Article
Chemistry, Analytical
Firouzeh Hassani Moghadam, Mohammad A. Taher, Hassan Karimi-Maleh
Summary: The glassy carbon electrode (GCE) amplified with single-wall carbon nanotubes (SWCNTs) and ds-DNA was successfully utilized for voltammetric sensing of doxorubicin with a low detection limit. Molecular docking study confirmed the interaction of doxorubicin with DNA through intercalation mode, consistent with experimental results. The ds-DNA/SWCNTs/GCE showed excellent detection performance for doxorubicin and powerful ability for determination of the drug in injection samples with acceptable recovery data.
Review
Chemistry, Analytical
Jiancheng Zhang, Lixia Lu, Zhenguo Zhang, Liguo Zang
Summary: Cell-based electrochemical biosensors play a crucial role in detecting food hazards, offering high precision and non-destructive characteristics. Strategies such as cell immobilization and 3D culture on electrodes are effective in enhancing efficiency.
Article
Biophysics
Jaehan Ko, Jeongwoo Ham, Hwajung Lee, Kangwon Lee, Won-Gun Koh
Summary: The study introduces a new platform that integrates a 3D cell culture scaffold with a SERS-based biosensor for monitoring protein markers secreted by stem cells without the need for periodic cell collection. The system can sensitively monitor osteogenic differentiation of stem cells and detect multiple protein markers, providing fast and accurate analysis capabilities.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Jing-Xian Wang, Xue-Jin Yang, Yan-Zhai Wang, Kai Yang, Huayou Chen, Yang-Chun Yong
Summary: A signal amplification system for electrochemical sensing was established using bio-nanohybrid cells based on bacterial self-assembly and biomineralization. This system showed high transmembrane electron transfer efficiency and achieved ultrasensitive detection of riboflavin.
ANALYTICAL CHEMISTRY
(2022)
Article
Environmental Sciences
Ademola Adekunle, Abraham Gomez Vidales, Lyne Woodward, Boris Tartakovsky
Summary: The study introduces a soft sensor approach for real-time evaluation of the internal state of an MFC biosensor, based on estimating the equivalent circuit model parameters in real time. Experimental results demonstrate that this soft sensor approach can serve as a replacement for invasive EIS measurements and provides additional information for earlier detection of toxicity.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Multidisciplinary Sciences
T. Deckers, J-M Aerts, V Bloemen
Summary: In this study, a novel cell detachment feature was developed using lens-free imaging technology, allowing quantitative monitoring of the detachment response and determination of the optimal time for enzyme inhibition.
SCIENTIFIC REPORTS
(2022)
Article
Biology
Dennis May, Sangwon Yun, David G. Gonzalez, Sangbum Park, Yanbo Chen, Elizabeth Lathrop, Biao Cai, Tianchi Xin, Hongyu Zhao, Siyuan Wang, Lauren E. Gonzalez, Katie Cockburn, Valentina Greco
Summary: Stem cell differentiation involves dramatic changes in gene expression and global remodeling of chromatin architecture. In this study, a quantitative pipeline using fluorescently-tagged histones and longitudinal imaging was developed to track chromatin compaction changes in live mice. Analysis of epidermal stem cells reveals that cell-to-cell chromatin compaction heterogeneity is independent of cell cycle status and reflective of differentiation status. Furthermore, live imaging of Keratin-10 nascent RNA shows that transcription precedes global chromatin compaction changes during differentiation. These findings highlight the dynamic transcriptional states and gradual chromatin rearrangement involved in stem cell differentiation.
Article
Chemistry, Analytical
Fangming Chen, Qian Guo, Qiaoyu Yang, Yao Meng, Mingrui Jiang, Jian Wang, De-Wen Zhang
Summary: Label-free electrochemical visualization of cancer cell apoptosis is achieved using a light-addressable electrochemical sensor. The study demonstrates that the AC photocurrent on apoptotic MCF-7 cells increases, indicating heterogeneity in apoptosis degrees. This new method provides potential applications in apoptosis-associated drug screening and efficacy evaluation.
ANALYTICAL CHEMISTRY
(2023)
Article
Environmental Sciences
Xiaoou Wei, Chao Liu, Zhihua Li, Quancai Sun, Xinai Zhang, Yanxiao Li, Wen Zhang, Jiyong Shi, Xiaodong Zhai, Di Zhang, Xiaobo Zou
Summary: In this research, a label-free electrochemical cell-based biosensor was developed for the in vitro toxicity assessment of thiram. The biosensor exhibited excellent sensitivity, stability, and reliability in measuring the cytotoxicity of thiram, and the toxicity of thiram residue in food, even at low residual concentrations, cannot be ignored.
Article
Biophysics
Hongwei Chu, Xiaokang Hu, Cheng-Yu Lee, Anning Zhang, Yang Ye, Yuxin Wang, Yangyang Chen, Xiao Yan, Xinzhong Wang, Jun Wei, Sisi He, Yingchun Li
Summary: This article introduces a novel electrochemical fabric for real-time monitoring of cytokines. The fabric is flexible, breathable, and can be applied to different body parts. It can also track other biomarkers by replacing the coupling aptamer, serving as a universal platform for sweat analysis. Rating: 9/10.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Biophysics
Yonghwan Kim, Dongtak Lee, Youngjun Seo, Hyo Gi Jung, Jae Won Jang, Dongsung Park, Insu Kim, Jaeheung Kim, Gyudo Lee, Kyo Seon Hwang, Seung-Hyun Kim, Sang Won Lee, Jeong Hoon, Dae Sung Yoon
Summary: In this study, an electrochemical biosensor based on cell membrane was developed, inspired by the pathological process of cholera toxin. The biosensor exhibited excellent detection performance, selectivity, and long-term stability. Overall, it has great potential for clinical application and cost-effective detection of cholera toxin in developing countries.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Multidisciplinary
Sungkyu Lee, Myeong Soo Kim, Kapil D. Patel, Hyojun Choi, Ramar Thangam, Jinho Yoon, Thomas Myeongseok Koo, Hee Joon Jung, Sunhong Min, Gunhyu Bae, Yuri Kim, Seong-Beom Han, Nayeon Kang, Minjin Kim, Na Li, Hong En Fu, Yoo Sang Jeon, Jae-Jun Song, Dong-Hwee Kim, Steve Park, Jeong-Woo Choi, Ramasamy Paulmurugan, Yun Chan Kang, Heon Lee, Qiang Wei, Vinayak P. Dravid, Ki-Bum Lee, Young Keun Kim, Heemin Kang
Summary: The density of ligand nanoassembly on Fe3O4 nanoparticle can be dynamically changed to control stem cell differentiation. Magnetic control of rising and falling ligand movements influences adhesion and differentiation processes through intermolecular interactions.
Article
Chemistry, Analytical
Yanran Liu, Yajie Yang, Yining Fan, Qi Zhao, Guanyue Gao, Jinfang Zhi
Summary: The feasibility of microbial electrochemical biosensors in marine bio-toxicity assessment was investigated. Two design strategies, disperse system and integrated microbial electrode, were discussed. The sensitivity of the biosensors to heavy metal ions and pesticides was determined.
Article
Chemistry, Applied
Muhammad Ihda H. L. Zein, Ari Hardianto, Irkham Irkham, Salma Nur Zakiyyah, Melania Janisha Devi, Ninie Suhana Abdul Manan, Abdullahi Umar Ibrahim, Yeni Wahyuni Hartati
Summary: The technological revolution in agriculture and food has led to the production of genetically modified crops that are high yield and less susceptible to pests and weather conditions. However, safety issues, including antibiotic residues, still persist in the sector. Aptasensors, which are biorecognition molecules, have been developed to detect antibiotics in food with high affinity and specificity.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2023)
Review
Biochemistry & Molecular Biology
Christophe Furger
Summary: This review presents four main cell-based approaches for quantitatively measuring the antioxidant effects of plant extracts, with the Nrf2/ARE gene reporter system currently standing out as the most standardized and high-throughput method, while AOP1 appears as the most promising tool for evaluating direct cellular ROS scavenging effects at an industrial scale.
Review
Chemistry, Physical
Cheol-Hwi Kim, Tae-Hyung Kim
Article
Chemistry, Physical
Jeong Ah Park, Jinmyeong Kim, Soo Min Kim, Hiesang Sohn, Chulhwan Park, Tae-Hyung Kim, Jin-Ho Lee, Min-Ho Lee, Taek Lee
Summary: This study proposed an electrochemical biosensor using a multifunctional DNA four-way junction (4WJ) and carboxyl molybdenum disulfide (carboxyl-MoS2) hybrid material for precise detection of H1N1, showing excellent specificity and selectivity.
Review
Biochemistry & Molecular Biology
Intan Rosalina Suhito, Kyeong-Mo Koo, Tae-Hyung Kim
Summary: Electrochemical sensors are widely used for detecting biomolecules and cells with advantages such as label-free and non-destructive detection, high sensitivity, and selectivity. Recent studies focus on improving sensor performance and expanding applications through various nanomaterials and biomaterials.
Article
Chemistry, Physical
Da-Seul Kim, Jun-Kyu Lee, Ji-Won Jung, Seung-Woon Baek, Jun Hyuk Kim, Yun Heo, Tae-Hyung Kim, Dong Keun Han
Summary: The study combined porous PLGA scaffold with magnesium hydroxide and bone-extracellular matrix, as well as incorporated bioactive polydeoxyribonucleotide to create a PMEP scaffold with pro-osteogenic and pro-angiogenic effects. The results showed that the PMEP scaffold had superior biological properties for human bone-marrow mesenchymal stem cells adhesion, proliferation, and osteogenic differentiation.
Review
Chemistry, Analytical
Kwang-Ho Lee, Tae-Hyung Kim
Summary: The article discusses the widespread use of multicellular tumor spheroids (MCTs) in the biomedical field and their connection to drug screening, emphasizing the importance of mimicking the tumor microenvironment for understanding cell-cell and cell-matrix interactions.
Article
Chemistry, Analytical
Joon-Ha Park, Yeon-Woo Cho, Tae-Hyung Kim
Summary: This article reviews the recent advances in optical surface plasmon resonance (SPR) sensor technology for pathogen biosensing. This technology utilizes nano-sized materials for specific pathogen detection, ensuring high selectivity and real-time results, contributing to instantaneous diagnosis and timely treatment.
Article
Biochemistry & Molecular Biology
So-Yeon Park, Da-Seul Kim, Hyun-Mun Kim, Jun-Kyu Lee, Dong-Youn Hwang, Tae-Hyung Kim, Seungkwon You, Dong Keun Han
Summary: Mesenchymal stem cells (MSCs) have been used in clinical studies due to their versatility and low immunogenicity. However, safety issues remain a concern. Recently, extracellular vesicles (EVs) derived from MSCs have been explored as an alternative. EVs are nano-sized particles released by cells and have similar biological functions as their origin cells. They carry bioactive molecules and facilitate tissue regeneration. EVs derived from adipose-derived MSCs (ADMSC) also have neuroprotective and neurogenesis effects. This study aimed to enhance the neural differentiation ability of ADMSC-derived EVs by investigating the neurogenic-differentiation process. The results showed that differentiated EVs (dEVs) obtained from neurogenesis conditioned media increased neurogenic ability by altering microRNA expression and cytokine composition. dEVs promoted neuronal differentiation of neural progenitor cells in vitro, indicating their potential in EV-based therapy for neurological disorders.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Polymer Science
Sang Ik Lee, Yoon Young Choi, Seong Goo Kang, Tae Hyeon Kim, Ji Wook Choi, Young Jae Kim, Tae-Hyung Kim, Taewook Kang, Bong Geun Chung
Summary: A microfluidic-based droplet system was developed to generate uniform 3D tumor spheroids for drug screening applications. The system allowed high-throughput generation of spheroids with various cellular ratios, providing a potentially powerful tool for anti-cancer drug analysis.
Article
Pharmacology & Pharmacy
Seung-Woon Baek, Da-Seul Kim, Duck Hyun Song, Semi Lee, Jun-Kyu Lee, So-Yeon Park, Jun Hyuk Kim, Tae-Hyung Kim, Chun Gwon Park, Dong Keun Han
Summary: This study proposes a coating system to deliver hydrophilic bioactive agents to biodegradable vascular scaffolds (BVS). The coating system enhances re-endothelialization and anti-inflammation, providing a new platform for the treatment of cardiovascular diseases (CVDs).
Review
Chemistry, Analytical
Min-Ji Kang, Yeon-Woo Cho, Tae-Hyung Kim
Summary: Non-invasive, non-destructive, and label-free sensing techniques are required for real-time monitoring of stem cell differentiation. Conventional methods are invasive and time-consuming, while electrochemical and optical techniques allow non-invasive qualitative identification and quantitative analysis of stem cell differentiation. Nanomaterials and micromaterials with cell-friendly properties can greatly improve the performance of biosensors for specific stem cell differentiation. This review focuses on such materials and aims to motivate further research for practical evaluation and efficient therapy of stem cell differentiation.
Review
Chemistry, Analytical
Joon-Ha Park, Yun-Sik Eom, Tae-Hyung Kim
Summary: In recent years, there has been an increasing demand for highly sensitive and selective biosensors for neurotransmitters due to advancements in science and technology. Real-time sensing plays a crucial role in preventing neurological and cardiovascular diseases effectively. This review summarizes the latest progress in aptamer-based biosensor technology, focusing on various biomaterials used to ensure optimal performance and high selectivity. Its aim is to further advance aptamer-based biosensor technology.
Review
Engineering, Biomedical
Yun-Sik Eom, Joon-Ha Park, Tae-Hyung Kim
Summary: Stem cells' unique characteristics of self-renewal and differentiation have opened up various biomedical applications. Effective methods to control stem cell differentiation are crucial due to potential hazards of uncontrolled differentiation. Recent research has successfully used porous functional material-based drug delivery systems to regulate stem cell differentiation, showing promise for biomedical applications.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2023)
