Aptamer-based silver nanoparticle decorated paper platform for electrochemical detection ovarian cancer biomarker PDGF

Early identification of ovarian cancer improves treatment options & survival rates. An electrochemical paper analytical device (ePAD) was reported in this study for detecting Platelet-derived growth factor (PDGF), an ovarian cancer biomarker. This research aims to create a quick nano-biosensing approach to recognize deadly ovarian cancer (OC) at an initial phase. The suggested ePAD has numerous positive characteristics, including simple, low-cost, repeatable, & disposable. The ePAD's working electrode contains silver nanoparticles. Furthermore, aptamers have improved the biosensor's functionality. Transmission electron microscopy (TEM), FTIR analysis, X-ray diffraction (XRD), UV-vis spectroscopy, & Zeta potential test were utilized to evaluate the silver nanoparticles' morphological, optical, elemental content, and phase analyses. This aptamer-modified ePAD helps amplify the designed platform's signal to detect PDGF. The interaction of aptamer & PDGF was discovered by lowering the current created by the contact with methylene blue (MB), an anionic indicator. The designed sensor demonstrated an optimum response & broad linearity of 0.01-10.0 & mu;g/ml for PDGF, with 0.01 & mu;g/ml of limit of detection (LOD).

Automated summary

An electrochemical paper analytical device (ePAD) was developed to detect the ovarian cancer biomarker PDGF, enabling early identification of ovarian cancer and improving treatment options and survival rates. The ePAD is a simple, low-cost, repeatable, and disposable biosensing approach that utilizes aptamers and silver nanoparticles as the working electrode. The designed sensor showed an optimal response with a broad linearity range and a low limit of detection for PDGF.