期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 138, 期 11, 页码 3627-3630出版社
AMER CHEMICAL SOC
DOI: 10.1021/jacs.5b11876
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资金
- Austrian Science Foundation FWF [P27947, I1040]
- Austrian Science Fund (FWF) [P27947, I1040] Funding Source: Austrian Science Fund (FWF)
- Austrian Science Fund (FWF) [I 1040, P 27947] Funding Source: researchfish
A pronounced rate differentiation has been found for conformational rearrangements of individual nucleobases that occur during ligand recognition of the preQ(1) class-I riboswitch aptamer from Thermoanaerobacter tengcongensis. Rate measurements rely on the 2ApFold approach by analyzing the fluorescence response of riboswitch variants, each with a single, strategically positioned 2-aminopurine nucleobase substitution. Observed rate discrimination between the fastest and the slowest conformational adaption is 22-fold, with the largest rate observed for the rearrangement of a nucleoside directly at the binding site and the smallest rate observed for the 3'-unpaired nucleoside that stacks onto the pseudo knot-closing Watson Crick base pair. Our findings provide novel insights into how compact, prefolded RNAs that follow the induced-fit recognition mechanism adapt local structural elements in response to ligand binding on a rather broad time scale and how this process culminates in a structural signal that is responsible for efficient downregulation of ribosomal translation.
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