期刊
JOURNAL OF VIROLOGICAL METHODS
卷 229, 期 -, 页码 24-26出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jviromet.2015.12.002
关键词
Maculavirus; BmMLV; BEVS; BmNPV; Mammalian cell line
资金
- JSPS KAKENHI [20780045, 25450482]
- Grants-in-Aid for Scientific Research [25450482, 20780045] Funding Source: KAKEN
Bombyx mori-derived cell lines are generally used for Bombyx mori nucleopolyhedrovirus (BmNPV)-based baculovirus expression vector system (BEVS). However, almost all of the B. mori-derived cell lines are persistently infected with Bombyx mori macula-like virus (BmMLV). In this study, nontarget mammalian cell lines were exposed to BmMLV, and their susceptibility was investigated. Real-time PCR showed that viral RNA in virus-inoculated nine mammalian cell lines decreased sharply at 7 days postinfection. Also, there was no significant effect on cell viability of mammalian cells after inoculation with BmMLV. These findings indicate that mammalian cell lines used in this study are not permissive to BmMLV, and BmMLV contamination might not affect the safety aspect of BmNPV-based BEVS. (C) 2015 Elsevier B.V. All rights reserved.
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