期刊
JOURNAL OF SOLID STATE ELECTROCHEMISTRY
卷 20, 期 5, 页码 1263-1270出版社
SPRINGER
DOI: 10.1007/s10008-016-3122-x
关键词
DNA methylation; Multiwalled carbon nanotubes; beta-cyclodextrin; 5-methylcytosine; Electrochemical assay
资金
- National Scientific Foundation of China (NSFC) [91417301, 21375101, 60801020]
- Fundamental Research Funds for the Central Universities [2042014kf0295]
- Natural Science Foundation of Hubei Province [ZRY2014000492]
- Wuhan Science and Technology Bureau [20140601010057]
We describe a method for detecting DNA methylation. It is based on direct oxidation of DNA bases at a glassy carbon electrode (GCE) modified with film of a multiwalled carbon nanotube-beta-cyclodextrin composite. This nano-structured film causes a strong enhancement on the oxidation current of DNA bases due to its large effective surface area and extraordinary electronic properties. Well-defined peaks were obtained as a result of electro-oxidation of guanine (at 0.67 V), adenine (at 0.92 V), thymine (at 1.11 V), cytosine (at 1.26 V), and 5-methylcytosine (at 1.13 V; all data vs. saturated calomel electrode (SCE)). The potential difference between 5-methylcytosine and cytosine (130 mV) is large enough to enable reliable simultaneous determination and analysis. The interference by thymine can be eliminated by following the principle of complementary pairing between purine and pyrimidine bases in DNA. The modified electrode was successfully applied to the evaluation of 5-methylcytosine in a fish sperm DNA, the methylation level of cytosine was found to be 7.47 %, and the analysis process took less than 1 h.
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