Article
Gastroenterology & Hepatology
Sarah K. McGill, Scott P. Commins, Anne F. Peery, Joseph Galanko, Temitope O. Keku, Nicholas J. Shaheen, Chelsea Anderson, Robert S. Sandler
Summary: A survey in the Southeastern US found that 31.4% of participants in a screening colonoscopy had elevated alpha-gal IgE antibody levels. Patients with elevated antibody levels did not experience more abdominal pain and their meat intake was not different from those without alpha-gal antibodies. These findings suggest that the phenotypic expression of alpha-gal syndrome may be influenced by other host factors.
AMERICAN JOURNAL OF GASTROENTEROLOGY
(2023)
Review
Medicine, General & Internal
Takaaki Sawada, Jun Kido, Keishin Sugawara, Kimitoshi Nakamura
Summary: Fabry disease (FD) is an X-linked inherited disorder caused by mutations in the GLA gene. Early detection of FD is crucial, and high-risk screening can be effective. Peripheral neurological manifestations are significant in young FD patients, and GLA sequencing can enhance detection rates.
Article
Oncology
Julien Serrier, Jean-Baptiste Davy, Benoit Dupont, Benedicte Clarisse, Jean-Jacques Parienti, Gautier Petit, Kathy Khoy, Yann Ollivier, Radj Gervais, Delphine Mariotte, Brigitte Le Mauff
Summary: This study compared the performances of an automated Fluoroenzyme-Immunoassay (FEIA) for screening anti-alpha-Gal IgE with a previously validated anti-cetuximab IgE ELISA. The results showed that FEIA had better discrimination of positive and negative patients, higher specificity, and a higher positive likelihood ratio compared to ELISA. The risk of severe HSR following a positive test was higher with FEIA, while the risk following a negative test was similar.
Article
Biochemistry & Molecular Biology
Yu Fang, Meihong Dong, Sander S. van Leeuwen, Lubbert Dijkhuizen, Xiangfeng Meng, Weifeng Liu
Summary: This study explored two α-glucosidases (MT31a1 and MT31a2) from Myceliophthora thermophila and characterized their biochemical properties. MT31a1 displayed high thermostability and was able to synthesize main functional IMOs components from maltose. MT31a2, on the other hand, preferred substrates with α-(1?3) and α-(1?4) linkages. Both enzymes show potential for the synthesis of prebiotic glucooligosaccharides.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Genetics & Heredity
K. Savostyanov, A. Pushkov, I Zhanin, N. Mazanova, S. Trufanov, A. Pakhomov, A. Alexeeva, D. Sladkov, A. Asanov, A. Fisenko
Summary: A selective screening for Fabry disease (FD) among Russian patients with hypertrophic cardiomyopathy (HCM) revealed a prevalence rate of 0.4%. Next-generation sequencing (NGS) was used to detect gene variants and biochemical indicators were analyzed. The study identified patients with atypical FD characterized by residual alpha-galactosidase A activity, low lyso-Gb3 concentrations, and minor extracardiac manifestations.
ORPHANET JOURNAL OF RARE DISEASES
(2022)
Article
Biochemistry & Molecular Biology
Vega Miguel-Ruano, Ivanna Rivera, Jelena Rajkovic, Kamila Knapik, Ana Torrado, Jose Manuel Otero, Elisa Beneventi, Manuel Becerra, Mercedes Sanchez-Costa, Aurelio Hidalgo, Jose Berenguer, Maria-Isabel Gonzalez-Siso, Jacobo Cruces, L. Maria Rua, A. Juan Hermoso
Summary: The novel thermostable esterase EstD11 from a hot spring metagenomic library exhibits broad substrate specificity and belongs to the HSL family. Structural analysis and crystallographic experiments revealed the hot-spots in the active site and a unique Met zipper lining, providing insights into its enzymatic activity and stability.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2021)
Article
Medicine, General & Internal
Berkan Armagan, Metin Oksul, Yusuf Ziya Sener, Alper Sari, Abdulsamet Erden, Goezde Kuebra Yardimci, Kadir Mutlu Hayran, Levent Kilic, Oemer Karadag, Ergun Baris Kaya, Sadberk Lale Tokgozoglu, Ali Ihsan Ertenli, Ali Akdogan
Summary: This study aimed to investigate the prevalence and causes of pulmonary hypertension (PH) in Behcet's disease (BD). The study found that PH is not rare in BD patients and is mainly due to left heart disease or pulmonary arterial involvement. BD patients with vascular involvement are at a higher risk of developing PH.
TURKISH JOURNAL OF MEDICAL SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Vincenza Gragnaniello, Alessandro P. Burlina, Giulia Polo, Antonella Giuliani, Leonardo Salviati, Giovanni Duro, Chiara Cazzorla, Laura Rubert, Evelina Maines, Dominique P. Germain, Alberto B. Burlina
Summary: The study conducted in northeastern Italy over 5.5 years showed that 22 males were confirmed to have a GLA gene variant, with an incidence of 1:7879 newborns. All positive patients were asymptomatic at the last visit, with plasma lyso-Gb(3) levels used for non-invasive monitoring.
Article
Biochemistry & Molecular Biology
Ewelina Sobstyl, Agnieszka Szopa, Malgorzata Olszowy-Tomczyk, Sebastian Gnat, Karolina Jafernik, Irena Maria Choma
Summary: HPLC and TLC profiling were conducted on leaf and fruit extracts of five species from the Schisandraceae family. Lignans and phenolic compounds were found in both fruits and leaves of all species. The highest abundance of lignans was detected in the fruit extract of Schisandra chinensis, with 15 compounds identified. The fruit extracts of S. chinensis and S. rubriflora exhibited exceptional antibacterial activity, while all fruit and leaf extracts showed enzyme-inhibiting abilities, except for the fruit extract of Kadsura japonica. Antioxidant activity was stronger in the leaf extracts compared to the fruit extracts, assessed using TLC-direct bioautography and spectrophotometric measurements.
CHEMISTRY & BIODIVERSITY
(2023)
Article
Multidisciplinary Sciences
Yiming Chen, Yijie Chen, Wenwen Ning, Wen Zhang, Liyao Li, Xiaoying Wang, Yixuan Yin, Huimin Zhang
Summary: This study evaluated the clinical predictive value of serum alpha-fetoprotein variants (AFP-L2, AFP-L3) in combination with maternal serum prenatal screening biomarkers in predicting fetal trisomy 21 and trisomy 18. The results showed that AFP-L2 and AFP-L3 can serve as good screening markers, and their combination with other markers has the best performance.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Sivan Kinreich, Jacquelyn L. Meyers, Adi Maron-Katz, Chella Kamarajan, Ashwini K. Pandey, David B. Chorlian, Jian Zhang, Gayathri Pandey, Stacey Subbie-Saenz de Viteri, Dan Pitti, Andrey P. Anokhin, Lance Bauer, Victor Hesselbrock, Marc A. Schuckit, Howard J. Edenberg, Bernice Porjesz
Summary: This study successfully predicted the tendency to develop Alcohol Use Disorder (AUD) using machine learning analysis of EEG measurements, SNP data, and family history. Results demonstrated higher prediction accuracy for female patients compared to males, and the youngest age group showed higher accuracy in prediction in both African American and European American samples. Additionally, having a maternal history of AUD significantly increased the prediction accuracy.
MOLECULAR PSYCHIATRY
(2021)
Article
Nutrition & Dietetics
Tarapong Srisongkram, Sasisom Waithong, Thaweesak Thitimetharoch, Natthida Weerapreeyakul
Summary: Diabetes mellitus is a significant risk factor for cardiovascular disease and mortality. This study screened 31 plant samples using in vitro methods and machine learning models, and identified Pinus kesiya, Croton oblongifolius, Parinari anamense, and Polyalthia evecta as potential sources for the development of anti-diabetic recipes. The study also found that the alpha-glucosidase inhibitory activity of Thai indigenous plants can be better predicted based on phytochemical constituents compared to alpha-amylase inhibitory activity. Furthermore, the study determined the contribution of different chemical compounds to the activity of these enzymes.
Article
Plant Sciences
Xiaoxi Zhang, Zexiong Chen, Caini Wang, Xian Zhou, Ning Tang, Weiwei Zhang, Feng Xu, Zhiwu Yang, Chengrong Luo, Yongling Liao, Jiabao Ye
Summary: This study identified and classified 76 HD-ZIP genes from the Zanthoxylum armatum genome, with ZaHDZ16 identified as a potential gene related to prickle development. These findings contribute to a better understanding of the HD-ZIP gene family and provide a foundation for further research on the function of genes related to prickle development in Zanthoxylum armatum.
Article
Biochemistry & Molecular Biology
Deena Nath Gupta, Vikram Dalal, Brajesh Kumar Savita, Md Shahid Alam, Anamika Singh, Mrugendra Gubyad, Dilip Kumar Ghosh, Pravindra Kumar, Ashwani Kumar Sharma
Summary: In this study, the Bacterioferritin comigratory protein family 1 Cys peroxiredoxin from Candidatus Liberibacter asiaticus (CLaBCP) was biochemically studied and screened for potential antibacterial molecules. The results showed that CLaBCP has enzymatic activity and undergoes structural changes at different pH and temperature ranges. A structure-based drug design method was used to identify more efficient molecules, and molecular dynamics studies confirmed their stability.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Biotechnology & Applied Microbiology
Abdelmageed M. M. Othman, Ali M. M. Elshafei, Maysa A. A. Elsayed, Gamil E. E. Ibrahim, Mohamed M. M. Hassan, Nayra S. S. Mehanna
Summary: The study purified alpha-galactosidase enzyme from Aspergillus niger NRC114 and determined its optimum pH, temperature, stability, and activation. The application of this enzyme can improve the antioxidant properties and volatile compound content of soymilk yogurt, indicating its importance in future research and various biological fields.
Review
Clinical Neurology
Michele Romoli, Eleonora Matteo, Ludovica Migliaccio, Mauro Gentile, Maria Giulia Mosconi, Giuseppe Maria Scura, Marcello Naccarato, Enrico Colangeli, Paolo Candelaresi, Vincenzo Andreone, Fabrizio Giammello, Rosa Fortunata Musolino, Cristina Dell'Aera, Federica Nicoletta Sepe, Edoardo Pronello, Leonardo Barbarini, Marcella Caggiula, Federica Rizzo, Marco Petruzzellis, Elisa Giorli, Maria Luisa Zedde, Sabrina Anticoli, Marilena Mangiardi, Mario Muto, Francesco Diana, Maria Vittoria De Angelis, Anna Digiovanni, Letizia Concari, Sara La Gioia, Maria Sessa, Sara Biguzzi, Francesco Cordici, Marco Longoni, Maria Ruggiero, Silvia Cenciarelli, Paolo Eusebi, Simona Sacco, Valeria Caso, Maurizio Paciaroni, Stefano Ricci, Andrea Zini, Danilo Toni, David Giannandrea
Summary: This nationwide observational study evaluated the efficacy and safety of thrombolysis preceded by dabigatran-reversal in acute ischemic stroke patients. The results showed that there was no significant difference in symptomatic intracranial hemorrhage, death, and good functional outcome between the reversal group and the control group. However, reversal treatment seemed to be associated with a slight increase in the risk of symptomatic intracranial hemorrhage.
EUROPEAN STROKE JOURNAL
(2023)
Article
Cardiac & Cardiovascular Systems
Stefano Ghio, Mauro Acquaro, Piergiuseppe Agostoni, Giuseppe Ambrosio, Erberto Carluccio, Vincenzo Castiglione, Davide Colombo, Michele D'Alto, Santo Delle Grottaglie, Frank Lloyd Dini, Michele Emdin, Martino Fortunato, Andrea Igoren Guaricci, Francesca Jacoangeli, Alberto M. Marra, Stefania Paolillo, Sivia Papa, Luca Vicini Scajola, Michele Correale, Alberto Palazzuoli
Summary: For a long time, cardiologists have underestimated the role of the right heart in heart failure caused by left heart disease. However, the importance of evaluating right ventricular structure and function in left heart failure is now well recognized and emphasized in the latest heart failure guidelines. Yet, there are still unanswered questions about which imaging techniques and parameters should be used to assess the severity of RV dysfunction, and whether less commonly used diagnostic tools have a role in this field.
HEART FAILURE REVIEWS
(2023)
Article
Cardiac & Cardiovascular Systems
Andrea M. D. 'Armini, Maurizio Pin, Anna Celentano, Leslie J. Te Masiglat, Ermelinda Borrelli, Benedetta Vanini, Catherine Klersy, Giuseppe Silvaggio, Cristian Monterosso, Alessia Alloni, Carlo Pellegrini, Stefano Ghio
Summary: This study showed that in patients with chronic thromboembolic pulmonary hypertension undergoing pulmonary endarterectomy, a higher number of treated pulmonary artery branches is associated with better hemodynamic and clinical outcomes after surgery.
INTERNATIONAL JOURNAL OF CARDIOLOGY
(2023)
Article
Cardiac & Cardiovascular Systems
Giovanna Rodio, Saverio Iacopino, Ennio C. Pisano, Valeria Calvi, Giovanni Rovaris, Massimiliano Marini, Massimo Giammaria, Fabrizio Caravati, Giampiero Maglia, Gabriele Zanotto, Paolo Della Bella, Mauro Biffi, Antonio Curnis, Massimiliano Maines, Daniela Orsida, Matteo Santamaria, Giovanni Bisignani, Matteo Baroni, Fabio Lissoni, Antonio Duca, Giovanni B. Forleo, Carlo Piemontese, Alberto De Salvia, Gennaro Miracapillo, Eduardo Celentano, Massimo Zecchin, Giovanni Luzzi, Daniele Giacopelli, Alessio Gargaro, Antonio D'Onofrio
Summary: This study aimed to assess the temporal association of decreasing thoracic impedance (TI) trends with ventricular tachycardia/fibrillation (VT/VF) episodes through a longitudinal analysis of daily remote monitoring data from implantable cardioverter-defibrillators (ICDs) and cardiac resynchronization therapy defibrillators (CRT-Ds). The study found that only in ICD patients, a decrease in TI was associated with VT/VF episodes, while there was no such association in CRT-D patients.
JOURNAL OF CARDIOVASCULAR ELECTROPHYSIOLOGY
(2023)
Review
Cardiac & Cardiovascular Systems
Manuel J. Richter, Federico Fortuni, Fawaz Alenezi, Michele D'Alto, Roberto Badagliacca, Nathan W. Brunner, Arie P. van Dijk, Philipp Douschan, Henning Gall, Stefano Ghio, Francesco Lo Giudice, Ekkehard Grunig, Francois Haddad, Luke Howard, Sudarshan Rajagopal, Niels Stens, Davide Stolfo, Dick H. J. Thijssen, Carmine Dario Vizza, Roham T. Zamanian, Liang Zhong, Werner Seeger, Hossein A. Ghofrani, Khodr Tello
Summary: A meta-analysis of 35 studies found that right atrial (RA) imaging assessment is a relevant prognostic marker in patients with pulmonary hypertension (PH). RA area, RA indexed area, RA peak longitudinal strain, and RA total emptying fraction were significantly associated with combined end-points including death, clinical worsening, and/or lung transplantation. RA volume and volume index showed marginal significant associations.
JOURNAL OF HEART AND LUNG TRANSPLANTATION
(2023)
Article
Clinical Neurology
Manuela De Michele, Marta Iacobucci, Federica Letteri, Chiara Andreoli, Svetlana Lorenzano, Rossella Canese, Luca Petraglia, Irene Berto, Oscar Gaetano Schiavo, Claudio Di Biasi, Danilo Toni
Summary: The study identified a 1.28-ppm peak possibly indicating neural progenitor cells in the post-stroke human brain using magnetic resonance spectroscopy.
NEUROLOGICAL SCIENCES
(2023)
Article
Clinical Neurology
Irene Escudero-Martinez, Magnus Thoren, Peter Ringleb, Ana Paiva Nunes, Manuel Cappellari, Viiu-Marika Rand, Piotr Sobolewski, Jose Egido, Danilo Toni, Shih-Yin Chen, Nicole Tsao, Niaz Ahmed
Summary: This study aimed to describe the incidence, risk factors, and outcomes of cerebral edema (CED) in patients with extensive ischemia. Results showed that age <50, high blood glucose, signs of acute infarct, hyperdense artery on baseline scans, and decreased level of consciousness were the most important predictors for moderate/severe CED. CED was associated with worse functional outcome and a higher risk of death at 3 months.
Article
Neuroimaging
Andrea Naldi, Giovanni Pracucci, Roberto Cavallo, Valentina Saia, Andrea Boghi, Piergiorgio Lochner, Ilaria Casetta, Fabrizio Sallustio, Andrea Zini, Enrico Fainardi, Manuel Cappellari, Rossana Tassi, Sandra Bracco, Guido Bigliardi, Stefano Vallone, Patrizia Nencini, Mauro Bergui, Salvatore Mangiafico, Danilo Toni
Summary: This study aimed to evaluate the outcomes, safety, and time intervals of mechanical thrombectomy (MT) in patients with in-hospital stroke (IHS) compared with out-of-hospital stroke (OHS) patients. The results showed that IHS patients had worse functional outcomes and higher mortality at 3 months. However, the recanalization rates and risk of symptomatic intracranial hemorrhage were similar. Improvements in the management and shorter time intervals are needed for IHS patients.
JOURNAL OF NEUROINTERVENTIONAL SURGERY
(2023)
Article
Clinical Neurology
Ettore Nicolini, Marta Iacobucci, Manuela De Michele, Antonio Ciacciarelli, Irene Berto, Luca Petraglia, Anne Falcou, Carlo Cirelli, Francesco Biraschi, Svetlana Lorenzano, Italo Linfante, Danilo Toni
Summary: Retrospective analysis of 185 cases of acute ischemic stroke patients showed that changes in the pattern of brain vasculature can predict the absence of reperfusion and failure of early neurological improvement after endovascular treatment. However, it is not able to predict poor 90-day outcome. In addition, these changes also predicted the risk of hemorrhagic transformation.
NEUROLOGICAL SCIENCES
(2023)
Article
Clinical Neurology
Ghil Schwarz, Angelo Cascio Rizzo, Marius Matusevicius, Giuditta Giussani, Paolo Invernizzi, Fabio Melis, Norbert Lesko, Danilo Toni, Elio Clemente Agostoni, Niaz Ahmed
Summary: This study compared the differences between selective and non-selective treatments in mild acute ischemic stroke through endovascular thrombectomy. The results showed that there were no significant differences in the safety and efficacy of acute reperfusion treatments between disabling and nondisabling patients. Similar treatment approaches can be adopted for both groups. However, randomized data are needed to determine the best reperfusion treatment for mild acute ischemic stroke.
Review
Medicine, General & Internal
Marialuisa Zedde, Manuela Napoli, Ilaria Grisendi, Federica Assenza, Claudio Moratti, Franco Valzania, Rosario Pascarella
Summary: The pathophysiology of lacunar infarction is a complex and debated field, with information mainly derived from histopathology, anatomical studies, and animal models. Recent advancements in neuroimaging techniques have allowed for a better understanding of the dynamic evolution of small vessel occlusion and the mechanisms of damage. The core-penumbra concept, initially explored in large vessel occlusions, has also been applied to lacunar stroke, leading to the identification of perfusion deficits in a subgroup of patients. This review aims to summarize the pathophysiological issues and the application of the core-penumbra hypothesis in lacunar stroke.
Review
Biochemistry & Molecular Biology
Marialuisa Zedde, Ilaria Grisendi, Federica Assenza, Gabriele Vandelli, Manuela Napoli, Claudio Moratti, Piergiorgio Lochner, David J. Seiffge, Fabrizio Piazza, Franco Valzania, Rosario Pascarella
Summary: Small vessel diseases (SVD) encompass various conditions that affect small arteries, arterioles, capillaries, and venules in the brain. One prominent example is cerebral amyloid angiopathy (CAA), characterized by the deposition of amyloid fragments in the walls of small cortical and leptomeningeal vessels. CAA often coexists with Alzheimer's Disease (AD), and both are associated with cerebrovascular events, cognitive impairment, and dementia. Despite the shared pathophysiological and histopathological features, the involvement of venules in both diseases has been overlooked.
Review
Biology
Fabrizio Guarracini, Alberto Preda, Eleonora Bonvicini, Alessio Coser, Marta Martin, Silvia Quintarelli, Lorenzo Gigli, Matteo Baroni, Sara Vargiu, Marisa Varrenti, Giovanni Battista Forleo, Patrizio Mazzone, Roberto Bonmassari, Massimiliano Marini, Andrea Droghetti
Summary: The difference between subcutaneous implantable cardioverter defibrillators (S-ICDs) and transvenous ICDs (TV-ICDs) lies in the extra-thoracic implantation of S-ICDs, without endovascular components. S-ICDs have been rapidly adopted due to their improved safety profile, especially among younger patients. Although S-ICDs show comparable efficacy, reliability, and safety outcomes to TV-ICDs, technical issues limit their employment, making them an alternative rather than a mainstream option.
Article
Clinical Neurology
Maurizio Paciaroni, Valeria Caso, Michele Romoli, Cecilia Becattini, Alexander Salerno, Costanza Rapillo, Fanny Simonnet, Davide Strambo, Isabella Canavero, Marialuisa Zedde, Rosario Pascarella, Sung-Il Sohn, Simona Sacco, Raffaele Ornello, Kristian Barlinn, Daniela Schoene, Jan Rahmig, Maria Giulia Mosconi, Ilaria Leone De Magistris, Andrea Alberti, Michele Venti, Giorgio Silvestrelli, Alfons Ciccone, Marina Padroni, Michele Laudisi, Andrea Zini, Luana Gentile, Odysseas Kargiotis, Georgios Tsivgoulis, Rossan Tassi, Francesca Guideri, Maurizio Acampa, Luca Masotti, Elisa Grifoni, Alessandro Rocco, Marina Diomedi, Theodore Karapanayiotides, Stefan T. Engelter, Alexandros A. Polymeris, Annaelle Zietz, Fabio Bandini, Pietro Caliandro, Giuseppe Reale, Marco Moci, Aurelia Zauli, Manuel Cappellari, Andrea Emiliani, Antonio Gasparro, Valeria Terruso, Marina Mannino, Elisa Giorli, Danilo Toni, Marco Andrighetti, Anne Falcou, Lina Palaiodimou, George Ntaios, Dimitrios Sagris, Efstathia Karagkiozi, Anastasia Adamou, Panagiotis Halvatsiotis, Yuriy Flomin, Umberto Scoditti, Antonio Genovese, Nemanja Popovic, Leonardo Pantoni, Francesco Mele, Nicola Molitierno, Piergiorgio Lochner, Alessandro Pezzini, Massimo Del Sette, Davide Sassos, Sotirios Giannopoulos, Maria Kosmidou, Evangelos Ntais, Enrico Maria Lotti, Vincenzo Mastrangelo, Alberto Chiti, Andrea Naldi, Peter Vanacker, Mario Ferrante, Vera Volodina, Michelangelo Mancuso, Nicola Giannini, Marco Baldini, Kostantinos Vadikolias, Sofia Kitmeridou, Carlo Emanuele Saggese, Tiziana Tassinari, Valentina Saia, Patrik Michel
Summary: This study compared the outcomes of mechanical heart valve (MHV) patients with acute ischemic stroke undergoing full dose heparin along with vitamin K antagonists (VKA) (bridging therapy group) and restarting VKA without heparin (nonbridging group). The results showed that patients in the bridging therapy group had a slightly higher risk of ischemic or hemorrhagic events compared to those in the nonbridging group.
EUROPEAN STROKE JOURNAL
(2023)
Article
Clinical Neurology
Marialuisa Zedde, Ilaria Grisendi, Federica Assenza, Gabriele Vandelli, Manuela Napoli, Claudio Moratti, Franco Valzania, Rosario Pascarella
Summary: The study presents a rare case of a male patient who developed lacunar ischemic stroke and atypical variant of PRES with chronic small vessel disease markers, as seen in the MRI findings. The association between PRES and ischemic stroke provides insights into the pathomechanisms of PRES.
NEUROLOGICAL SCIENCES
(2023)