Reprotoxic Effect of Tris(2,3-Dibromopropyl) Isocyanurate (TBC) on Spermatogenic Cells In Vitro

Tris(2,3-dibromopropyl) isocyanurate (TBC) belongs to the class of novel brominated flame retardants (NFBRs) that are widely used in industry. It has commonly been found in the environment, and its presence has been discovered in living organisms as well. TBC is also described as an endocrine disruptor that is able to affect male reproductive processes through the estrogen receptors (ERs) engaged in the male reproductive processes. With the worsening problem of male infertility in humans, a mechanism is being sought to explain such reproductive difficulties. However, so far, little is known about the mechanism of action of TBC in male reproductive models in vitro. Therefore, the aim of the study was to evaluate the effect of TBC alone and in cotreatment with BHPI (estrogen receptor antagonist), 17 beta-estradiol (E-2), and letrozole on the basic metabolic parameters in mouse spermatogenic cells (GC-1 spg) in vitro, as well as the effect of TBC on mRNA expression (Ki67, p53, Ppar gamma, Ahr, and Esr1). The presented results show the cytotoxic and apoptotic effects of high micromolar concentrations of TBC on mouse spermatogenic cells. Moreover, an increase in Ppar gamma mRNA levels and a decrease in Ahr and Esr1 gene expression were observed in GS-1spg cells cotreated with E-2. These results suggest the significant involvement of TBC in the dysregulation of the steroid-based pathway in the male reproductive cell models in vitro and may be the cause of the currently observed deterioration of male fertility. However, more research is needed to reveal the full mechanism of TBC engagement in this phenomenon.

Automated summary

Tris(2,3-dibromopropyl) isocyanurate (TBC), a novel brominated flame retardant, has been widely used in industry and found in the environment and living organisms. TBC acts as an endocrine disruptor through estrogen receptors, affecting male reproductive processes. This study evaluated the effect of TBC on mouse spermatogenic cells in vitro and observed cytotoxic and apoptotic effects. The dysregulation of the steroid-based pathway may be the cause of male infertility, but further research is needed to fully understand the mechanism of TBC involvement.