Review
Chemistry, Multidisciplinary
Faranak Mankavi, Rana Ibrahim, Hongjun Wang
Summary: This article summarizes the recent advances in nerve guidance conduits (NGCs) for peripheral nerve regeneration and repair, including biomaterial innovations, structural design, advanced fabrication technologies, and cellular responses to biomimetic cues.
Article
Engineering, Biomedical
Takuya Yokoi, Takuya Uemura, Kiyohito Takamatsu, Kosuke Shintani, Ema Onode, Shunpei Hama, Yusuke Miyashima, Mitsuhiro Okada, Hiroaki Nakamura
Summary: The study showed that mouse iPSC-derived neurospheres transplanted with nerve conduits promoted peripheral nerve regeneration in mice by migrating into regenerating axons, surviving as Schwann-like cells, and upregulating expression levels of nerve regeneration-associated trophic factors such as ATF3, BDNF, and GDNF.
BIO-MEDICAL MATERIALS AND ENGINEERING
(2021)
Article
Multidisciplinary Sciences
Ema Onode, Takuya Uemura, Kiyohito Takamatsu, Takuya Yokoi, Kosuke Shintani, Shunpei Hama, Yusuke Miyashima, Mitsuhiro Okada, Hiroaki Nakamura
Summary: This study found that nerve conduits coated with human induced pluripotent stem cell (hiPSC)-derived neurospheres can promote nerve regeneration and functional recovery in repairing rat sciatic nerve defects. This nerve regeneration approach shows promise as a new clinical therapy for treating peripheral nerve defects.
SCIENTIFIC REPORTS
(2021)
Review
Biochemistry & Molecular Biology
Andrea Lavorato, Stefania Raimondo, Marina Boido, Luisa Muratori, Giorgia Durante, Fabio Cofano, Francesca Vincitorio, Salvatore Petrone, Paolo Titolo, Fulvio Tartara, Alessandro Vercelli, Diego Garbossa
Summary: The study discusses the features and applications of different types of mesenchymal stem cells in peripheral nerve regeneration after nerve injury. ADSCs are considered the most suitable MSCs for recovery from peripheral nerve lesions due to their excellent support and stimulation of axonal growth, remarkable paracrine activity, differentiation potential, low immunogenicity, and high survival rate.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell & Tissue Engineering
Toru Kondo
Summary: The study demonstrates that the DHODH inhibitor BRQ induces differentiation in mouse PSCs, preventing teratoma formation, while showing low toxicity to normal cells. This suggests that DHODH inhibitors like BRQ could be crucial for successful PSC-based therapy.
Review
Biochemistry & Molecular Biology
Matin Chehelgerdi, Fereshteh Behdarvand Dehkordi, Mohammad Chehelgerdi, Hamidreza Kabiri, Hosein Salehian-Dehkordi, Mohammad Abdolvand, Sharareh Salmanizadeh, Mohsen Rashidi, Anoosha Niazmand, Saba Ahmadi, Sara Feizbakhshan, Saber Kabiri, Nasimeh Vatandoost, Tayebeh Ranjbarnejad
Summary: The emergence of iPSCs has revolutionized stem cell research and opened up new avenues for cancer treatment. iPSCs provide a unique platform for investigating the origin of cancer and can be used for personalized treatment and drug testing. This review article discusses the science behind iPSCs, the limitations and challenges in iPSC-based cancer therapy, ethical and social implications, and a comparative analysis with other stem cell types for cancer treatment. It also explores the applications of iPSCs in tumorigenesis and the future of iPSC research in this field.
Article
Biochemistry & Molecular Biology
Takuya Yokoi, Takuya Uemura, Kiyohito Takamatsu, Ema Onode, Kosuke Shintani, Shunpei Hama, Yusuke Miyashima, Mitsuhiro Okada, Hiroaki Nakamura
Summary: This study evaluated the long-term survival of transplanted mouse iPSC-derived neurospheres with nerve conduits using in vivo imaging. Results showed that the transplanted iPSC-derived neurospheres survived for 365 days in immunosuppressed allograft models, but only for at least 14 days in non-immunosuppressed allograft models. This study highlights the potential for iPSC-based cell therapy in promoting nerve regeneration in peripheral nerve injury.
BIOCHEMISTRY AND BIOPHYSICS REPORTS
(2021)
Review
Biochemistry & Molecular Biology
Aline Yen Ling Wang
Summary: Induced pluripotent stem cells (iPSCs) and iPSC-derived exosomes demonstrate therapeutic effects through paracrine mechanisms, with potential applications in various disease models.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Glyn Nigel Stacey, Jie Hao
Summary: In recent years, significant progress has been made in China in establishing hPSCs for the manufacture of cell therapies. The National Stem Cell Resource Center and its partner organizations have over 600 hPSC lines formally recorded, with 47 of them also registered for European Commission funded research projects. This national and international coordination aims to promote the translation of Chinese hPSC-based products into clinical use according to established standards.
CELL PROLIFERATION
(2022)
Review
Biochemistry & Molecular Biology
Agnieszka Fus-Kujawa, Barbara Mendrek, Anna Trybus, Karolina Bajdak-Rusinek, Karolina L. Stepien, Aleksander L. Sieron
Summary: Induced pluripotent stem cells (iPSCs) show potential in gene therapy and disease modeling. To safely apply this technology in clinical settings, optimal reprogramming factors and vectors must be considered to reduce the risk of insertional tumorigenesis.
Review
Pharmacology & Pharmacy
Lu Zhang, Xiao-Jing-Nan Ma, Yuan-Yuan Fei, Heng-Tong Han, Jun Xu, Lu Cheng, Xun Li
Summary: The liver has the ability to repair itself, but pathological changes can impair its regenerative capacity and lead to liver failure. Mesenchymal stem cells (MSCs) have shown promise in liver regeneration, and induced pluripotent stem cells have made progress in organoid establishment and drug screening.
PHARMACOLOGY & THERAPEUTICS
(2022)
Article
Cell Biology
Julius M. Mayer, Christian Krug, Maximilian M. Saller, Annette Feuchtinger, Riccardo E. Giunta, Elias Volkmer, Thomas Holzbach
Summary: Recent research highlights the beneficial effects of hypoxia-preconditioned adipose-derived multipotent stem/progenitor cells (ADSPCs) in peripheral nerve regeneration. The study investigated the use of a custom-made fibrin conduit seeded with hypoxic pre-conditioned ADSPCs for autologous sciatic nerve graft in rats. Compared to other treatment modes, the hypoxic pre-conditioned group showed faster functional regeneration, increased axon outgrowth/branching and density, remyelination, and reduced connective tissue area. Further studies are needed to explore the cellular mechanism and potential clinical applications.
NEURAL REGENERATION RESEARCH
(2023)
Article
Cell Biology
Tzu-Cheng Sung, Yi-Peng Jiang, Jhe-Yu Hsu, Qing-Dong Ling, Hao Chen, Suresh S. Kumar, Yung Chang, Shih-Tien Hsu, Qingsong Ye, Akon Higuchi
Summary: This study successfully generated universal hiPSCs without gene editing and evaluated their resistance to immune reactions, demonstrating that the cells maintained stable characteristics even after continuous passages.
CELL PROLIFERATION
(2021)
Article
Computer Science, Interdisciplinary Applications
Yusuke Hayashi, Ikki Horiguchi, Masahiro Kino-oka, Hirokazu Sugiyama
Summary: This study presents a model-based assessment of temperature profiles in slow freezing for human induced pluripotent stem cells, which can estimate cell survival rate and required freezing time based on given freezing temperature profiles to achieve quality and productivity objectives. Simulation results indicate different cooling rates in dehydration, nucleation-promoting, and further cooling zones.
COMPUTERS & CHEMICAL ENGINEERING
(2021)
Review
Cell Biology
Michal S. Lach, Monika A. Rosochowicz, Magdalena Richter, Inga Jagiello, Wiktoria M. Suchorska, Tomasz Trzeciak
Summary: The development of induced pluripotent stem cells has opened up unlimited possibilities for regenerative medicine. This technology can be used to treat skeletal diseases such as osteoarthritis and has the potential to replace current short-term solutions. Additionally, using patient-specific tissue for reprogramming purposes can provide a platform for drug screening and modeling skeletal abnormalities.