期刊
CELL PROLIFERATION
卷 48, 期 5, 页码 593-599出版社
WILEY
DOI: 10.1111/cpr.12207
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资金
- National Nature Science Foundation of China [81301964]
- Postgraduate Student Foundation for New Teacher from the Ministry of Education of China [20133601120013]
- Foundation from Department of Education of Jiangxi Province [GJJ14215, GJJ14194]
- Province National Natural Science Foundation of Jiangxi [20151BAB215023]
- Foundation for Post-Doctor Research Project of Jiangxi Province, China
ObjectivesMicroRNAs (miRNAs) are endogenous small non-coding RNAs that regulate proteins and mRNAs for degradation or translational suppression. Up to now, the role of miR-372 in renal cell carcinoma has remained unknown; in this study, we have aimed to reveal its functional importance in this tumour. Materials and methodsqRT-PCR was performed to measure expression levels of miR-372 in renal cell carcinoma cell lines and tissues. CCK-8 and an invasion assay were performed to measure its functional role. Luciferase assays, qRT-PCR and western blotting were performed to discover miR-372s target gene. ResultsWe demonstrated that miRNA-372 was down-regulated in renal cell carcinoma cell lines and tissue specimens; its over-expression inhibited cell proliferation and invasion. Moreover, we showed that miRNA-372 repressed insulin-like growth factor 2 mRNA-binding protein 1 (IGF2BP1) expression by directly interacting with its putative binding site at the 3-UTR. Furthermore, ectopic expression of IGF2BP1 significantly reversed suppression of cell proliferation and invasion caused by miR-372 over-expression. ConclusionsOur data indicated that miR-372 seemed to function as a tumour suppressor in renal cell carcinoma progression by inhibiting the IGF2BP1 expression.
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