Article
Microbiology
Dina B. Salama, Ragab M. Fereig, Hanan H. Abdelbaky, Moshera S. Shahat, Waleed M. Arafa, Shawky M. Aboelhadid, Adel E. A. Mohamed, Samy Metwally, Osama Abas, Xun Suo, Nishith Gupta, Caroline F. Frey
Summary: This study investigated the infection rates of Toxoplasma gondii and Neospora caninum in dogs and cats in Egypt. The results showed that 23.3% of dogs were exposed to T. gondii, while 5.8% had N. caninum-specific antibodies. In cats, the exposure rate to T. gondii was 9.8%, and 3.4% had N. caninum-specific antibodies. Factors such as age and breed were significantly associated with T. gondii antibody positivity in dogs.
Article
Microbiology
Ragab M. Fereig, Hanan H. Abdelbaky, Amira M. Mazeed, El-Sayed El-Alfy, Somaya Saleh, Mosaab A. Omar, Abdullah F. Alsayeqh, Caroline F. Frey
Summary: The prevalence of antibodies to Neospora caninum and Toxoplasma gondii in raw milk samples from different ruminants and Egyptian governorates was estimated. The study found a seroprevalence of 15.4% for both N. caninum and T. gondii antibodies in bulk milk samples, and 25.7% and 14% for individual milk samples, respectively. The presence of DNA was also detected for both parasites. Pearson correlation analysis showed a strong correlation between bulk and individual samples for T. gondii and a moderately strong correlation for N. caninum.
Article
Agriculture, Dairy & Animal Science
Helga Waap, Andrea Baerwald, Telmo Nunes, Gereon Schares
Summary: This study evaluated the seroprevalence and risk factors for Neospora caninum and Toxoplasma gondii in naturally exposed cattle in Portugal. The results showed a low but widespread seroprevalence of T. gondii, while N. caninum had a seroprevalence similar to other Mediterranean countries. Climate factors and management practices were significant predictors of infection for both parasites. The study highlights the importance of considering neosporosis in the differential diagnosis of cattle reproductive disorders and informing source attribution models for human toxoplasmosis.
Article
Biochemistry & Molecular Biology
Luisa Berna, Pablo Marquez, Andres Cabrera, Gonzalo Greif, Maria E. Francia, Carlos Robello
Summary: The study reveals errors in the assembly of the Neospora caninum genome and significant chromosomal rearrangements between Neospora caninum and Toxoplasma gondii, leading to a reduction in their karyotype. Additionally, more than 500 new genes were identified through reannotation of the Neospora caninum genome.
Article
Veterinary Sciences
Qiu-Yan Lv, He-Liang Zheng, Wen-He Yang, Guo-Hua Liu
Summary: The study investigated the genotypes of Toxoplasma gondii and Neospora caninum in domestic ducks in Hunan province, China. Results showed low infection rates and highlighted the importance of a One Health approach to managing toxoplasmosis.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Agriculture, Dairy & Animal Science
Lei Ma, Shuai Li, Yongbin Zhang, Ziru Wen
Summary: The study revealed a higher seroprevalence of N. caninum in dairy cows in Hebei province, China, compared to previous data, and it is the first to detect T. gondii and N. caninum antibodies in the vast areas of Hebei province.
ANIMAL BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Eva Bartova, Jirina Markova, Jana Sedlackova, Hana Band'ouchova, Karol Racka
Summary: In this study, wild bats were tested for the presence of selected parasites. The results showed that Toxoplasma gondii was detected in 1% of bats, while Neospora caninum was not found in any of the bats. Encephalitozoon spp. was detected in 25% of bats, including three different species. This is the first study to report a relatively high positivity of Encephalitozoon spp. in vespertilionid bats from Central Europe and worldwide.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Jamal Gharekhani, Mohammad Yakhchali, Asghar Afshari, Maryam Adabi
Summary: The study conducted a comprehensive evaluation of Neospora, Toxoplasma, and Brucella infections in milk samples from dairy farms in Hamedan province, Iran for the first time. Results showed that milk quality was affected by infections. Control measures such as regulating animal age, herd size, and animal species, as well as health education and milk pasteurization, can help reduce the spread of infectious diseases.
Article
Parasitology
Zixuan Feng, Huifang Ling, Zifu Zhu, Yanqun Pei, Zhepeng Sun, Xianmei Wang, Lifang Wang, Qun Liu, Jing Liu
Summary: This study screened for a specific antigen, TgGRA54, through immunoprecipitation-mass spectrometry (IP-MS), and established an indirect enzyme-linked immunosorbent assay (ELISA) based on the recombinant protein for detecting Toxoplasma gondii infection. Compared with the indirect immunofluorescent antibody test (IFAT), the ELISA method based on rTgGRA54 showed a 100% positive coincidence rate and an 80.95% negative coincidence rate. Therefore, the recombinant protein, TgGRA54, is a good diagnostic antigen for T. gondii.
PARASITOLOGY RESEARCH
(2023)
Review
Immunology
Ragab M. Fereig, Mosaab A. Omar, Abdullah F. Alsayeqh
Summary: Toxoplasmosis and neosporosis are important protozoan diseases worldwide, and controlling these diseases is currently challenging. Understanding host-parasite interactions and host strategies to combat infections can aid in developing effective control measures, including vaccines. Macrophages and the production of interleukin-12 (IL-12) and interferon gamma (IFN-gamma) play a crucial role in successful vaccine candidates. However, identifying potential vaccine candidates is a time-consuming process.
Review
Immunology
Ragab M. Fereig, Hanan H. Abdelbaky, El-Sayed El-Alfy, Mohamed El-Diasty, Ahmed Elsayed, Hassan Y. A. H. Mahmoud, Alsagher O. Ali, Abdulrahman Ahmed, Ehab Mossaad, Abdullah F. Alsayeqh, Caroline F. Frey
Summary: The seroprevalence of Toxoplasma gondii and Neospora caninum in recently imported camels from Sudan was investigated. The study found significant differences in infection rates between the two study sites and/or sampling periods. Additionally, a systematic review revealed the overall seroprevalence of T. gondii and N. caninum in camels worldwide.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Ragab M. Fereig, Gamal Wareth, Hanan H. Abdelbaky, Amira M. Mazeed, Mohamed El-Diasty, Adel Abdelkhalek, Hassan Y. A. H. Mahmoud, Alsagher O. Ali, Abdelrahman El-tayeb, Abdullah F. Alsayeqh, Caroline F. Frey
Summary: Toxoplasmosis, neosporosis, and brucellosis are common diseases causing infectious abortion and economic losses in farm animals. The study in Egypt found the highest seroprevalence rate for T. gondii in sheep and goats, with only antibodies to Brucella spp. associated with recent abortion. These findings could guide the development of more efficient control measures.
Article
Agriculture, Dairy & Animal Science
Jinfang Yang, Jingkai Ai, Tongsheng Qi, Xiaomin Ni, Zichun Xu, Liangting Guo, Yali Sun, Ying Li, Ming Kang, Jixu Li
Summary: This study establishes a record of the seroprevalence and molecular epidemiology of T. gondii and N. caninum infection in stray cats and dogs in the Qinghai-Tibetan Plateau Area. The results demonstrate the presence of both parasites in the tested animals and highlight the importance of stray cats and dogs in the transmission and prevalence of these infections.
Article
Microbiology
Stefania Zanet, Fabrizia Veronesi, Giuseppe Giglia, Carolina Raquel Pinto Baptista, Giulia Morganti, Maria Teresa Mandara, Rachele Vada, Luis Manuel Madeira De Carvalho, Ezio Ferroglio
Summary: The prevalence of cyst-forming parasites Toxoplasma gondii and Neospora caninum was assessed in migratory and sedentary birds of prey. Molecular and histopathological analysis were conducted on skeletal muscle and myocardium samples from 159 birds of prey from Central Italy, revealing a prevalence of 23.27% for T. gondii and 5.66% for N. caninum.
Article
Veterinary Sciences
Xiao-Hui Hu, Shi-Chen Xie, Qin-Li Liang, Li-Xiu Sun, Zhao Li, Jian-Fa Yang, Xing-Quan Zhu, Feng-Cai Zou, Jun-Jun He
Summary: This study investigated the seroprevalence of Toxoplasma gondii and Neospora caninum in black goats in Yunnan Province, China. The results showed that these two parasites were prevalent in black goats, indicating the need for measures to control them and reduce economic losses.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Microbiology
Ruenruetai Udonsom, Yoshifumi Nishikawa, Ragab M. Fereig, Thitirat Topisit, Natchakorn Kulkaweewut, Supitcha Chanamrung, Charoonluk Jirapattharasate
Summary: This study investigated the seroprevalence of Toxoplasma gondii infection in elephants in Thailand and found that Asian elephants are widely exposed to the parasite, with higher infection rates observed in young elephants. However, the source of infection was not determined, highlighting the need for further research on the potential risk factors associated with toxoplasmosis.
Article
Microbiology
Ruenruetai Udonsom, Aongart Mahittikorn, Charoonluk Jirapattharasate
Summary: A study conducted in three provinces in southern Thailand revealed the presence of Theileria spp. and A. ovis infection in goats, with T. orientalis being identified for the first time in Thai goats. This study provides valuable information on the infection rates and genetic diversity of tick-borne pathogens in goats, which can aid in the development of effective control measures for preventing animal diseases in Thailand.
Article
Microbiology
Shengwei Ji, Onur Ceylan, Zhuowei Ma, Eloiza May Galon, Iqra Zafar, Hang Li, Yae Hasegawa, Mutlu Sevinc, Tatsunori Masatani, Aiko Iguchi, Osamu Kawase, Rika Umemiya-Shirafuji, Masahito Asada, Ferda Sevinc, Xuenan Xuan
Summary: This study investigated the presence of tick-borne pathogens in cattle ticks in five provinces of Turkey and performed molecular characterization. The results showed that 57.8% of the DNA pools tested positive for at least one pathogen, with Babesia bovis being the most frequently detected pathogen. This study also reported the first molecular detection of Rickettsia sp. Chad in Turkey.
Article
Biochemistry & Molecular Biology
Hasan Aydin, Suleyman Akocak, Nebih Lolak, Onder Yumrutas, Ugur Uslu, Ibrahim Bozgeyik, Fatih Uckardes, Selami Gunal, Onur Ceylan
Summary: In our study, we synthesized and tested the cytotoxic, antifungal, and larvicidal activities of bis-sulfonamide Schiff base derivatives. Among the 16 compounds tested, A1B2, A1B4, A4B2, A4B3, and A4B4 showed cytotoxic activity against lung cancer cells. A2B1 and A2B2 exhibited the strongest antifungal activity against all tested fungi, while A1B1 had the highest larvicidal effect. These results suggest that these bis-substituted compounds could be promising pharmacophores for various diseases.
JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
(2023)
Article
Multidisciplinary Sciences
Onur Ceylan, Leticia Pereira Ungari, Gonca Soenmez, Cigdem Gul, Ceylan Ceylan, Murat Tosunoglu, Bengi Baycan, Lucia Helena O'Dwyer, Ferda Sevinc
Summary: Although Hepatozoon spp. is the most common intracellular protozoa infecting snakes, its occurrence in snakes from the Colubridae family in Turkiye is limited. This study investigated Hepatozoon spp. in venomous nose-horned vipers from Turkiye. Morphological and molecular methods were used, and positive results for Hepatozoon spp. were found in three snakes. A new species, Hepatozoon viperoi sp. nov., was identified and its genetic relationship with other Hepatozoon species was analyzed. This study expands our knowledge of Hepatozoon species in snakes and contributes to the understanding of haemogregarine protozoan parasites.
SCIENTIFIC REPORTS
(2023)
Article
Veterinary Sciences
Ugur Uslu, Ayse Evci, Hueseyin Koray Akdeniz, Onur Ceylan
Summary: Maggot debridement therapy (MDT), although losing its importance with the advent of antibiotics, has seen an increase in recent years for treating non-healing wounds. This case report investigates the efficacy of MDT on a post-operative infected wound in a 5-month-old Doberman dog. After multiple applications of MDT, the wound showed gradual healing and positive response.
ANKARA UNIVERSITESI VETERINER FAKULTESI DERGISI
(2023)
Article
Biochemistry & Molecular Biology
Hasan Aydin, Suleyman Akocak, Nebih Lolak, Ugur Uslu, Ahmet Sait, Serol Korkmaz, Ayse Parmaksiz, Onur Ceylan, Abdulbaki Aksakal
Summary: Multidrug resistance (MDR) poses challenges in the treatment of infections and cancer. Research and development studies are crucial in preventing MDR. This study focused on investigating the effects of multi-target triazene compounds on various activities related to bacteria, fungi, viruses, cytotoxicity, and larvicidal efficacy. Novel derivatives of 1,3-diaryltriazene-substituted sulfadiazine (SDZ) were synthesized and characterized, showing potential as multitargeted agents. These compounds exhibited antibacterial, antifungal, antiviral, cytotoxic, and larvicidal activities.
JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
(2023)
Article
Immunology
Junya Yamagishi, Onur Ceylan, Xuenan Xuan, Ferda Sevinc
Summary: In this study, the genome sequence of Babesia ovis isolated from an infected sheep in Turkey was obtained. The genome size was 7.81 Mbp with 3,419 protein-coding genes. Comparative genomics revealed the closest relationship between B. ovis and B. bovis, and identified 43 new ves genes.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Microbiology
Zhuowei Ma, Onur Ceylan, Eloiza May Galon, Uday Kumar Mohanta, Shengwei Ji, Hang Li, Thanh Thom Do, Rika Umemiya-Shirafuji, Shimaa Abd El-Salam El-Sayed, Iqra Zafar, Mingming Liu, Ferda Sevinc, Xuenan Xuan
Summary: This study investigated piroplasmids in ticks collected from small ruminants in Turkey. Several tick species were identified, with R. turanicus being the most common in goats and sheep. Molecular assay confirmed the presence of B. ovis and T. ovis, with calculated infection rates.
Article
Chemistry, Multidisciplinary
Hasan Aydin, Ugur Uslu, Suleyman Akocak, Nebih Lolak, Ahmet Sait, Serol Korkmaz, Ayse Parmaksiz, Onur Ceylan, Abdulbaki Aksakal
Summary: This study evaluated the efficacy of favipiravir, a new antiviral drug, and found it to be effective in terms of antibacterial, antifungal, cytotoxic, antiviral, and larvicidal activities. These findings suggest that favipiravir has the potential to be a multifunctional drug.
Article
Parasitology
Ruenruetai Udonsom, Onrapak Reamtong, Poom Adisakwattana, Supaluk Popruk, Charoonluk Jirapattharasate, Yoshifumi Nishikawa, Tawin Inpankaew, Jitbanjong Toompong, Manas Kotepui, Aongart Mahittikorn
Summary: This study identified specific immunoreactive proteins of Neospora caninum tachyzoites recognized by sera from cattle infected with various parasites. The identified antigens have potential applications in immunodiagnosis and vaccine development for Neospora caninum, as well as cross-reactive antigens for controlling diseases caused by apicomplexan protozoan parasites.
