Article
Biochemistry & Molecular Biology
Jinglue Song, Xia Shen, Zhenyu Huang, Yun Liu, Long Cui, Xuewei Cui, Chen-Ying Liu
Summary: Toxins A and B of Clostridium difficile disrupt the Hippo pathway, leading to sequestration and inactivation of YAP and TAZ in colonic epithelial cells. Overexpression of YAP restores cell function and may serve as a potential therapeutic strategy for treating CDI.
JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
(2021)
Article
Multidisciplinary Sciences
Woon Yong Kwon, Gil Joon Suh, Yoon Sun Jung, Seung Min Park, Subi Oh, Sung Hee Kim, A. Rum Lee, Jeong Yeon Kim, Hayoung Kim, Kyung Ah Kim, Young Kim, Byoung Choul Kim, Taegyun Kim, Kyung Su Kim, Kiyoshi Itagaki, Carl J. Hauser
Summary: The study revealed a strong association between circulating ND6 levels in patients with septic shock and the development of secondary infection, as well as increased mortality rates. Suppression of PMN chemotaxis may play a key role in the increased susceptibility to secondary infection in these patients.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Gastroenterology & Hepatology
Salvador Lopez-Cardenas, Eva Torres-Martos, Juan Mora-Delgado, Juan Manuel Sanchez-Calvo, Marta Santos-Pena, Angel Zapata Lopez, Maria Dolores Lopez-Prieto, Salvador Perez-Cortes, Juan Carlos Alados
Summary: The study revealed that patients with detected presence of TcdB and CDT in feces exhibited greater disease severity and higher recurrence rates compared to those without detection. Simultaneous detection of both markers had a stronger impact on prognosis in cases of CDI.
Article
Pharmacology & Pharmacy
Giuseppe Esposito, Chiara Corpetti, Marcella Pesce, Luisa Seguella, Giuseppe Annunziata, Alessandro Del Re, Martina Vincenzi, Roberta Lattanzi, Jie Lu, Walter Sanseverino, Giovanni Sarnelli
Summary: The newly designed PEA-producing probiotic showed therapeutic potential in inhibiting colonic inflammation and restoring tight junction protein expression in a mouse model of CDI.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Multidisciplinary Sciences
Srijita Basak, Debashrito Deb, Utkarsh Narsaria, Tamalika Kar, Filippo Castiglione, Indraneel Sanyal, Pratap D. Bade, Anurag P. Srivastava
Summary: Clostridium difficile infection is a major health-associated infection with high incidence and mortality rates. A chimeric vaccine candidate was designed using immunoinformatics, which showed stability and reliability in silico and molecular dynamics simulations. Docking studies demonstrated stable interactions with immune receptors, and in silico modeling indicated competent expression in E. coli system for potential immune response.
SCIENTIFIC REPORTS
(2021)
Article
Oncology
Julia L. Drewes, Jie Chen, Nicholas O. Markham, Reece J. Knippel, Jada C. Domingue, Ada J. Tam, June L. Chan, Lana Kim, Madison McMann, Courtney Stevens, Christine M. Dejea, Sarah Tomkovich, John Michel, James R. White, Fuad Mohammad, Victoria L. Campodonico, Cody N. Heiser, Xinqun Wu, Shaoguang Wu, Hua Ding, Patricia Simner, Karen Carroll, Martha J. Shrubsole, Robert A. Anders, Seth T. Walk, Christian Jobin, Fengyi Wan, Robert J. Coffey, Franck Housseau, Ken S. Lau, Cynthia L. Sears
Summary: This study reveals the role of toxigenic Clostridioides difficile in promoting colorectal cancer, suggesting that chronic colonization with this pathogenic bacteria may drive tumor development through the induction of Wnt signaling, reactive oxygen species, and protumorigenic mucosal immune responses.
Article
Infectious Diseases
Miad Elahi, Haruyuki Nakayama-Imaohji, Masahito Hashimoto, Ayano Tada, Hisashi Yamasaki, Tamiko Nagao, Tomomi Kuwahara
Summary: The gut symbiont Bacteroides thetaiotaomicron (BT) suppresses Clostridium difficile (CD) toxin production by inhibiting polysaccharide metabolism pathways. Polysaccharide fractions derived from BT can suppress CD toxin production, with cell wall-associated glycans playing a key role in this inhibitory effect.
Review
Public, Environmental & Occupational Health
Peng An Khun, Thomas V. Riley
Summary: This review provides an overview of Clostridium difficile infection (CDI) in southeast Asia, including its prevalence, detection methods, antimicrobial susceptibility profiles, and the potential significance of a One Health approach. CDI is a common cause of hospital-acquired gastroenteritis worldwide, with higher rates observed in high-income countries. Lack of knowledge and awareness about CDI, along with antimicrobial abuse and inadequate education on appropriate usage, contribute to its evolution. Underreporting of CDI rates and misuse of antimicrobial agents pose a significant threat in regions like Indochina, while C. difficile RT 017 strain is endemic in southeast Asia.
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
(2022)
Article
Microbiology
Mahmoud Fayez, Waleed R. El-Ghareeb, Ahmed Elmoslemany, Saleem J. Alsunaini, Mohamed Alkafafy, Othman M. Alzahrani, Samy F. Mahmoud, Ibrahim Elsohaby
Summary: The study investigated the presence of Clostridium perfringens and Clostridioides difficile in camel minced meat samples collected from small butcher shops and supermarkets in Al-Ahsa Governorate, Saudi Arabia. It was found that most isolates exhibited resistance to at least one antimicrobial, with some showing multidrug resistance. This highlights the importance of enforcing food safety standards and conducting regular inspections in abattoirs, small butcher shops, and supermarkets.
Article
Immunology
Bhargavi Duvvuri, Al Anoud Baddour, Kevin D. Deane, Marie L. Feser, J. Lee Nelson, M. Kristen Demoruelle, Christian Lood
Summary: Elevated levels of total fMet in the circulation of RA patients are associated with disease activity, joint involvement, and the development of rheumatoid nodules. Total fMet levels can predict erosive disease and improve the prognostic ability of ACPA, suggesting a potential role as novel biomarkers for disease monitoring and prognosis in RA. Additionally, circulating mtNFPs induce neutrophil activation through FPR1-dependent mechanisms, indicating FPR1 as a potential therapeutic target for RA.
JOURNAL OF AUTOIMMUNITY
(2021)
Article
Medicine, General & Internal
Andrew M. Skinner, S. Tyler Phillips, Michelle M. Merrigan, Kevin J. O'Leary, Susan P. Sambol, Farida Siddiqui, Lance R. Peterson, Dale N. Gerding, Stuart Johnson
Summary: Most pathogenic strains of C. difficile possess two large molecular weight single unit toxins that disrupt the actin cytoskeleton of intestinal epithelial cells, leading to diarrhea. While TcdB alone can cause disease, TcdA-/TcdB+ mutant strains have been shown to be responsible for C. difficile infection, which are typically mild in nature.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Cell Biology
Hung Chan, Qing Li, Xiansong Wang, Wing Yingzhi Liu, Wei Hu, Judeng Zeng, Chuan Xie, Thomas Ngai Yeung Kwong, Idy Hiu Ting Ho, Xiaodong Liu, Huarong Chen, Jun Yu, Ho Ko, Raphael Chiu Yeung Chan, Margaret Ip, Tony Gin, Alfred Sze Lok Cheng, Lin Zhang, Matthew Tak Vai Chan, Sunny Hei Wong, William Ka Kei Wu
Summary: Clostridioides difficile infection (CDI) is a common cause of nosocomial diarrhea. TcdB impairs lysosomal function in macrophages, leading to inflammation. Vitamin D-3 and carbamazepine protect against CDI by restoring lysosomal function and the expression of the transcription factor MITF in macrophages.
Article
Food Science & Technology
Masahiro Nagahama, Keiko Kobayashi, Sadayuki Ochi, Masaya Takehara
Summary: Clostridium botulinum C2 toxin requires lysosomal enzyme cathepsin B (CTSB) for effective cellular entry, with cysteine protease and aspartyl protease having different effects on cell rounding and extracellular ASMase activity induced by C2 toxin.
Article
Endocrinology & Metabolism
Kali M. Pruss, Fatima Enam, Eric Battaglioli, Mary DeFeo, Oscar R. Diaz, Steven K. Higginbottom, Curt R. Fischer, Andrew J. Hryckowian, William Van Treuren, Dylan Dodd, Purna Kashyap, Justin L. Sonnenburg
Summary: The pathogen Clostridioides difficile (Cd) can colonize the gut even without causing any symptoms of the disease. The prevalence of asymptomatic colonization by toxigenic Cd in healthy populations is high. In this study, researchers analyzed the gut microbiome of mice resistant to Cd infection and inflammation and found increased expression of arginine and ornithine metabolic pathways. They also identified a specific operon consistently upregulated in non-toxigenic Cd strains. Through metabolomics and genetic analysis, the researchers demonstrated that both diet- and host-derived sources of ornithine provide a competitive advantage to Cd, suggesting a mechanism for Cd persistence in a non-inflammatory, healthy gut.
Article
Chemistry, Analytical
Waleed A. Hassanain, Julia Spoors, Christopher L. Johnson, Karen Faulds, Neil Keegan, Duncan Graham
Summary: The study introduces a novel testing platform for the rapid and simultaneous detection of two specific biomarkers of Clostridium difficile infection, providing a rapid, selective, sensitive, and cost-effective clinical evaluation method for CDI.
Article
Pharmacology & Pharmacy
Kristin Werner, Detlef Neumann, Roland Seifert
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
(2016)
Article
Pharmacology & Pharmacy
Eva J. Wunschel, Bastian Schirmer, Roland Seifert, Detlef Neumann
FRONTIERS IN PHARMACOLOGY
(2017)
Article
Immunology
Bastian Schirmer, Luisa Bringmann, Roland Seifert, Detlef Neumann
FRONTIERS IN IMMUNOLOGY
(2018)
Article
Pharmacology & Pharmacy
Bastian Schirmer, Luisa Lindemann, Kaya Saskia Bittkau, Rukijat Isaev, Daniela Boesche, Malte Juchem, Roland Seifert, Detlef Neumann
JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
(2020)
Article
Geriatrics & Gerontology
Domiziana Costamagna, Robin Duelen, Fabio Penna, Detlef Neumann, Paola Costelli, Maurilio Sampaolesi
JOURNAL OF CACHEXIA SARCOPENIA AND MUSCLE
(2020)
Article
Oncology
Bastian Schirmer, Tamina Rother, Inga Bruesch, Andre Bleich, Christopher Werlein, Danny Jonigk, Roland Seifert, Detlef Neumann
Review
Biochemistry & Molecular Biology
Bastian Schirmer, Detlef Neumann
Summary: Histamine is a versatile mediator involved in various physiological processes, including the regulation of inflammation. Approved compounds targeting histamine receptors are used for allergic inflammation, gastric acid reduction, and narcolepsy treatment. H4R ligands are still under investigation for inflammatory diseases, showing moderate effects so far.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Editorial Material
Pharmacology & Pharmacy
Detlef Neumann, Roland Seifert
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
(2021)
Article
Pharmacology & Pharmacy
Jasper Carsten Schrammel, Martin Koenig, Miriam Frommer, Kaya Saskia Andersen, Marla Kirsten, Roland Seifert, Detlef Neumann, Bastian Schirmer
Summary: By studying cell lines, it was found that colon epithelial cells have functional expression of H4 receptors, which further confirmed the involvement of H4 receptors in inflammatory bowel disease and associated colon cancer. However, the transferability of these data to humans is uncertain. The expression of histamine receptor subtypes varied among different cell lines, and genetic modification is necessary for detailed functional analysis in human colon-derived cell lines.
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
(2023)
Meeting Abstract
Cell Biology
T. Rother, B. Schirmer, R. Seifert, D. Neumann
INFLAMMATION RESEARCH
(2019)
Meeting Abstract
Pharmacology & Pharmacy
T. Rother, B. Schirmer, R. Seifert, D. Neumann
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
(2019)
Meeting Abstract
Cell Biology
D. Neumann
INFLAMMATION RESEARCH
(2018)
Meeting Abstract
Pharmacology & Pharmacy
D. Neumann, E. Wunschel, B. Schirmer
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
(2017)
Meeting Abstract
Pharmacology & Pharmacy
B. Schirmer, T. Rezniczek, R. Seifert, D. Neumann
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
(2016)
Meeting Abstract
Pharmacology & Pharmacy
K. Bittkau, L. Bringmann, D. Boesche, R. Isaev, B. Schirmer, R. Seifert, D. Neumann
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
(2018)