期刊
CELL STRESS & CHAPERONES
卷 20, 期 2, 页码 309-319出版社
SPRINGER
DOI: 10.1007/s12192-014-0550-3
关键词
Alzheimer's disease; Hsp90; Geldanamycin; p70S6K; Learning; Memory
类别
One of the neuropathological hallmarks of Alzheimer's disease (AD) is the accumulation of beta-amyloid peptides (A beta) in senile plaques. A beta-induced oxidative stress is believed to be responsible for degeneration and apoptosis of neurons and consequent cognitive and memory deficits. Here, we investigated the possible neuroprotective effect of the heat shock protein 90 (Hsp90) inhibitor geldanamycin (GA) against amyloid pathogenesis in adult male Wistar rats. GA or vehicle was injected into the lateral cerebral ventricles of rats 24 h before injection of A beta (1-42) in CA1 area of hippocampus. The learning and memory of the rats were assessed 7 days after injection of A beta using passive avoidance (PA) task. As potential contributing factors in A beta-induced memory decline, we evaluated apoptotic markers and also used terminal-transferase UTP nick end labeling (TUNEL) technique to detect apoptosis in the hippocampus of A beta-injected rats. Our behavioral data suggest that GA pretreatment can significantly suppress memory deficits in A beta-injected rats. There was also not only a marked increase in Hsp70 level but also upregulated 70 kDa ribosomal protein S6 kinase (p70S6K) in the hippocampus of GA-treated groups with a reduction in apoptotic factors including caspase-3, poly (ADP-ribose) polymerase, Bax/Bcl-2 ratio, and TUNEL-positive cells as well. Thus, we conclude that GA exerts its protective effects against A beta (1-42) toxicity and memory deficits, at least in part, by upregulating of Hsp70 and P70S6K.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据