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Novel combination method of wide-range serial sectioning and 3D reconstruction visualizing both macro-level dynamics and micro-level interactions in an attempt to analyze the female pelvic floor

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ANATOMICAL SCIENCE INTERNATIONAL
卷 98, 期 3, 页码 343-352

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SPRINGER
DOI: 10.1007/s12565-023-00710-0

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3D reconstruction; Anatomy; Histology; Methods; Pelvic floor

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This article introduces a method for combining wide-range serial sectioning and 3D reconstruction using an adult cadaver. The method overcomes the restrictions of conventional visualization methods and allows for non-destructive 3D visualization of various structures. It is particularly useful in meso-anatomy, a discipline that bridges the gap between macro-anatomy and micro-anatomy.
The present report presents details of the method for combining wide-range serial sectioning and 3D reconstruction using an adult cadaver. For several decades, anatomists have utilized a variety of non-destructive three-dimensional (3D) visualization methods to complement gross anatomical analysis methods. These include vascular casting for the visualization of vascular morphology and micro-CT for the visualization of bone morphology. However, these conventional methods are restricted by the properties and sizes of the target structures. Here, we introduce a method to conduct 3D reconstruction based on wide-range serial histological sections from adult cadavers, which overcomes previous restrictions. An attempt at 3D visualization of the female pelvic floor muscles provides a detailed description of the procedure. Supplemental video and 3D PDF files allow multifaceted observation of 3D images. Wide-range serial sectioning visualizes morphology beyond the scope of conventional methods, while 3D reconstruction enables non-destructive 3D visualization of any structure that can be observed on a histological section, including skeletal muscle, smooth muscle, ligaments, cartilage, connective tissue, blood vessels, nerves, lymph nodes, and glands. The novel combination of both methods is instrumental in meso-anatomy, a discipline intermediate between macro-anatomy and micro-anatomy.

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