Pyrrolizidine Alkaloid Extraction and Analysis: Recent Updates

Pyrrolizidine alkaloids are natural secondary metabolites that are mainly produced in plants, bacteria, and fungi as a part of an organism's defense machinery. These compounds constitute the largest class of alkaloids and are produced in nearly 3% of flowering plants, most of which belong to the Asteraceae and Boraginaceae families. Chemically, pyrrolizidine alkaloids are esters of the amino alcohol necine (which consists of two fused five-membered rings including a nitrogen atom) and one or more units of necic acids. Pyrrolizidine alkaloids are toxic to humans and mammals; thus, the ability to detect these alkaloids in food and nutrients is a matter of food security. The latest advances in the extraction and analysis of this class of alkaloids are summarized in this review, with special emphasis on chromatographic-based analysis and determinations in food.

Automated summary

Pyrrolizidine alkaloids are natural secondary metabolites found in many plants, which can be toxic to humans and mammals. Detecting these alkaloids in food is crucial for food security. This review summarizes the latest advances in extraction and analysis methods, with a focus on chromatographic-based analysis and determinations in food.