of plant immune receptor architectures uncovers host proteins likely targeted by pathogens

(2016) Panagiotis F. Sarris et al.   BMC BIOLOGY

Integration of decoy domains derived from protein targets of pathogen effectors into plant immune receptors is widespread

(2016) Thomas Kroj et al.   NEW PHYTOLOGIST

Using decoys to expand the recognition specificity of a plant disease resistance protein

(2016) S. H. Kim et al.   SCIENCE

A Receptor Pair with an Integrated Decoy Converts Pathogen Disabling of Transcription Factors to Immunity

(2015) Clémentine Le Roux et al.   CELL

A Plant Immune Receptor Detects Pathogen Effectors that Target WRKY Transcription Factors

(2015) Panagiotis F. Sarris et al.   CELL

Tomato I2 Immune Receptor Can Be Engineered to Confer Partial Resistance to the OomycetePhytophthora infestansin Addition to the FungusFusarium oxysporum

(2015) Artemis Giannakopoulou et al.   MOLECULAR PLANT-MICROBE INTERACTIONS

Structural basis of pathogen recognition by an integrated HMA domain in a plant NLR immune receptor

(2015) A Maqbool et al.   eLife

The “sensor domains” of plant NLR proteins: more than decoys?

(2015) Chih-Hang Wu et al.   Frontiers in Plant Science

Species-wide Genetic Incompatibility Analysis Identifies Immune Genes as Hot Spots of Deleterious Epistasis

(2014) Eunyoung Chae et al.   CELL

Single Amino Acid Mutations in the Potato Immune Receptor R3a Expand Response to Phytophthora Effectors

(2014) María Eugenia Segretin et al.   MOLECULAR PLANT-MICROBE INTERACTIONS

A robust pipeline for rapid production of versatile nanobody repertoires

(2014) Peter C Fridy et al.   NATURE METHODS

The powdery mildew resistance genePm8derived from rye is suppressed by its wheat orthologPm3

(2014) Severine Hurni et al.   PLANT JOURNAL

A Golden Gate Modular Cloning Toolbox for Plants

(2014) Carola Engler et al.   ACS Synthetic Biology

A novel conserved mechanism for plant NLR protein pairs: the “integrated decoy” hypothesis

(2014) Stella Cesari et al.   Frontiers in Plant Science

Agroinfiltration and PVX Agroinfection in Potato and Nicotiana benthamiana

(2014) Juan Du et al.   Jove-Journal of Visualized Experiments

Nanobodies: Natural Single-Domain Antibodies

(2013) Serge Muyldermans   Annual Review of Biochemistry

The Rice Resistance Protein Pair RGA4/RGA5 Recognizes the Magnaporthe oryzae Effectors AVR-Pia and AVR1-CO39 by Direct Binding

(2013) S. Cesari et al.   PLANT CELL

Pivoting the Plant Immune System from Dissection to Deployment

(2013) J. L. Dangl et al.   SCIENCE

Arms race co-evolution ofMagnaporthe oryzae AVR-Pikand ricePikgenes driven by their physical interactions

(2012) Hiroyuki Kanzaki et al.   PLANT JOURNAL

ggplot2: Elegant Graphics for Data Analysis

(2011) Cedric Ginestet   JOURNAL OF THE ROYAL STATISTICAL SOCIETY SERIES A-STATISTICS IN SOCIETY

A Modular Cloning System for Standardized Assembly of Multigene Constructs

(2011) Ernst Weber et al.   PLoS One

Functional stacking of three resistance genes against Phytophthora infestans in potato

(2011) Suxian Zhu et al.   TRANSGENIC RESEARCH

NB-LRR proteins: pairs, pieces, perception, partners, and pathways

(2010) Timothy K Eitas et al.   CURRENT OPINION IN PLANT BIOLOGY

Modulation of protein properties in living cells using nanobodies

(2009) Axel Kirchhofer et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Association Genetics Reveals Three Novel Avirulence Genes from the Rice Blast Fungal Pathogen Magnaporthe oryzae

(2009) K. Yoshida et al.   PLANT CELL

To Nibble at Plant Resistance Proteins

(2009) F. L. W. Takken et al.   SCIENCE

Two Adjacent Nucleotide-Binding Site-Leucine-Rich Repeat Class Genes Are Required to Confer Pikm-Specific Rice Blast Resistance

(2008) I. Ashikawa et al.   GENETICS