Article
Biochemistry & Molecular Biology
Anastasiya Matveeva, Alexander Ryabchenko, Viktoria Petrova, Daria Prokhorova, Evgenii Zhuravlev, Alexander Zakabunin, Artem Tikunov, Grigory Stepanov
Summary: Research on Cas9 nucleases from different organisms has the potential to advance genome engineering and gene therapy tools. Thermophilic Cas9 nucleases, which have varying activity temperature ranges and PAM preferences, are expanding. This study focuses on constructing a compact Cas9 nuclease that displays nuclease activity in the 37-60 degrees C range.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Xueli Zhang, Chaohui Zhang, Caijiao Liang, Bizhou Li, Fanmei Meng, Yuncan Ai
Summary: Bacteriophages, the most abundant organisms in the biosphere, have been sequenced extensively. However, the study of bacteriophage functional genomics has been hindered by a lack of effective research methods. This study designed a phage genome editing platform based on the CRISPR-Cas9 system, and successfully achieved gene editing in Vibrio natriegens phage TT4P2. This platform has the potential to advance research on phage gene diversity and accelerate the development of phage synthetic biology and nanotechnology.
MICROBIOLOGY SPECTRUM
(2022)
Article
Multidisciplinary Sciences
Joonbum Lee, Dong-Hwan Kim, Madeline C. Karolak, Sangsu Shin, Kichoon Lee
Summary: In this study, we successfully generated genome-edited chicken and duck lines without the conventional PGC-mediated procedures by injecting an adenovirus containing the CRISPR-Cas9 system into avian blastoderms.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biotechnology & Applied Microbiology
Xiaoyu Zhang, Gencheng Xu, Chaohua Cheng, Lei Lei, Jian Sun, Ying Xu, Canhui Deng, Zhigang Dai, Zemao Yang, Xiaojun Chen, Chan Liu, Qing Tang, Jianguang Su
Summary: This study successfully generated gene-edited plants in Cannabis sativa using an Agrobacterium-mediated transformation method, improving shoot regeneration efficiency and editing the phytoene desaturase gene with CRISPR/Cas9 technology.
PLANT BIOTECHNOLOGY JOURNAL
(2021)
Article
Biochemical Research Methods
Hyun-Woo Je, Chang-Hun Ji, Jun-Yong Kim, Hahk-Soo Kang
Summary: The CRISPR/Cas9 system faces toxicity issues in Streptomyces genome engineering due to overexpression of the Cas9 protein. The CaExTun platform has been developed to optimize Cas9 expression for reduced toxicity while maintaining sufficient DSB activity. This tool enables rapid optimization of the CRISPR/Cas9 system in a species-specific manner, facilitating genome engineering efforts in Streptomyces.
ACS SYNTHETIC BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Hong You, Johannes U. Mayer, Rebecca L. Johnston, Haran Sivakumaran, Shiwanthi Ranasinghe, Vanessa Rivera, Olga Kondrashova, Lambros T. Koufariotis, Xiaofeng Du, Patrick Driguez, Juliet D. French, Nicola Waddell, Mary G. Duke, Wannaporn Ittiprasert, Victoria H. Mann, Paul J. Brindley, Malcolm K. Jones, Donald P. McManus
Summary: CRISPR/Cas9-mediated genome editing has shown great potential for genetic modification of helminth parasites, as demonstrated by successful gene knock-in in Schistosoma mansoni eggs. This study confirmed the utility of CRISPR/Cas9 for functional genomics in schistosomes through combining CRISPR/Cas9 with single-stranded oligodeoxynucleotides (ssODNs).
Review
Chemistry, Multidisciplinary
Peng Yang, Athena Yue-Tung Lee, Jingjing Xue, Shih-Jie Chou, Calvin Lee, Patrick Tseng, Tiffany X. Zhang, Yazhen Zhu, Junseok Lee, Shih-Hwa Chiou, Hsian-Rong Tseng
Summary: The CRISPR/Cas9 genome editing system has revolutionized the field of therapeutic applications for genetic diseases and cancers. Efficient delivery of the system in vivo remains a significant challenge. Non-viral nano-vectors offer advantages over viral vectors and can be used to deliver CRISPR/Cas9 cargoes for targeted gene editing. The article highlights recent advances in nano-vector delivery for treating cancers and genetic diseases and proposes strategies for future development.
Review
Chemistry, Multidisciplinary
Li Duan, Limei Xu, Xiao Xu, Zhuan Qin, Xiaoying Zhou, Yin Xiao, Yujie Liang, Jiang Xia
Summary: Gene vectors deliver genetic materials or gene editing devices into cells for therapeutic protein production or gene correction, but barriers like cell membrane and degradation necessitate carriers for clinical gene therapy. Exosomes, engineered to encapsulate and deliver nucleic acids, and their fusion with liposomes increase loading capacity and maintain targeting capability, showing promise for future gene delivery applications in therapy.
Review
Biochemistry & Molecular Biology
Shuai Ding, Jinfeng Liu, Xin Han, Mengfan Tang
Summary: The CRISPR/Cas9 system, a highly efficient and accurate gene editing tool, has been widely applied in cancer research. Its utilization in CAR-T-cell therapy, tumor model establishment, and gene and drug target screening has propelled the investigation of cancer molecular mechanisms and the advancement of precision medicine. However, the therapeutic potential of genome editing remains underexplored and may pose risks of additional genetic mutations.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Christopher E. Denes, Alexander J. Cole, Yagiz Alp Aksoy, Geng Li, Graham Gregory Neely, Daniel Hesselson
Summary: Genome modification holds great potential for disease prevention or treatment, with CRISPR/Cas9 techniques showing promise in altering disease-relevant genes. Competition among DNA repair pathways can lead to undesirable editing outcomes, but small molecule modulators and engineered CRISPR/Cas proteins have been shown to enhance precision editing efficacy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cell Biology
Gemma Llargues-Sistac, Laia Bonjoch, Sergi Castellvi-Bel
Summary: The use of next-generation sequencing (NGS) technologies has greatly advanced our understanding of the mutational landscape of complex human diseases such as cancer. Haploid human cell models, such as the HAP1 cell line, have emerged as valuable tools for functional gene studies, especially in combination with CRISPR-Cas9 gene editing technology. This review explores the recent applications of the HAP1 cell line in functional genetic studies and high-throughput genetic screens, highlighting its potential to enhance our understanding of gene function and the genetic basis of human diseases identified through NGS technologies, and its implications for clinical practice and patient care.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Review
Plant Sciences
Michelle Roenspies, Patrick Schindele, Holger Puchta
Summary: The powerful CRISPR/Cas system has revolutionized plant breeding by enabling precise genome manipulation, moving beyond simply knocking in or out single genes to inducing targeted chromosomal rearrangements. This technique has the potential to transform plant breeding by altering genetic linkages between traits and overcoming natural obstacles to breeding processes like inversions. Recent breakthroughs in chromosome engineering in plants suggest potential applications in shaping plant chromosomes based on breeding needs.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Biology
Jian-Ping Zhang, Zhi-Xue Yang, Feng Zhang, Ya-Wen Fu, Xin-Yue Dai, Wei Wen, Beldon Zhang, Hannah Choi, Wanqiu Chen, Meredith Brown, David Baylink, Lei Zhang, Hongyu Qiu, Charles Wang, Tao Cheng, Xiao-Bing Zhang
Summary: Research shows that HDAC inhibitors can increase the efficiency of genome editing, especially when dealing with silent genes. HDR efficiency improvement is more significant at closed loci.
SCIENCE CHINA-LIFE SCIENCES
(2021)
Article
Plant Sciences
Pawan Mainkar, Tushar Kashinath Manape, Viswanathan Satheesh, Sivalingam Anandhan
Summary: This study reports the successful establishment of a CRISPR/Cas9-mediated genome editing protocol in onions, using the AcPDS gene as an example. Through transformation and cultivation of calli, different phenotypes were observed in regenerated shoots, with some exhibiting albino or chimeric characteristics. PCR and deep amplicon sequencing confirmed the successful editing of the AcPDS gene.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Hongmei Nie, Yu Shi, Xueqing Geng, Guoming Xing
Summary: Polygalacturonase (PG) gene is a key candidate for regulating fruit firmness in tomatoes, and using CRISPR/Cas9 technology to induce mutations in the SlPG gene can delay fruit softening. Additionally, transgene-free tomato plants with mutated SlPG alleles were identified, providing materials for further research on SlPG functions and the molecular mechanism of fruit softening in tomatoes.
FRONTIERS IN PLANT SCIENCE
(2022)