期刊
JOURNAL OF SEPARATION SCIENCE
卷 40, 期 1, 页码 228-250出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201600962
关键词
Microchip electrophoresis; Microfluidics; Peptides; Proteins
资金
- Czech Science Foundation [15 01948S]
- Czech Academy of Sciences [RVO 61388963]
This review presents the developments and applications of microchip electromigration methods in the separation and analysis of peptides and proteins in the period 2011-mid-2016. The developments in sample preparation and preconcentration, microchannel material, and surface treatment are described. Separations by various microchip electromigration methods (zone electrophoresis in free and sieving media, affinity electrophoresis, isotachophoresis, isoelectric focusing, electrokinetic chromatography, and electrochromatography) are demonstrated. Advances in detection methods are reported and novel applications in the areas of proteomics and peptidomics, quality control of peptide and protein pharmaceuticals, analysis of proteins and peptides in biomatrices, and determination of physicochemical parameters are shown.
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