Article
Chemistry, Analytical
Monica Lin, Molly S. Blevins, Marta Sans, Jennifer S. Brodbelt, Livia S. Eberlin
Summary: In this study, solvent-based ambient ionization mass spectrometry techniques were used to investigate the extraction processes of lipids in biological tissues. The results suggest that the physicochemical properties of the solvent systems and the extraction time significantly influence the molecular extraction and detection of lipids.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Gwangbin Lee, Sangwon Cha
Summary: Nano-DESI MS has been explored in the forensic chemical analysis of ink directly from handwriting on paper, showing the potential for changing mass spectral profiles over time to reflect the differential distribution of analytes in a vertical direction. The study confirmed the capability of the depth-dependent analysis of nano-DESI MS.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2021)
Article
Chemistry, Analytical
Yajun Zheng, Yajie Huang, Qianqian Zuo, Yan Zhang, Yuhua Wu, Zhiping Zhang
Summary: In this study, a portable paper-based electrospray ionization (PPESI) technique was developed by using a piece of triangle paper and adsorbent packed into a modified disposable micropipette tip. The PPESI not only captures the features of paper spray and adsorbent for efficient suppression of sample matrixes, but also prevents rapid evaporation of spray solvent. The performance of PPESI can be optimized by adjusting the type and amount of adsorbent, paper substrate, spray solvent, and applied voltage. Compared to other sources, the PPESI coupled with mass spectrometry (MS) showed improved analytical sensitivity and spray duration.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Vishnu Shankar, Robert Tibshirani, Richard N. Zare
Summary: DESI-MSI has been increasingly used for detecting metabolites and lipids from human tissues and biofluids, but accurate computational and statistical methods are necessary to identify significant differences. MassExplorer is a flexible tool that helps researchers preprocess data, visualize structures, and select statistically significant metabolites.
Article
Chemistry, Analytical
Yuze Li, Junyu Chen, Lingwei Meng, Liuying He, Huihui Liu, Caiqiao Xiong, Zongxiu Nie
Summary: The MasSpec Pointer is a wireless pocket-size device powered by a 3.7 V polymer Li battery, which can be used for ambient ionization mass spectrometry. It has stable discharge and good quantification performance, allowing for quick testing of various samples in both positive and negative ion modes. The device can detect pesticides, chemicals, and other substances without any modification, and has a detection limit lower than 0.1 ng/mL for atrazine.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
G. Asher Newsome, Kathleen M. Martin
Summary: This study examines selected Native American woven woodsplint basketry created between 1870 and 1983 to recover traditional knowledge on their manufacture by identifying dyes or colorants. An ambient mass spectrometry system is used to non-invasively sample from intact objects, with no cutting or exposure to liquid. The analysis process involves thermal desorption, gas phase analysis, and photoionization for real-time identification of dye molecules.
ANALYTICAL CHEMISTRY
(2023)
Review
Biochemical Research Methods
Lanaia Itala Louzeiro Maciel, Ricardo Alves Bernardo, Rafael Oliveira Martins, Almir Custodio Batista Jr, Joao Victor Ataide Oliveira, Andrea Rodrigues Chaves, Boniek Gontijo Vaz
Summary: The imaging of biological tissues using mass spectrometry imaging (MSI) techniques allows for the visualization of the distribution of various metabolites, drugs, lipids, and glycans in complex samples. MSI methods like DESI-MSI and MALDI-MSI provide high sensitivity and the ability to evaluate/visualize multiple analytes in a single sample, overcoming limitations of classical microscopy techniques. This review discusses the application of DESI-MSI and MALDI-MSI in evaluating exogenous and endogenous molecules in biological samples, providing technical insights and recent research findings.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Fangfang Lu, Yajun Zheng, Yuan Zhang, Qiang Ma, Zhiping Zhang
Summary: Portable sampling of target analytes in complex biofluid samples using paper-in-tip spray ionization enables efficient and high sensitivity mass spectrometric analysis of therapeutic drugs and proteins. This technique allows for a long and stable spray duration with minimal spray solvent, enabling quick analysis of complex samples with high quantitation precision at picogram-per-milliliter levels.
ANALYTICAL METHODS
(2022)
Article
Biochemistry & Molecular Biology
Sangwon Cha, Gyouwoong Jun, Yougyeong Park, Sung Jun An, Donghoon Lee
Summary: Nanospray desorption electrospray ionization (nano-DESI) was used to analyze the metabolite fingerprints on Hypericum leaves. The intensity changes of key metabolites over time were observed through full mass scan experiments and monitored using selected ion monitoring and pseudo-selected reaction monitoring experiments. The correlation between the time-dependent changes in the metabolite fingerprints and depth-wise metabolite distribution was examined by removing the surface layers of the leaves multiple times.
Article
Multidisciplinary Sciences
Margot De Spiegeleer, Vera Plekhova, Jozefien Geltmeyer, Ella Schoolaert, Beata Pomian, Varoon Singh, Kathleen Wijnant, Kimberly De Windt, Volter Paukku, Alexander De Loof, Inge Gies, Nathalie Michels, Stefaan De Henauw, Marilyn De Graeve, Karen De Clerck, Lynn Vanhaecke
Summary: In recent years, ambient ionization mass spectrometry (AIMS) has allowed for direct analysis of biofluid metabolome. However, AIMS procedures are hindered by analytical and practical limitations, which affect metabolome coverage. This study aimed to develop biofluid-specific metabolome sampling membranes (MetaSAMPs) to improve AIMS. Customized MetaSAMPs made of hydrophilic and lipophilic polymers demonstrated superior metabolome coverage and transport stability compared to crude biofluid analysis. This new approach was successfully validated and has potential applications in metabolic health stratification.
Article
Environmental Sciences
Ricardo Alves Bernardo, Jean Carlos Pereira Sousa, Matheus Gallimberti, Fernando Barbosa Junior, Boniek Gontijo Vaz, Andrea Rodrigues Chaves
Summary: Concerns over human health risks due to the use of bisphenol A in thermal papers have led to its replacement by bisphenol S. Analyzing bisphenols typically involves laborious and time-consuming steps, but a new paper spray ionization mass spectrometry (PSI-MS) method offers a faster and efficient alternative. The study found that bisphenol S concentrations in BPA-free thermal papers ranged from 1.36 to 6.77 mu g g(-1), while BPA concentrations in all samples of thermal paper ranged from 6.56 to 16.4 mu g g(-1).
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Physics, Atomic, Molecular & Chemical
Ashley R. Stelmack, William L. Fatigante, Shahnaz Mukta, Phoebe C. Clowser, Jessica M. Holtz, Christopher C. Mulligan
Summary: This study systematically characterized the performance of paper spray ionization-mass spectrometry (PSI-MS) under variable environmental conditions, finding that wind speeds greater than 3 m/s had the most significant impact on PSI-MS spectral attributes, while moderate ambient temperature and %RH produced the best spectral performance.
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
(2022)
Article
Food Science & Technology
Yi-Tzu Cho, Hung Su, Ching-Ying Wu, Jingyueh Jeng, Chi-Wei Lee, Deng-Chyang Wu, Tiao-Lai Huang, Jentaie Shiea
Summary: This study aimed to develop an efficient and non-invasive method for monitoring drugs and their metabolites through human skin. By combining probe sampling with thermal desorption-electrospray ionization tandem mass spectrometry, trace amounts of terbinafine secreted on patients' skin after ingestion could be characterized and monitored for 8 weeks. The detection and location of terbinafine through the skin suggest that this method is useful for rapidly and noninvasively collecting molecular information of ingested drugs for pharmacokinetic studies.
JOURNAL OF FOOD AND DRUG ANALYSIS
(2022)
Review
Chemistry, Analytical
Sarmila Amatya Shrestha, Sangwon Cha
Summary: The paper reviews the application of ambient desorption/ionization mass spectrometry in the direct analysis of solid materials, covering various sampling and desorption processes. Real-world analysis cases include pharmaceuticals, food products, forensic analysis, and surface contaminant analysis. ADI techniques provide efficient and simplified methods for direct desorption and ionization of analytes from untreated samples under ambient conditions.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Fengjian Chu, Gaosheng Zhao, Wangyu Li, Wei Wei, Weiwei Chen, Zihan Ma, Zhan Gao, Nazifi Sani Shuaibu, Jikui Luo, Bingwen Yu, Hongru Feng, Yuanjiang Pan, Xiaozhi Wang
Summary: A microwave plasma torch-based ion/molecular reactor (MPTIR) has been developed for creating and monitoring rapid oxidation reactions without the addition of external oxidants or catalysts. The oxidation products from the MPTIR match those of the long-term environmental oxidative process, and two unreported environmental compounds were identified in actual water samples using this device.
ANALYTICAL CHEMISTRY
(2022)
Article
Substance Abuse
Iria Gonzalez-Marino, Jose Antonio Baz-Lomba, Nikiforos A. Alygizakis, Maria Jesus Andres-Costa, Richard Bade, Anne Bannwarth, Leon P. Barron, Frederic Been, Lisa Benaglia, Jean-Daniel Berset, Lubertus Bijlsma, Igor Bodik, Asher Brenner, Andreas L. Brock, Daniel A. Burgard, Erika Castrignano, Alberto Celma, Christophoros E. Christophoridis, Adrian Covaci, Olivier Delemont, Pim de Voogt, Damien A. Devault, Mario J. Dias, Erik Emke, Pierre Esseiva, Despo Fatta-Kassinos, Ganna Fedorova, Konstantinos Fytianos, Cobus Gerber, Roman Grabic, Emma Gracia-Lor, Stefan Gruener, Teemu Gunnar, Evroula Hapeshi, Ester Heath, Bjoern Helm, Felix Hernandez, Aino Kankaanpaa, Sara Karolak, Barbara Kasprzyk-Hordern, Ivona Krizman-Matasic, Foon Yin Lai, Wojciech Lechowicz, Alvaro Lopes, Miren Lopez de Alda, Ester Lopez-Garcia, Arndis S. C. Love, Nicola Mastroianni, Gillian L. McEneff, Rosa Montes, Kelly Munro, Thomas Nefau, Herbert Oberacher, Jake W. O'Brien, Reinhard Oertel, Kristin Olafsdottir, Yolanda Pico, Benedek G. Plosz, Fabio Polesel, Cristina Postigo, Jose Benito Quintana, Pedram Ramin, Malcolm J. Reid, Jack Rice, Rosario Rodil, Noelia Salgueiro-Gonzalez, Sara Schubert, Ivan Senta, Susana M. Simoes, Maja M. Sremacki, Katarzyna Styszko, Senka Terzic, Nikolaos S. Thomaidis, Kevin Thomas, Ben J. Tscharke, Robin Udrisard, Alexander L. N. van Nuijs, Viviane Yargeau, Ettore Zuccato, Sara Castiglioni, Christoph Ort
Article
Biochemical Research Methods
William L. Fatigante, Shahnaz Mukta, Zachary E. Lawton, Alessandra M. Bruno, Angelica Traub, Alyssa J. Gasa, Ashley R. Stelmack, Christina R. Wilson-Frank, Christopher C. Mulligan
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2020)
Article
Chemistry, Analytical
Daniel S. Burr, William L. Fatigante, Jemima A. Lartey, Wongi Jang, Ashley R. Stelmack, Noah W. McClurg, Jean M. Standard, Jamie R. Wieland, Jun-Hyun Kim, Christopher C. Mulligan, Jeremy D. Driskell
ANALYTICAL CHEMISTRY
(2020)
Article
Neurosciences
Katharina Huefner, Matyas Galffy, Jonas Egeter, Johannes M. Giesinger, Kathrin Arnhard, Herbert Oberacher, Johanna M. Gostner, Dietmar Fuchs, Barbara Sperner-Unterweger
Review
Spectroscopy
Kenyon Evans-Nguyen, Ashley R. Stelmack, Phoebe C. Clowser, Jessica M. Holtz, Christopher C. Mulligan
Summary: Mass spectrometry is widely used in forensic chemistry laboratories, with efforts to develop ruggedized, fieldable instruments for on-site analysis. Testing samples in the field is of particular interest in forensic science, homeland security, and defense applications. Mass spectrometry is well suited for providing definitive answers with low false positive rates in homeland security and military applications.
MASS SPECTROMETRY REVIEWS
(2021)
Article
Physics, Atomic, Molecular & Chemical
Ashley R. Stelmack, William L. Fatigante, Shahnaz Mukta, Phoebe C. Clowser, Jessica M. Holtz, Christopher C. Mulligan
Summary: This study systematically characterized the performance of paper spray ionization-mass spectrometry (PSI-MS) under variable environmental conditions, finding that wind speeds greater than 3 m/s had the most significant impact on PSI-MS spectral attributes, while moderate ambient temperature and %RH produced the best spectral performance.
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
(2022)
Article
Chemistry, Analytical
Miriam Stempfer, Vera Reinstadler, Anna Lang, Herbert Oberacher
Summary: The study found that recreational cannabis may be contaminated with pesticides, mycotoxins, and synthetic cannabinoids, posing a major threat to public health. A screening workflow using non-targeted LC MS/MS identified contaminants including synthetic cannabinoids, pesticide residues, and mycotoxins in seized cannabis samples.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Biochemistry & Molecular Biology
Lisa Bartenbach, Thomas Karall, Jakob Koch, Markus Andreas Keller, Herbert Oberacher, Sabine Scholl-Buergi, Daniela Karall, Gregor Oemer, Daniela Baumgartner, Katharina Meinel, Safwat Aly, Irena Odri-Komazec, Ralf Geiger, Miriam Michel
Summary: The study analyzed amino acid and lipid metabolic pathways in Marfan syndrome (MFS) patients and found significant differences in serum levels between patients and healthy controls, with some metabolites associated with cardiovascular risk and deterioration of myocardial function. The results showed that patients had a higher risk of cardiac diseases and exhibited alterations in amino acid and lipid metabolic pathways.
Article
Biochemical Research Methods
Vera Reinstadler, Susanne Huber, Stefan Lierheimer, Michael Boettcher, Peter Ruescher, Martin Tanzer, Kurt Jenny, Adolf Zoll, Wilhelm Gruber, Herbert Oberacher
Summary: To accurately detect cannabis-impaired individuals, researchers have developed a simple and effective workflow for quantifying THC and its metabolites in oral fluid samples. This involves rinsing the oral cavity with a solution containing a citrate buffer, extracting the cannabinoid fraction through centrifugation, and quantifying them using liquid chromatography-tandem mass spectrometry. The workflow has been shown to be suitable for forensic and clinical cannabis testing, and the oral fluid-based cannabis test demonstrated excellent sensitivity and specificity in identifying individuals with THC presence in blood, with an accuracy of 97.9%.
BIOMEDICAL CHROMATOGRAPHY
(2023)
Article
Environmental Sciences
Hannes Schenk, Petra Heidinger, Heribert Insam, Norbert Kreuzinger, Rudolf Markt, Fabiana Naegele, Herbert Oberacher, Christoph Scheffknecht, Martin Steinlechner, Gunther Vogl, Andreas Otto Wagner, Wolfgang Rauch
Summary: Wastewater-based epidemiology has been widely used in Austria since April 2020 to monitor the SARS-CoV-2 pandemic. This study analyzed the viral concentrations in raw sewage to predict short-term hospitalization occupancy. The results showed the potential of using viral loads in sewage to forecast Covid-19 hospitalization occupancy.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Cell Biology
Natascha Brigo, Emely Neumaier, Christa Pfeifhofer-Obermair, Philipp Grubwieser, Sabine Engl, Sylvia Berger, Markus Seifert, Vera Reinstadler, Herbert Oberacher, Gunter Weiss
Summary: Macrophages can be polarized into pro-inflammatory or anti-inflammatory subtypes, depending on whether they are primed with interferon-gamma (IFN gamma) or interleukin-4 (IL-4). This polarization affects the enzymes produced by the macrophages, which in turn determine the host's response to infection. L-arginine, a substrate for these enzymes, plays a crucial role in this process. While IL-4 impairs host resistance to Salmonella enterica serovar Typhimurium (S.tm), little is known about its effects on unpolarized macrophages during infection.
Article
Biochemistry & Molecular Biology
Herbert Oberacher, Vera Reinstadler, Marco Kreidl, Michael A. Strays, Juliane Hollender, Emma L. Schymanski
Review
Chemistry, Analytical
Hilary M. Brown, Trevor J. McDaniel, Patrick W. Fedick, Christopher C. Mulligan
ANALYTICAL METHODS
(2020)